Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   117466
Name   oriT_pELF_mdr in_silico
Organism   Enterococcus faecium strain NUITM-VRE1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP026773 (64110..64147 [-], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      7..12, 16..21  (AGTAAC..GTTACT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT_pELF_mdr
ATACGAAGTAACGAAGTTACTGCGTATAAGTGCGCCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11120 GenBank   WP_309298971
Name   mobQ_OO969_RS13865_pELF_mdr insolico UniProt ID   _
Length   661 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 661 a.a.        Molecular weight: 77279.40 Da        Isoelectric Point: 9.0066

>WP_309298971.1 MobQ family relaxase [Enterococcus faecium]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCRELNVALPIELNNSDQKMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNNWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEEKGVISELKRKNLEIQSYNDILAELDKLESQEKVLKEDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERLFKKSYPSLNIDKFSNQEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQMVKIPNDCQGVFFVQDSMKYID
ELSLLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR

  Protein domains


Predicted by InterproScan.

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(289-394)

MobA/MobL family (MobA_MobL)

(18-242)


  Protein structure



No available structure.




T4CP

ID   12990 GenBank   WP_032495476
Name   t4cp2_OO969_RS13910_pELF_mdr insolico UniProt ID   _
Length   551 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 551 a.a.        Molecular weight: 63052.07 Da        Isoelectric Point: 4.7040

>WP_032495476.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Lactobacillales]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKETIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(33-456)

  Protein structure



No available structure.



ID   12991 GenBank   WP_002353144
Name   tcpA_OO969_RS14110_pELF_mdr insolico UniProt ID   _
Length   458 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 458 a.a.        Molecular weight: 50710.70 Da        Isoelectric Point: 9.2035

>WP_002353144.1 MULTISPECIES: FtsK/SpoIIIE domain-containing protein [Enterococcus]
MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKDPTDAIFGIKPDGTIAHFCISEMPHML
VAGTTGSGKSVLLNEILVTVMCHSTPDELKIGIVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVI
LMHERYKLMEKAGLQNITLYNEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHV
ILATQSPRSTVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQGAYIS
NKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKRNLNTPLANPSSKEPSI
EKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGKADPEPQTSNNTNNKQPIASKTKPKANDP
KGSENVVITTDKSEKKQINGSTSPIMQKMLKAKKQVHK

  Protein domains


Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(59-186)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 66605..78893

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO969_RS13840 (NUITMVRE1_27140) 61684..62001 + 318 WP_002300567 hypothetical protein -
OO969_RS13845 (NUITMVRE1_27150) 62535..63032 + 498 WP_002300565 DnaJ domain-containing protein -
OO969_RS13850 (NUITMVRE1_27160) 63066..63371 + 306 WP_104659454 hypothetical protein -
OO969_RS13855 (NUITMVRE1_27170) 63394..63651 - 258 WP_002325623 hypothetical protein -
OO969_RS13860 (NUITMVRE1_27180) 63654..63953 - 300 WP_002325624 hypothetical protein -
OO969_RS13865 (NUITMVRE1_27190) 64245..66230 + 1986 WP_309298971 MobQ family relaxase -
OO969_RS13870 (NUITMVRE1_27200) 66254..66586 + 333 WP_032495472 CagC family type IV secretion system protein -
OO969_RS13875 (NUITMVRE1_27210) 66605..66988 + 384 WP_001083514 glycosyl transferase trsC
OO969_RS13880 (NUITMVRE1_27220) 67005..67634 + 630 WP_002325627 hypothetical protein trsD
OO969_RS13885 (NUITMVRE1_27230) 67645..69606 + 1962 WP_032495473 VirB4 family type IV secretion system protein virb4
OO969_RS13890 (NUITMVRE1_27240) 69620..70972 + 1353 WP_032495474 hypothetical protein trsF
OO969_RS13895 (NUITMVRE1_27250) 70994..72103 + 1110 WP_000501303 lysozyme family protein orf14
OO969_RS13900 (NUITMVRE1_27260) 72116..72667 + 552 WP_000393725 hypothetical protein -
OO969_RS13905 (NUITMVRE1_27270) 72672..73103 + 432 WP_032495475 hypothetical protein -
OO969_RS13910 (NUITMVRE1_27280) 73096..74751 + 1656 WP_032495476 VirD4-like conjugal transfer protein, CD1115 family -
OO969_RS13915 (NUITMVRE1_27290) 74769..75692 + 924 WP_032495477 hypothetical protein -
OO969_RS13920 (NUITMVRE1_27300) 75692..76636 + 945 WP_194187774 conjugal transfer protein TrbL family protein trsL
OO969_RS13925 (NUITMVRE1_27310) 76653..77624 + 972 WP_002425009 hypothetical protein -
OO969_RS13930 (NUITMVRE1_27320) 77653..78021 + 369 WP_000516429 hypothetical protein -
OO969_RS13935 (NUITMVRE1_27330) 78081..78893 + 813 WP_118368352 LPXTG cell wall anchor domain-containing protein prgC
OO969_RS13940 (NUITMVRE1_27340) 78996..79178 - 183 Protein_89 IS6 family transposase -
OO969_RS13945 (NUITMVRE1_27350) 79232..79912 - 681 WP_013646119 IS6-like element IS1216 family transposase -
OO969_RS13950 (NUITMVRE1_27360) 79989..80771 + 783 Protein_91 RNA-guided endonuclease TnpB family protein -
OO969_RS13955 (NUITMVRE1_27370) 80978..81313 + 336 WP_010729677 hypothetical protein -
OO969_RS13960 (NUITMVRE1_27380) 81570..83153 - 1584 WP_002347719 hypothetical protein -
OO969_RS13965 (NUITMVRE1_27390) 83275..83859 + 585 WP_002347505 hypothetical protein -

Host bacterium

ID   17899 GenBank   NZ_AP026773
Plasmid name   pELF_mdr Incompatibility group   -
Plasmid size   138954 bp Coordinate of oriT [Strand]   64110..64147 [-]
Host baterium   Enterococcus faecium strain NUITM-VRE1

Cargo genes

Drug resistance gene   fexA, poxtA, Cfr(D), erm(B), VanHAX, fosB3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -