Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 117675 |
| Name | oriT_pBS326-1  |
| Organism | Klebsiella variicola strain BS326-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP063899 (82807..82856 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pBS326-1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11248 | GenBank | WP_153669800 |
| Name | traI_ISA87_RS27025_pBS326-1 |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190780.74 Da Isoelectric Point: 6.0386
>WP_153669800.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKTLFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQTLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVCEGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITSLTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEQAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VERVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKTLFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQTLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVCEGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITSLTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEQAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VERVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 13135 | GenBank | WP_047665855 |
| Name | traD_ISA87_RS27020_pBS326-1 |
UniProt ID | _ |
| Length | 768 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 768 a.a. Molecular weight: 85847.91 Da Isoelectric Point: 5.0689
>WP_047665855.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSQPA
PAEVTVSPAPVKAPPTTKSPAAEPSARAAEPPVLRVTTVPLIKPKAAAAAAAAATASSVGTPAAGGTEQE
LAQQSAEQGQEMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINRSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSQPA
PAEVTVSPAPVKAPPTTKSPAAEPSARAAEPPVLRVTTVPLIKPKAAAAAAAAATASSVGTPAAGGTEQE
LAQQSAEQGQEMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINRSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 85354..111301
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ISA87_RS26835 | 81687..82217 | + | 531 | WP_040107921 | antirestriction protein | - |
| ISA87_RS26840 | 82255..82662 | - | 408 | WP_223170089 | transglycosylase SLT domain-containing protein | - |
| ISA87_RS26845 | 83169..83561 | + | 393 | WP_047665760 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| ISA87_RS26850 | 83768..84463 | + | 696 | WP_072194100 | transcriptional regulator TraJ family protein | - |
| ISA87_RS26855 | 84547..84918 | + | 372 | WP_071885111 | TraY domain-containing protein | - |
| ISA87_RS26860 | 84972..85340 | + | 369 | WP_047665762 | type IV conjugative transfer system pilin TraA | - |
| ISA87_RS26865 | 85354..85659 | + | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| ISA87_RS26870 | 85679..86245 | + | 567 | WP_047665763 | type IV conjugative transfer system protein TraE | traE |
| ISA87_RS26875 | 86232..86972 | + | 741 | WP_013214019 | type-F conjugative transfer system secretin TraK | traK |
| ISA87_RS26880 | 86972..88396 | + | 1425 | WP_046622826 | F-type conjugal transfer pilus assembly protein TraB | traB |
| ISA87_RS26885 | 88389..88985 | + | 597 | WP_047665764 | conjugal transfer pilus-stabilizing protein TraP | - |
| ISA87_RS26890 | 89008..89577 | + | 570 | WP_046622829 | type IV conjugative transfer system lipoprotein TraV | traV |
| ISA87_RS27375 | 89733..90131 | + | 399 | WP_223170083 | hypothetical protein | - |
| ISA87_RS26900 | 90160..90450 | + | 291 | WP_047665765 | hypothetical protein | - |
| ISA87_RS26905 | 90474..90692 | + | 219 | WP_014386199 | hypothetical protein | - |
| ISA87_RS26910 | 90693..91004 | + | 312 | WP_047665766 | hypothetical protein | - |
| ISA87_RS26915 | 91071..91475 | + | 405 | WP_046622833 | hypothetical protein | - |
| ISA87_RS27380 | 91667..91909 | + | 243 | WP_071885119 | hypothetical protein | - |
| ISA87_RS26925 | 91917..92315 | + | 399 | WP_004153071 | hypothetical protein | - |
| ISA87_RS26930 | 92387..95026 | + | 2640 | WP_046622834 | type IV secretion system protein TraC | virb4 |
| ISA87_RS26935 | 95026..95415 | + | 390 | WP_046622835 | type-F conjugative transfer system protein TrbI | - |
| ISA87_RS26940 | 95415..96041 | + | 627 | WP_004152507 | type-F conjugative transfer system protein TraW | traW |
| ISA87_RS26945 | 96083..96472 | + | 390 | WP_047667530 | hypothetical protein | - |
| ISA87_RS26950 | 96469..97458 | + | 990 | WP_046622837 | conjugal transfer pilus assembly protein TraU | traU |
| ISA87_RS26955 | 97471..98109 | + | 639 | WP_047667531 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| ISA87_RS26960 | 98168..100123 | + | 1956 | WP_131011026 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| ISA87_RS26965 | 100156..100437 | + | 282 | WP_047665844 | hypothetical protein | - |
| ISA87_RS26970 | 100427..100654 | + | 228 | WP_047665846 | conjugal transfer protein TrbE | - |
| ISA87_RS26975 | 100665..100991 | + | 327 | WP_012539967 | hypothetical protein | - |
| ISA87_RS26980 | 101012..101764 | + | 753 | WP_047665849 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| ISA87_RS26985 | 101775..102014 | + | 240 | WP_009309875 | type-F conjugative transfer system pilin chaperone TraQ | - |
| ISA87_RS26990 | 101986..102558 | + | 573 | WP_055323574 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| ISA87_RS26995 | 102551..102973 | + | 423 | WP_049010971 | hypothetical protein | - |
| ISA87_RS27000 | 102960..104330 | + | 1371 | WP_047665852 | conjugal transfer pilus assembly protein TraH | traH |
| ISA87_RS27005 | 104330..107179 | + | 2850 | WP_047665853 | conjugal transfer mating-pair stabilization protein TraG | traG |
| ISA87_RS27010 | 107185..107712 | + | 528 | WP_047665858 | conjugal transfer protein TraS | - |
| ISA87_RS27015 | 107902..108633 | + | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| ISA87_RS27385 | 108826..108921 | + | 96 | Protein_124 | hypothetical protein | - |
| ISA87_RS27020 | 108995..111301 | + | 2307 | WP_047665855 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 18108 | GenBank | NZ_CP063899 |
| Plasmid name | pBS326-1 | Incompatibility group | IncFIB |
| Plasmid size | 139497 bp | Coordinate of oriT [Strand] | 82807..82856 [-] |
| Host baterium | Klebsiella variicola strain BS326-1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsR, arsD, arsA, arsB, arsC, arsH |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |