Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   117572
Name   oriT_pWP3-S18-CRE-02_5 in_silico
Organism   Klebsiella quasipneumoniae strain WP3-S18-CRE-02
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP022010 (8351..8488 [-], 138 nt)
oriT length   138 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 138 nt

>oriT_pWP3-S18-CRE-02_5
CGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11199 GenBank   WP_059385972
Name   traI_H7R71_RS29065_pWP3-S18-CRE-02_5 insolico UniProt ID   A0A7I8I4L4
Length   890 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 890 a.a.        Molecular weight: 101396.95 Da        Isoelectric Point: 9.8812

>WP_059385972.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Gammaproteobacteria]
MIVKKVKNPQKAASKAVRVSRLTDYIREPERENSQEKCIHAGARGFITDEPQSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINN
GFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAI
EDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQR
QQVAQREPEPIKPDVPGWKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
ASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRV
TSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKGEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(19-273)

Large polyvalent protein-associated domain 7 (LPD7)

(439-527)

RepB DNA-primase from phage plasmid (RepB_primase)

(549-807)


  Protein structure


Source ID Structure
AlphaFold DB A0A7I8I4L4


Host bacterium

ID   18005 GenBank   NZ_AP022010
Plasmid name   pWP3-S18-CRE-02_5 Incompatibility group   IncP6
Plasmid size   31071 bp Coordinate of oriT [Strand]   8351..8488 [-]
Host baterium   Klebsiella quasipneumoniae strain WP3-S18-CRE-02

Cargo genes

Drug resistance gene   aac(6')-Il, blaGES-4, catB3, blaOXA-129, aadA2, qacE, sul1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -