Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   117832
Name   oriT_AUSMDU00004028|unnamed3 in_silico
Organism   Enterococcus faecium strain AUSMDU00004028
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP027515 (26610..26801 [-], 192 nt)
oriT length   192 nt
IRs (inverted repeats)      106..112, 122..128  (ATTTTTT..AAAAAAT)
 107..113, 120..126  (TTTTTTG..CAAAAAA)
 65..71, 75..81  (AGTTGGC..GCCAACT)
 41..49, 52..60  (CACCTTCCT..AGGAAGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 192 nt

>oriT_AUSMDU00004028|unnamed3
CCAAAGAATTAATGCAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTTTAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATCACTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11329 GenBank   WP_234384946
Name   mobP2_C6Y18_RS16160_AUSMDU00004028|unnamed3 insolico UniProt ID   _
Length   506 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 506 a.a.        Molecular weight: 60651.15 Da        Isoelectric Point: 9.4863

>WP_234384946.1 MobP2 family relaxase [Enterococcus faecium]
MTEHIFNRKSASIILKAKFETGKNKKKNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENILYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFNREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(11-437)


  Protein structure



No available structure.




T4CP

ID   13240 GenBank   WP_010776955
Name   t4cp2_C6Y18_RS16095_AUSMDU00004028|unnamed3 insolico UniProt ID   _
Length   952 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 952 a.a.        Molecular weight: 108268.15 Da        Isoelectric Point: 7.6503

>WP_010776955.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MKLVQNNEVKERKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(199-442)

TraM recognition site of TraD and TraG (TraG-D_C)

(589-701)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 7674..35351

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6Y18_RS16040 2955..6584 + 3630 WP_106913789 collagen binding domain-containing protein -
C6Y18_RS16045 6644..7006 + 363 WP_002338167 hypothetical protein -
C6Y18_RS16755 7020..7172 + 153 WP_002338168 hypothetical protein -
C6Y18_RS16050 7240..7662 + 423 WP_002338169 hypothetical protein -
C6Y18_RS16055 7674..9089 + 1416 WP_002338170 CpaF/VirB11 family protein virB11
C6Y18_RS16060 9106..9921 + 816 WP_080106305 hypothetical protein -
C6Y18_RS16065 9921..10718 + 798 WP_002338172 hypothetical protein -
C6Y18_RS16070 10736..11098 + 363 WP_002338173 hypothetical protein -
C6Y18_RS16760 11112..11258 + 147 WP_002326866 hypothetical protein -
C6Y18_RS16075 11270..11491 + 222 WP_002338174 hypothetical protein -
C6Y18_RS16080 11510..11785 + 276 WP_002311852 TrbC/VirB2 family protein virB2
C6Y18_RS16085 11894..13720 + 1827 WP_002338175 hypothetical protein -
C6Y18_RS16090 13733..14449 + 717 WP_106913790 hypothetical protein -
C6Y18_RS16095 14462..17320 + 2859 WP_010776955 VirD4-like conjugal transfer protein, CD1115 family -
C6Y18_RS16100 17338..20112 + 2775 WP_234384948 pLS20_p028 family conjugation system transmembrane protein -
C6Y18_RS16105 20160..20471 + 312 WP_002317413 DUF5592 family protein -
C6Y18_RS16110 20468..21085 + 618 WP_002311858 hypothetical protein virb4
C6Y18_RS16115 21102..21569 + 468 WP_002338147 hypothetical protein -
C6Y18_RS16120 21585..23540 + 1956 WP_002338148 VirB4 family type IV secretion system protein virb4
C6Y18_RS16125 23562..24698 + 1137 WP_002311861 bifunctional lytic transglycosylase/C40 family peptidase orf14
C6Y18_RS16130 24713..25363 + 651 WP_002338149 hypothetical protein -
C6Y18_RS16135 25377..26273 + 897 WP_002338150 hypothetical protein -
C6Y18_RS16140 26309..26539 + 231 WP_002311864 hypothetical protein -
C6Y18_RS16150 26890..27186 + 297 WP_010776936 hypothetical protein -
C6Y18_RS16155 27183..27650 + 468 WP_106913792 hypothetical protein -
C6Y18_RS16160 27733..29253 + 1521 WP_234384946 MobP2 family relaxase -
C6Y18_RS16165 29275..29604 + 330 WP_002338154 hypothetical protein -
C6Y18_RS16170 29606..29869 + 264 WP_002338155 hypothetical protein -
C6Y18_RS16175 30113..30979 + 867 WP_106913794 hypothetical protein -
C6Y18_RS16180 31134..32960 + 1827 WP_010776939 ArdC-like ssDNA-binding domain-containing protein -
C6Y18_RS16185 32968..34041 + 1074 WP_106913795 hypothetical protein -
C6Y18_RS16190 34050..34310 + 261 WP_002338160 hypothetical protein -
C6Y18_RS16195 34347..35351 + 1005 WP_002311875 DUF3991 domain-containing protein traP
C6Y18_RS16200 35376..35732 + 357 WP_106913796 hypothetical protein -
C6Y18_RS16205 35894..36085 + 192 WP_106913797 hypothetical protein -
C6Y18_RS16210 36194..36412 + 219 WP_002311878 hypothetical protein -
C6Y18_RS16215 36449..36766 + 318 WP_002338162 hypothetical protein -
C6Y18_RS16220 36786..36974 + 189 WP_002326871 hypothetical protein -
C6Y18_RS16225 36971..37939 + 969 WP_106913798 zeta toxin family protein -
C6Y18_RS16230 38372..38893 + 522 WP_010776944 helix-turn-helix domain-containing protein -
C6Y18_RS16235 39088..39984 + 897 WP_106913799 helix-turn-helix transcriptional regulator -

Host bacterium

ID   18265 GenBank   NZ_CP027515
Plasmid name   AUSMDU00004028|unnamed3 Incompatibility group   -
Plasmid size   53654 bp Coordinate of oriT [Strand]   26610..26801 [-]
Host baterium   Enterococcus faecium strain AUSMDU00004028

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1