Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   117451
Name   oriT_E2|unnamed1 in_silico
Organism   Lactiplantibacillus plantarum strain E2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP110243 (10274..10294 [-], 21 nt)
oriT length   21 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 21 nt

>oriT_E2|unnamed1
AGTGGGGTGTAAGTGCGCATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11115 GenBank   WP_264993783
Name   mobQ_OLJ37_RS15635_E2|unnamed1 insolico UniProt ID   _
Length   687 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 687 a.a.        Molecular weight: 80728.19 Da        Isoelectric Point: 8.6531

>WP_264993783.1 MobQ family relaxase [Lactiplantibacillus plantarum]
MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDKEGRHYFYAQSIMPESFILTPKNSPEWASDREQLWN
EVEKKDRKSNSRYAKEFNVALPVELSESEQKELLTKYVQENFVDEGMVADVAIHRDHADNPHAHVMLTNR
PFNPDGTWGIKSKKQYILDENGNKTYTGTSKYPKSRKILMVDWDKKEKIIEWRHNWAASVNQVLDQKNIP
DRISEKSFVDQGIDEVATQHEGINSKRYERKEFNQQVKNYRNTKASYKNNQEKAINRGHLDSLSKHFSFN
EKRLVNELGHELKTYISLESLDDKRRMLFNWKNSTLIKHAIGEDVTKKLLTINQQESSLKKADELLNKVV
DRTTKKLYPELDFEQTTQAERRELIKETESEQTIFKGSELNERLMNICDDLLTRQLLVFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSSTEQQKITRAVKDLRTIMAVEQVIQTQYQEVLR
RTFPNGNFNELPMIKQEQAYTAVMYYDPALKPCKVETIAQWQENPPRVFTTQEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFLGECKADPMIKNSQIEKIQKQLKEQQAKDDQYRKANMGHYQPLNYKPVSPDY
YLKTAFSDAIMTVLYARDEDYQRQKQERGLKETEWEMTKKQRQHQTRNRHEDGGRHL

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-241)

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(276-384)


  Protein structure



No available structure.




T4CP

ID   12986 GenBank   WP_264993792
Name   t4cp2_OLJ37_RS15695_E2|unnamed1 insolico UniProt ID   _
Length   516 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 516 a.a.        Molecular weight: 57999.14 Da        Isoelectric Point: 4.8844

>WP_264993792.1 VirD4-like conjugal transfer protein, CD1115 family [Lactiplantibacillus plantarum]
MNGTILGIVDKKIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKTAGVK
LAQGYQVHVVNFANMAHSDRYNPFDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMK
HRSPEQRNLAGVTQVLQENDVEAEEKGADSPLDTLFLDLTMSDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDQEVADFTSFSDFDLKEIGNAKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKDKAESILGNHAVKICLNSANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPENQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNEYQGQPEASQETNFHQKVAKWEAKLKETAQKEAASEMSQKEEEQQQDNL
DSDLERVSNKDSQTEEPEEDDKNLIG

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(24-443)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 13862..23951

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OLJ37_RS15615 (OLJ37_15615) 8997..9187 + 191 Protein_7 hypothetical protein -
OLJ37_RS15620 (OLJ37_15620) 9202..9627 + 426 WP_264993782 zeta toxin family protein -
OLJ37_RS15625 (OLJ37_15625) 9617..9895 - 279 WP_003645706 hypothetical protein -
OLJ37_RS15630 (OLJ37_15630) 9912..10124 - 213 WP_003645707 hypothetical protein -
OLJ37_RS15635 (OLJ37_15635) 10396..12459 + 2064 WP_264993783 MobQ family relaxase -
OLJ37_RS15640 (OLJ37_15640) 12543..12854 + 312 WP_264993784 hypothetical protein -
OLJ37_RS15645 (OLJ37_15645) 12890..13504 + 615 WP_264993785 hypothetical protein -
OLJ37_RS15650 (OLJ37_15650) 13506..13841 + 336 WP_024855764 CagC family type IV secretion system protein -
OLJ37_RS15655 (OLJ37_15655) 13862..14224 + 363 WP_195480458 conjugal transfer protein trsC
OLJ37_RS15660 (OLJ37_15660) 14193..14852 + 660 WP_068806586 TrsD/TraD family conjugative transfer protein trsD
OLJ37_RS15665 (OLJ37_15665) 14864..16882 + 2019 WP_264993786 VirB4 family type IV secretion system protein virb4
OLJ37_RS15670 (OLJ37_15670) 16875..18293 + 1419 WP_264993787 conjugal transfer protein trsF
OLJ37_RS15675 (OLJ37_15675) 18294..19451 + 1158 WP_264993788 phage tail tip lysozyme prgK
OLJ37_RS15680 (OLJ37_15680) 19466..20083 + 618 WP_264993789 hypothetical protein -
OLJ37_RS15685 (OLJ37_15685) 20070..20438 + 369 WP_264993790 thioredoxin family protein -
OLJ37_RS15690 (OLJ37_15690) 20439..20909 + 471 WP_264993791 conjugal transfer protein trsJ
OLJ37_RS15695 (OLJ37_15695) 21139..22689 + 1551 WP_264993792 VirD4-like conjugal transfer protein, CD1115 family -
OLJ37_RS15700 (OLJ37_15700) 22704..23093 + 390 WP_264993793 hypothetical protein -
OLJ37_RS15705 (OLJ37_15705) 23112..23951 + 840 WP_060599186 conjugal transfer protein TrbL family protein trsL
OLJ37_RS15710 (OLJ37_15710) 23966..24376 + 411 WP_264993794 hypothetical protein -
OLJ37_RS15715 (OLJ37_15715) 24383..26530 + 2148 WP_264993795 type IA DNA topoisomerase -
OLJ37_RS15720 (OLJ37_15720) 26687..28009 - 1323 WP_264993796 DNA (cytosine-5-)-methyltransferase -
OLJ37_RS15725 (OLJ37_15725) 28274..28687 + 414 Protein_29 IS30 family transposase -

Host bacterium

ID   17884 GenBank   NZ_CP110243
Plasmid name   E2|unnamed1 Incompatibility group   -
Plasmid size   54805 bp Coordinate of oriT [Strand]   10274..10294 [-]
Host baterium   Lactiplantibacillus plantarum strain E2

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -