Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 117831
• Name: oriT_AUSMDU00004024|unnamed2
• Organism: Enterococcus faecium strain AUSMDU00004024
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP027519 (39902..39924 [-], 23 nt)
• oriT length: 23 nt
• IRs (inverted repeats): _
• Location of nic site: 12..13
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 23 nt

>oriT_AUSMDU00004024|unnamed2
AAGTCTAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_C6Y19_RS16455_AUSMDU00004024|unnamed2

• ID: 11328
• GenBank: WP_002331393
• Name: Rep_trans_C6Y19_RS16455_AUSMDU00004024|unnamed2
• UniProt ID: A0A125W9H6
• Length: 312 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 312 a.a.
• Molecular weight: 37084.59 Da
• Isoelectric Point: 9.5266

>WP_002331393.1 MULTISPECIES: replication initiation factor domain-containing protein [Bacilli]
MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFDAMTIVGNLNKTNAKKLSDFMSVEPQI
RLWDILQTKFKAKALQEKVYIEYDKVKADTWDRRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLD
LAFDFEDDLSDYYAMTDKAVKKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWR
VEVELKRDMVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIREISPI
DLTELMKSTLKANEKQLQKQIDFWQREFRFWK

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (136-283)

Protein structure

• AlphaFold DB: A0A125W9H6

Host bacterium

• ID: 18264
• GenBank: NZ_CP027519
• Plasmid name: AUSMDU00004024|unnamed2
• Incompatibility group: -
• Plasmid size: 48209 bp
• Coordinate of oriT [Strand]: 39902..39924 [-]
• Host baterium: Enterococcus faecium strain AUSMDU00004024

Cargo genes

• Drug resistance gene: ant(6)-Ia, VanHAX, cat(pC221), erm(B)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -