Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 117594
• Name: oriT_pWP3-S18-ESBL-03_4
• Organism: Klebsiella quasipneumoniae strain WP3-S18-ESBL-03
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP022018 (70277..70325 [-], 49 nt)
• oriT length: 49 nt
• IRs (inverted repeats): 6..13, 16..23 (GCAAAATT..AATTTTGC)
• Location of nic site: 32..33
• Conserved sequence flanking the nic site: TGTGTGGTGA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 49 nt

>oriT_pWP3-S18-ESBL-03_4
AATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_H7R87_RS29775_pWP3-S18-ESBL-03_4

• ID: 11215
• GenBank: WP_182972118
• Name: traI_H7R87_RS29775_pWP3-S18-ESBL-03_4
• UniProt ID: _
• Length: 1752 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1752 a.a.
• Molecular weight: 190096.40 Da
• Isoelectric Point: 5.9146

>WP_182972118.1 conjugative transfer relaxase/helicase TraI [Klebsiella quasipneumoniae]
MLSFSQVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKRDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSENILANRIALGKIYQNSLRADVES
MGYKTVDAGKNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDASVVREAPF
SDAVSVLAQDRPAMGIVAGQGGAAGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGKQATADIISEPDKSARYSRLAQEFAASVREGQESVAQISGTREQNVLNNVIRDTLKNEGALGNRDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRKHDRFVIDRVTASSNMLTLKDREGERLDLKVSAVD
SQWTLFRTDKLPVAEGERLAVLGKIPDTRLKGGESVTVLKAEAGQLTVQRPGQKTTQTLPVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQDAARTTEKLARHTAFSVVSEQ
LKARSGETDLEEAIAQQKAGLHTPAEQAIHLSVPLLESNSLTFTRPQLLATALETGGGKVPMKDIEATIQ
AQIKAGSLLNVPVASGHGNDLLISRQTWDAEKSVLTRVLEGKDAVAPLMGRVPGSLMTDLTAGQRAATRM
ILETPDRFTVVQGYAGVGKTTQFKAVMSAISLLPEETRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLQQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVSRALSTIEQVTPEQVPRKDGAWVPENSVTEFTPAQEKAIQD
ALKEGKEIPPDTPASLHDAIVKDYTGRTPEAQANTLIITHLNADRRALNGLIHDARVQNGEVSKEEVTLP
VLVTSNIRDGELRKLSTWSEHQGAVALLDNIYQRIESVDKENQLITLKDNEGNIRLLSPREASAEGVTLY
RPEHITVSQGDRMRFSKSDPERGYVANSVWEVKAVSGDSVTLSGGKTTRTLNPKADEAQRHVDLAYAITA
HGAQGASEPFAIALEGVEGRRKALVSFESAYVALSRMKQHAQVYTDNREGWTTAVSRSQEKASAHDVLEP
QNARAVKNAGQLYGRAQPLDETAAGRAALQQSGLARGRSPGKFISPGKKYPQPHVALPAFDKNGKEAGIW
LSPLTDRDGRLEAIGGEGRIMGDETARFVALQNSRNGESLLAGNMGEGVRTARDHPDSGVIVRLAGDDRP
WNPEAITGGRIWADPLPNPPLPDTGTDIPLPPEVLAQRAAEEAQRREMEKQAEQATREVAGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTLLPDDPQTRRQEAAIQQVASESLQRERLQQMERDMVRDLNREKTLG
GD

Protein domains

Predicted by InterproScan.

• TrwC relaxase (TrwC) (10-283)

• single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N) (576-628)

• DNA relaxase TraI 2B/2B-like domain (TraI_2B) (634-713)

• DNA helicase TraI (TraI_C) (1432-1554)

• AAA domain (AAA_30) (970-1157)

Protein structure

No available structure.

Auxiliary protein

WP_032426796_pWP3-S18-ESBL-03_4

• ID: 6484
• GenBank: WP_032426796
• Name: WP_032426796_pWP3-S18-ESBL-03_4
• UniProt ID: _
• Length: 130 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 130 a.a.
• Molecular weight: 15036.10 Da
• Isoelectric Point: 4.5326

>WP_032426796.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacterales]
MGKVQAYPSDEVCEKINAIVEKRRMEGAKEKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNRAL
LESVVKTQFSVNKVLGIECLSPHVKDDPRWQWKGMVQNIQEDVQEVMRTFFPDEQEEADE

Protein domains

Predicted by InterproScan.

• TraM protein, DNA-binding (Tra_M) (1-125)

Protein structure

No available structure.

T4CP

traD_H7R87_RS29770_pWP3-S18-ESBL-03_4

• ID: 13099
• GenBank: WP_020803851
• Name: traD_H7R87_RS29770_pWP3-S18-ESBL-03_4
• UniProt ID: _
• Length: 770 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 770 a.a.
• Molecular weight: 85873.87 Da
• Isoelectric Point: 4.9596

>WP_020803851.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFIFAAVVSLVICIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLSLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAM
KYKPRPKIAEGFIQRTPDTRADARLSALLEAREAEGSLARALFTPDAPELAGKDSTPGEQPEPVSQPAPA
EVAVSPAPVNAPPTTKSPAAEPSAGAPASPPPEATVLRNTTVPLIKPKAAGTAATASSAGTPVAPAGGTE
QELVQHSTEQGRDMLPAGMNEDGVIEDMQAYDAWLADEQTLRDMQRREEVNINHSHRHDEQDDVEIGGNF

Protein domains

Predicted by InterproScan.

• F sex factor protein N terminal (TraD_N) (32-128)

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (172-560)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 72744..98842

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R87_RS29575 (WP3S18E03_P40820)	67953..68183	+	231	WP_020314756	hypothetical protein	-
H7R87_RS29580 (WP3S18E03_P40830)	68281..68496	+	216	Protein_91	SAM-dependent DNA methyltransferase	-
H7R87_RS29585 (WP3S18E03_P40840)	69145..69687	+	543	WP_032426797	antirestriction protein	-
H7R87_RS29590 (WP3S18E03_P40850)	69710..70132	-	423	WP_020315406	transglycosylase SLT domain-containing protein	-
H7R87_RS29595 (WP3S18E03_P40860)	70638..71030	+	393	WP_032426796	conjugal transfer relaxosome DNA-binding protein TraM	-
H7R87_RS29600 (WP3S18E03_P40870)	71268..71960	+	693	WP_032426795	conjugal transfer protein TrbJ	-
H7R87_RS29605	72136..72300	+	165	WP_032444506	TraY domain-containing protein	-
H7R87_RS29610 (WP3S18E03_P40880)	72362..72730	+	369	WP_087829211	type IV conjugative transfer system pilin TraA	-
H7R87_RS29615 (WP3S18E03_P40890)	72744..73049	+	306	WP_087829212	type IV conjugative transfer system protein TraL	traL
H7R87_RS29620 (WP3S18E03_P40900)	73069..73635	+	567	WP_064373744	type IV conjugative transfer system protein TraE	traE
H7R87_RS29625 (WP3S18E03_P40910)	73622..74362	+	741	WP_020804840	type-F conjugative transfer system secretin TraK	traK
H7R87_RS29630 (WP3S18E03_P40920)	74362..75786	+	1425	WP_182949619	F-type conjugal transfer pilus assembly protein TraB	traB
H7R87_RS29635 (WP3S18E03_P40930)	75900..76469	+	570	WP_182949616	type IV conjugative transfer system lipoprotein TraV	traV
H7R87_RS30360	76625..77023	+	399	WP_223177007	hypothetical protein	-
H7R87_RS29645 (WP3S18E03_P40940)	77052..77342	+	291	WP_032426790	hypothetical protein	-
H7R87_RS29650 (WP3S18E03_P40950)	77366..77584	+	219	WP_023316402	hypothetical protein	-
H7R87_RS29655 (WP3S18E03_P40960)	77585..77896	+	312	WP_023316403	hypothetical protein	-
H7R87_RS29660 (WP3S18E03_P40970)	77963..78367	+	405	WP_023316404	hypothetical protein	-
H7R87_RS29665 (WP3S18E03_P40980)	78364..78654	+	291	WP_020323516	hypothetical protein	-
H7R87_RS29670 (WP3S18E03_P40990)	78662..79060	+	399	WP_072156904	hypothetical protein	-
H7R87_RS29675 (WP3S18E03_P41000)	79132..81771	+	2640	WP_020323518	type IV secretion system protein TraC	virb4
H7R87_RS29680 (WP3S18E03_P41010)	81771..82160	+	390	WP_020314627	type-F conjugative transfer system protein TrbI	-
H7R87_RS29685 (WP3S18E03_P41020)	82160..82786	+	627	WP_020314628	type-F conjugative transfer system protein TraW	traW
H7R87_RS29690 (WP3S18E03_P41030)	82830..83789	+	960	WP_029497356	conjugal transfer pilus assembly protein TraU	traU
H7R87_RS29695 (WP3S18E03_P41040)	83802..84440	+	639	WP_064182677	type-F conjugative transfer system pilin assembly protein TrbC	trbC
H7R87_RS29700 (WP3S18E03_P41050)	84499..86454	+	1956	WP_182949614	type-F conjugative transfer system mating-pair stabilization protein TraN	traN
H7R87_RS29705 (WP3S18E03_P41060)	86487..86768	+	282	WP_032419932	hypothetical protein	-
H7R87_RS29710 (WP3S18E03_P41070)	86758..86985	+	228	WP_012540032	conjugal transfer protein TrbE	-
H7R87_RS29715 (WP3S18E03_P41080)	86996..87322	+	327	WP_020323521	hypothetical protein	-
H7R87_RS29720 (WP3S18E03_P41090)	87343..88095	+	753	WP_136071131	type-F conjugative transfer system pilin assembly protein TraF	traF
H7R87_RS29725 (WP3S18E03_P41100)	88550..88789	+	240	WP_023316407	type-F conjugative transfer system pilin chaperone TraQ	-
H7R87_RS29730 (WP3S18E03_P41110)	88761..89318	+	558	WP_182972117	type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB	traF
H7R87_RS29735	89406..90103	+	698	WP_227666874	IS1 family transposase	-
H7R87_RS29740 (WP3S18E03_P41130)	90141..90584	+	444	WP_020804675	F-type conjugal transfer protein TrbF	-
H7R87_RS29745 (WP3S18E03_P41140)	90562..91941	+	1380	WP_162495503	conjugal transfer pilus assembly protein TraH	traH
H7R87_RS29750 (WP3S18E03_P41150)	91941..94790	+	2850	WP_032744228	conjugal transfer mating-pair stabilization protein TraG	traG
H7R87_RS29755 (WP3S18E03_P41160)	94796..95323	+	528	WP_020803849	hypothetical protein	-
H7R87_RS30365 (WP3S18E03_P41170)	95506..96174	+	669	WP_020803846	hypothetical protein	-
H7R87_RS29765	96198..96458	+	261	WP_020803850	hypothetical protein	-
H7R87_RS29770 (WP3S18E03_P41180)	96530..98842	+	2313	WP_020803851	type IV conjugative transfer system coupling protein TraD	virb4

Host bacterium

• ID: 18027
• GenBank: NZ_AP022018
• Plasmid name: pWP3-S18-ESBL-03_4
• Incompatibility group: -
• Plasmid size: 111708 bp
• Coordinate of oriT [Strand]: 70277..70325 [-]
• Host baterium: Klebsiella quasipneumoniae strain WP3-S18-ESBL-03

Cargo genes

• Drug resistance gene: blaGES-4, catB8, aac(6')-31, qacE, sul1
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -