Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 117858
• Name: oriT_pWP7-S18-CRE-03_4
• Organism: Klebsiella sp. WP7-S18-CRE-03
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP022199 (12347..12483 [-], 137 nt)
• oriT length: 137 nt
• IRs (inverted repeats): 28..33, 37..42 (ACATGC..GCATGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 137 nt

>oriT_pWP7-S18-CRE-03_4
CGAATGGGCAGGCGGGGATTGGCAGGAACATGCGTAGCATGTGTATGACAATCCGCATCCTGTATTGCTCAACCACTCTACTATCATATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCATAAAAGGCG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_H7R55_RS25575_pWP7-S18-CRE-03_4

• ID: 11347
• GenBank: WP_182930712
• Name: traI_H7R55_RS25575_pWP7-S18-CRE-03_4
• UniProt ID: _
• Length: 890 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 890 a.a.
• Molecular weight: 101900.39 Da
• Isoelectric Point: 9.7193

>WP_182930712.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [unclassified Klebsiella]
MIVKKVPNPKKSASKAQRIGQLTSYVRSPESESPQEKCLYAGARGFMMDDPKSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGWKDHQAIYGLHSDTDNIHLHIVINRVHPETLKIVEKNR
GFDIELAHKAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAI
EDGAAIIKQAQTWEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQR
QQVAPREPEPIKPDVPGWKDYISGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
DRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVMGNDEYKAMCGKLAAEHGFKITNPELQESIQQERQRIQQERVQAMKSEQLKQFERYAEAVGAERYRV
TSIKMREDGSKQTFILDKKDGITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYQPAAVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECIKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSL

Protein domains

Predicted by InterproScan.

• RepB DNA-primase from phage plasmid (RepB_primase) (548-807)

• Relaxase/Mobilisation nuclease domain (Relaxase) (45-274)

• Large polyvalent protein-associated domain 7 (LPD7) (439-527)

Protein structure

No available structure.

Host bacterium

• ID: 18291
• GenBank: NZ_AP022199
• Plasmid name: pWP7-S18-CRE-03_4
• Incompatibility group: IncQ2
• Plasmid size: 28179 bp
• Coordinate of oriT [Strand]: 12347..12483 [-]
• Host baterium: Klebsiella sp. WP7-S18-CRE-03

Cargo genes

• Drug resistance gene: tet(C)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -