Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   117585
Name   oriT_pWP5-W18-ESBL-06_2 in_silico
Organism   Klebsiella quasipneumoniae strain WP5-W18-ESBL-06
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP022114 (39885..39933 [+], 49 nt)
oriT length   49 nt
IRs (inverted repeats)      6..13, 16..23  (GCAAAATT..AATTTTGC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 49 nt

>oriT_pWP5-W18-ESBL-06_2
AATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11207 GenBank   WP_182937536
Name   traI_H7R83_RS27720_pWP5-W18-ESBL-06_2 insolico UniProt ID   _
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190398.15 Da        Isoelectric Point: 6.1727

>WP_182937536.1 conjugative transfer relaxase/helicase TraI [Klebsiella quasipneumoniae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDGGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIAAGGGRAVPSGDTDQLPPIASGQPFRLTQQRS
AADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

TrwC relaxase (TrwC)

(11-283)

AAA domain (AAA_30)

(970-1156)

DNA helicase TraI (TraI_C)

(1432-1554)


  Protein structure



No available structure.




Auxiliary protein

ID   6481 GenBank   WP_004197733
Name   WP_004197733_pWP5-W18-ESBL-06_2 insolico UniProt ID   A0A0E3KB00
Length   129 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 129 a.a.        Molecular weight: 14835.90 Da        Isoelectric Point: 4.6012

>WP_004197733.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Bacteria]
MGKVQAYPSDEVCEKINAIVEKRRMEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNRAL
LESVVKTQFSVNKILGIECLSPHVKDDPRWQWKSMVLNIQEDVQEAMRNFFPDEDAEGE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-124)


  Protein structure


Source ID Structure
AlphaFold DB A0A0E3KB00


T4CP

ID   13090 GenBank   WP_013214038
Name   traD_H7R83_RS27725_pWP5-W18-ESBL-06_2 insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 86010.07 Da        Isoelectric Point: 5.1787

>WP_013214038.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTIKRPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 11498..40485

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R83_RS27725 (WP5W18E06_P20100) 11498..13810 - 2313 WP_013214038 type IV conjugative transfer system coupling protein TraD virb4
H7R83_RS27730 (WP5W18E06_P20110) 13937..14626 - 690 WP_162497697 hypothetical protein -
H7R83_RS27735 (WP5W18E06_P20120) 14819..15550 - 732 WP_004152629 conjugal transfer complement resistance protein TraT -
H7R83_RS27740 (WP5W18E06_P20130) 15859..16383 - 525 WP_040206919 hypothetical protein -
H7R83_RS27745 (WP5W18E06_P20140) 16394..19237 - 2844 WP_094833276 conjugal transfer mating-pair stabilization protein TraG traG
H7R83_RS27750 (WP5W18E06_P20150) 19237..20616 - 1380 WP_075253037 conjugal transfer pilus assembly protein TraH traH
H7R83_RS27755 (WP5W18E06_P20160) 20594..21037 - 444 WP_023287131 F-type conjugal transfer protein TrbF -
H7R83_RS27760 (WP5W18E06_P20170) 21083..21640 - 558 WP_013214031 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
H7R83_RS27765 (WP5W18E06_P20180) 21612..21851 - 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
H7R83_RS27770 (WP5W18E06_P20190) 21862..22614 - 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
H7R83_RS27775 (WP5W18E06_P20200) 22635..22961 - 327 WP_004152676 hypothetical protein -
H7R83_RS27780 (WP5W18E06_P20210) 22974..23222 - 249 WP_013214029 hypothetical protein -
H7R83_RS27785 (WP5W18E06_P20220) 23200..23454 - 255 WP_023287133 conjugal transfer protein TrbE -
H7R83_RS27790 (WP5W18E06_P20230) 23486..25441 - 1956 WP_182937538 type-F conjugative transfer system mating-pair stabilization protein TraN traN
H7R83_RS27795 (WP5W18E06_P20240) 25500..26138 - 639 WP_004193871 type-F conjugative transfer system pilin assembly protein TrbC trbC
H7R83_RS27800 (WP5W18E06_P20250) 26151..27140 - 990 WP_032441886 conjugal transfer pilus assembly protein TraU traU
H7R83_RS27805 (WP5W18E06_P20260) 27137..27538 - 402 WP_004194979 hypothetical protein -
H7R83_RS27810 (WP5W18E06_P20270) 27573..28208 - 636 WP_032441885 type-F conjugative transfer system protein TraW traW
H7R83_RS27815 (WP5W18E06_P20280) 28208..28597 - 390 WP_004167468 type-F conjugative transfer system protein TrbI -
H7R83_RS27820 (WP5W18E06_P20300) 28594..31235 - 2642 Protein_30 type IV secretion system protein TraC -
H7R83_RS27825 (WP5W18E06_P20310) 31307..31705 - 399 WP_023179972 hypothetical protein -
H7R83_RS27830 (WP5W18E06_P20320) 32082..32486 - 405 WP_182937540 hypothetical protein -
H7R83_RS27835 (WP5W18E06_P20330) 32553..32864 - 312 WP_004195465 hypothetical protein -
H7R83_RS27840 (WP5W18E06_P20340) 32865..33083 - 219 WP_004195468 hypothetical protein -
H7R83_RS27845 (WP5W18E06_P20350) 33189..33599 - 411 WP_009309869 hypothetical protein -
H7R83_RS27850 (WP5W18E06_P20360) 33731..34315 - 585 WP_009309868 type IV conjugative transfer system lipoprotein TraV traV
H7R83_RS27855 (WP5W18E06_P20370) 34429..35853 - 1425 WP_004194260 F-type conjugal transfer pilus assembly protein TraB traB
H7R83_RS27860 (WP5W18E06_P20380) 35853..36593 - 741 WP_009309865 type-F conjugative transfer system secretin TraK traK
H7R83_RS27865 (WP5W18E06_P20390) 36580..37146 - 567 WP_004194424 type IV conjugative transfer system protein TraE traE
H7R83_RS27870 (WP5W18E06_P20400) 37166..37471 - 306 WP_004178059 type IV conjugative transfer system protein TraL traL
H7R83_RS27875 (WP5W18E06_P20410) 37485..37853 - 369 WP_004194426 type IV conjugative transfer system pilin TraA -
H7R83_RS27880 37915..38079 - 165 WP_032444506 TraY domain-containing protein -
H7R83_RS27885 (WP5W18E06_P20420) 38271..38945 - 675 WP_004197737 hypothetical protein -
H7R83_RS27890 (WP5W18E06_P20430) 39184..39573 - 390 WP_004197733 conjugal transfer relaxosome DNA-binding protein TraM -
H7R83_RS27895 (WP5W18E06_P20440) 40078..40485 + 408 WP_009309864 transglycosylase SLT domain-containing protein virB1
H7R83_RS27900 (WP5W18E06_P20450) 40523..41053 - 531 WP_004197734 antirestriction protein -
H7R83_RS27905 (WP5W18E06_P20460) 41678..42511 - 834 WP_004197739 N-6 DNA methylase -
H7R83_RS27910 (WP5W18E06_P20470) 42558..42788 - 231 WP_004197732 hypothetical protein -
H7R83_RS27915 (WP5W18E06_P20480) 42876..43223 - 348 WP_004197735 hypothetical protein -
H7R83_RS27920 (WP5W18E06_P20490) 43290..43670 - 381 WP_004197740 hypothetical protein -
H7R83_RS27925 (WP5W18E06_P20500) 44678..44839 - 162 WP_004118124 type I toxin-antitoxin system Hok family toxin -

Host bacterium

ID   18018 GenBank   NZ_AP022114
Plasmid name   pWP5-W18-ESBL-06_2 Incompatibility group   IncFII
Plasmid size   120877 bp Coordinate of oriT [Strand]   39885..39933 [+]
Host baterium   Klebsiella quasipneumoniae strain WP5-W18-ESBL-06

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -