Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 117584 |
| Name | oriT_pKp4116  |
| Organism | Klebsiella quasipneumoniae strain N18-04116 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP047284 (31805..32411 [+], 607 nt) |
| oriT length | 607 nt |
| IRs (inverted repeats) | 590..595, 600..605 (ATGCCC..GGGCAT) 517..523, 529..535 (CCTCCCG..CGGGAGG) 453..458, 462..467 (GTTCGC..GCGAAC) 196..202, 204..210 (TCAATTC..GAATTGA) 175..180, 187..192 (AAATCA..TGATTT) 103..108, 120..125 (GCTAAA..TTTAGC) 79..84, 93..98 (GCTTTA..TAAAGC) 29..37, 46..54 (TTTTGATAA..TTATCAAAA) |
| Location of nic site | 313..314 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 607 nt
>oriT_pKp4116
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCAGTGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCAGTGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11206 | GenBank | WP_001576909 |
| Name | mobP1_F3137_RS25525_pKp4116 |
UniProt ID | A0A631M5V6 |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44446.83 Da Isoelectric Point: 10.5067
>WP_001576909.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFLGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A631M5V6 |
T4CP
| ID | 13089 | GenBank | WP_000053829 |
| Name | t4cp2_F3137_RS25460_pKp4116 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69290.20 Da Isoelectric Point: 6.9121
>WP_000053829.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWVFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKNFDSMLLWKIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFVIWQLNKMDVALYGDAKFASDNDLKKSKLLKWETENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSASQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWVFIGLVMCLVTYYAGSVAVYFLNGKTPLYIWKNFDSMLLWKIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFVIWQLNKMDVALYGDAKFASDNDLKKSKLLKWETENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSASQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 19305..29265
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| F3137_RS25425 (F3137_25245) | 14646..14966 | - | 321 | WP_001020837 | hypothetical protein | - |
| F3137_RS25430 (F3137_25250) | 15026..15430 | - | 405 | WP_001576899 | hypothetical protein | - |
| F3137_RS25435 (F3137_25255) | 15446..15982 | - | 537 | WP_001576898 | thermonuclease family protein | - |
| F3137_RS25440 | 15979..16413 | - | 435 | WP_001547186 | hypothetical protein | - |
| F3137_RS25445 (F3137_25265) | 16495..16752 | - | 258 | WP_001547185 | hypothetical protein | - |
| F3137_RS25450 (F3137_25270) | 16758..17060 | - | 303 | WP_001576896 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| F3137_RS25455 (F3137_25275) | 17057..17470 | - | 414 | WP_001579347 | cag pathogenicity island Cag12 family protein | - |
| F3137_RS25460 (F3137_25280) | 17467..19302 | - | 1836 | WP_000053829 | type IV secretory system conjugative DNA transfer family protein | - |
| F3137_RS25465 (F3137_25285) | 19305..20336 | - | 1032 | WP_001058476 | P-type DNA transfer ATPase VirB11 | virB11 |
| F3137_RS25470 (F3137_25290) | 20338..21543 | - | 1206 | WP_001547183 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| F3137_RS25475 (F3137_25295) | 21540..22469 | - | 930 | WP_000783381 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| F3137_RS25480 (F3137_25300) | 22474..23187 | - | 714 | WP_000394613 | type IV secretion system protein | virB8 |
| F3137_RS25485 (F3137_25305) | 23432..24565 | - | 1134 | WP_001576910 | type IV secretion system protein | virB6 |
| F3137_RS25490 (F3137_25310) | 24575..24802 | - | 228 | WP_000835348 | EexN family lipoprotein | - |
| F3137_RS25495 (F3137_25315) | 24803..25561 | - | 759 | WP_000744201 | type IV secretion system protein | - |
| F3137_RS25500 (F3137_25320) | 25572..28325 | - | 2754 | WP_001363160 | VirB3 family type IV secretion system protein | virb4 |
| F3137_RS25505 (F3137_25325) | 28344..28640 | - | 297 | WP_000040716 | TrbC/VirB2 family protein | virB2 |
| F3137_RS25510 (F3137_25330) | 28618..29265 | - | 648 | WP_000953535 | lytic transglycosylase domain-containing protein | virB1 |
| F3137_RS25515 (F3137_25335) | 29298..29510 | - | 213 | WP_000125172 | hypothetical protein | - |
| F3137_RS25520 (F3137_25340) | 29440..29958 | - | 519 | WP_001446885 | transcription termination/antitermination NusG family protein | - |
| F3137_RS25525 (F3137_25345) | 30311..31477 | - | 1167 | WP_001576909 | MobP1 family relaxase | - |
| F3137_RS25530 (F3137_25350) | 31480..32025 | - | 546 | WP_000757693 | DNA distortion polypeptide 1 | - |
| F3137_RS25540 (F3137_25355) | 32351..32623 | - | 273 | WP_000160399 | hypothetical protein | - |
| F3137_RS25545 | 32636..32785 | - | 150 | WP_000003880 | hypothetical protein | - |
| F3137_RS25550 (F3137_25360) | 32855..33034 | - | 180 | WP_000439078 | hypothetical protein | - |
| F3137_RS25555 (F3137_25365) | 33077..33406 | - | 330 | WP_000866648 | hypothetical protein | - |
| F3137_RS25560 (F3137_25370) | 33499..33714 | - | 216 | WP_001180116 | hypothetical protein | - |
| F3137_RS25565 (F3137_25375) | 33704..33949 | - | 246 | WP_000356546 | hypothetical protein | - |
Host bacterium
| ID | 18017 | GenBank | NZ_CP047284 |
| Plasmid name | pKp4116 | Incompatibility group | IncX1 |
| Plasmid size | 39531 bp | Coordinate of oriT [Strand] | 31805..32411 [+] |
| Host baterium | Klebsiella quasipneumoniae strain N18-04116 |
Cargo genes
| Drug resistance gene | blaOXA-204 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |