Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   117763
Name   oriT_pPinheadLarry_01 in_silico
Organism   Klebsiella quasipneumoniae strain Pinhead Larry
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP065468 (11020..11108 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pPinheadLarry_01
GGGGTGTCGGGGCGAAGCCCTGATCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11291 GenBank   WP_011264053
Name   nikB_I5M56_RS24765_pPinheadLarry_01 insolico UniProt ID   A0A767TAZ8
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103882.34 Da        Isoelectric Point: 7.4198

>WP_011264053.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A767TAZ8


Auxiliary protein

ID   6515 GenBank   WP_001283947
Name   WP_001283947_pPinheadLarry_01 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   13184 GenBank   WP_011264051
Name   trbC_I5M56_RS24770_pPinheadLarry_01 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86954.10 Da        Isoelectric Point: 6.7713

>WP_011264051.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKNNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 16486..61088

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M56_RS24770 (I5M56_24770) 14202..16493 - 2292 WP_011264051 F-type conjugative transfer protein TrbC -
I5M56_RS24775 (I5M56_24775) 16486..17556 - 1071 WP_099010561 IncI1-type conjugal transfer protein TrbB trbB
I5M56_RS24780 (I5M56_24780) 17575..18783 - 1209 WP_011264049 IncI1-type conjugal transfer protein TrbA trbA
I5M56_RS24785 (I5M56_24785) 19090..19872 - 783 WP_023063753 protein FinQ -
I5M56_RS24790 (I5M56_24790) 20068..20385 + 318 WP_000118520 quaternary ammonium compound efflux SMR transporter SugE -
I5M56_RS24795 (I5M56_24795) 20382..20915 - 534 WP_001221666 lipocalin family protein -
I5M56_RS24800 (I5M56_24800) 21009..22154 - 1146 WP_000976514 extended-spectrum class C beta-lactamase CMY-2 -
I5M56_RS24805 (I5M56_24805) 22478..22813 - 336 Protein_24 transposase -
I5M56_RS24810 (I5M56_24810) 22872..23840 - 969 WP_020219382 IS5 family transposase -
I5M56_RS24815 (I5M56_24815) 23874..24806 - 933 Protein_26 IS1380-like element ISEcp1 family transposase -
I5M56_RS24820 (I5M56_24820) 24959..25333 - 375 WP_223349261 hypothetical protein -
I5M56_RS24825 (I5M56_24825) 25654..25806 + 153 WP_001387489 Hok/Gef family protein -
I5M56_RS24830 (I5M56_24830) 25878..26129 - 252 WP_001291964 hypothetical protein -
I5M56_RS26875 26630..26725 + 96 WP_000609148 DinQ-like type I toxin DqlB -
I5M56_RS24840 (I5M56_24840) 26790..26966 - 177 WP_001054897 hypothetical protein -
I5M56_RS24845 (I5M56_24845) 27358..27567 + 210 WP_000062603 HEAT repeat domain-containing protein -
I5M56_RS24850 (I5M56_24850) 27639..28301 - 663 WP_000644796 plasmid IncI1-type surface exclusion protein ExcA -
I5M56_RS24855 (I5M56_24855) 28372..30540 - 2169 WP_023518847 DotA/TraY family protein traY
I5M56_RS24860 (I5M56_24860) 30637..31221 - 585 WP_001746157 IncI1-type conjugal transfer protein TraX -
I5M56_RS24865 (I5M56_24865) 31250..32452 - 1203 WP_001189160 IncI1-type conjugal transfer protein TraW traW
I5M56_RS24870 (I5M56_24870) 32419..33033 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
I5M56_RS24875 (I5M56_24875) 33033..36077 - 3045 WP_194425974 IncI1-type conjugal transfer protein TraU traU
I5M56_RS24880 (I5M56_24880) 36167..36967 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
I5M56_RS24885 (I5M56_24885) 36951..37139 - 189 WP_001277255 putative conjugal transfer protein TraS -
I5M56_RS24890 (I5M56_24890) 37203..37607 - 405 WP_000086965 IncI1-type conjugal transfer protein TraR traR
I5M56_RS24895 (I5M56_24895) 37658..38185 - 528 WP_001055569 conjugal transfer protein TraQ traQ
I5M56_RS24900 (I5M56_24900) 38185..38889 - 705 WP_001493811 IncI1-type conjugal transfer protein TraP traP
I5M56_RS24905 (I5M56_24905) 38889..40178 - 1290 WP_011264042 conjugal transfer protein TraO traO
I5M56_RS24910 (I5M56_24910) 40181..41164 - 984 WP_001191880 IncI1-type conjugal transfer protein TraN traN
I5M56_RS24915 (I5M56_24915) 41175..41867 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
I5M56_RS24920 (I5M56_24920) 41864..42211 - 348 WP_001055900 conjugal transfer protein traL
I5M56_RS24925 (I5M56_24925) 42229..45996 - 3768 WP_194425975 LPD7 domain-containing protein -
I5M56_RS24930 (I5M56_24930) 46086..46637 - 552 WP_000014583 phospholipase D family protein -
I5M56_RS24935 (I5M56_24935) 46652..46942 - 291 WP_001299214 hypothetical protein traK
I5M56_RS24940 (I5M56_24940) 46939..48087 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
I5M56_RS24945 (I5M56_24945) 48084..48902 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
I5M56_RS24950 (I5M56_24950) 48899..49357 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
I5M56_RS24955 (I5M56_24955) 49752..50336 - 585 WP_000977522 histidine phosphatase family protein -
I5M56_RS24960 (I5M56_24960) 50396..51598 - 1203 WP_000976351 conjugal transfer protein TraF -
I5M56_RS24965 (I5M56_24965) 51683..52507 - 825 WP_001238939 conjugal transfer protein TraE traE
I5M56_RS24970 (I5M56_24970) 52658..53812 - 1155 WP_001139957 tyrosine-type recombinase/integrase -
I5M56_RS26645 53811..54095 + 285 WP_187642572 pilus assembly protein PilV -
I5M56_RS24980 (I5M56_24980) 55281..56582 - 1302 Protein_59 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
I5M56_RS24985 (I5M56_24985) 56582..57238 - 657 WP_001193553 prepilin peptidase -
I5M56_RS24990 (I5M56_24990) 57223..57783 - 561 WP_000014116 lytic transglycosylase domain-containing protein virB1
I5M56_RS24995 (I5M56_24995) 57793..58407 - 615 WP_000959785 type 4 pilus major pilin -
I5M56_RS25000 (I5M56_25000) 58425..59522 - 1098 WP_001208805 type II secretion system F family protein -
I5M56_RS25005 (I5M56_25005) 59535..61088 - 1554 WP_000362202 GspE/PulE family protein virB11
I5M56_RS25010 (I5M56_25010) 61099..61551 - 453 WP_001247334 type IV pilus biogenesis protein PilP -
I5M56_RS25015 (I5M56_25015) 61538..62833 - 1296 WP_000752772 type 4b pilus protein PilO2 -
I5M56_RS25020 (I5M56_25020) 62826..64508 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
I5M56_RS25025 (I5M56_25025) 64522..64959 - 438 WP_000539807 type IV pilus biogenesis protein PilM -
I5M56_RS25030 (I5M56_25030) 64959..66026 - 1068 WP_001302629 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   18196 GenBank   NZ_CP065468
Plasmid name   pPinheadLarry_01 Incompatibility group   IncI1
Plasmid size   76488 bp Coordinate of oriT [Strand]   11020..11108 [-]
Host baterium   Klebsiella quasipneumoniae strain Pinhead Larry

Cargo genes

Drug resistance gene   blaCMY-2, sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2