Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 117402 |
| Name | oriT_p13450-2  |
| Organism | Klebsiella variicola strain 13450 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP026016 (11576..11625 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_p13450-2
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 12949 | GenBank | WP_060598860 |
| Name | traD_C2D62_RS28840_p13450-2 |
UniProt ID | _ |
| Length | 766 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 766 a.a. Molecular weight: 85363.18 Da Isoelectric Point: 4.9167
>WP_060598860.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPDDAGSHAGEQPELASQPA
PAEVTVSPAPVKAPATTTMPAAEPSPRTAEPPVLRVTTVPLIKPKAAAAASTASSAGNPAAAAGGTEQEL
AQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPDDAGSHAGEQPELASQPA
PAEVTVSPAPVKAPATTTMPAAEPSPRTAEPPVLRVTTVPLIKPKAAAAASTASSAGNPAAAAGGTEQEL
AQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 14083..41724
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C2D62_RS28645 (C2D62_29860) | 10456..10986 | + | 531 | WP_077138878 | antirestriction protein | - |
| C2D62_RS28650 (C2D62_29865) | 11024..11431 | - | 408 | WP_224230374 | transglycosylase SLT domain-containing protein | - |
| C2D62_RS28655 (C2D62_29870) | 11925..12323 | + | 399 | WP_060598876 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| C2D62_RS28660 (C2D62_29875) | 12496..13182 | + | 687 | WP_060598875 | transcriptional regulator TraJ family protein | - |
| C2D62_RS28665 (C2D62_29880) | 13261..13647 | + | 387 | WP_004152495 | TraY domain-containing protein | - |
| C2D62_RS28670 (C2D62_29885) | 13701..14069 | + | 369 | WP_049155512 | type IV conjugative transfer system pilin TraA | - |
| C2D62_RS28675 (C2D62_29890) | 14083..14388 | + | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| C2D62_RS28680 (C2D62_29895) | 14408..14974 | + | 567 | WP_153242763 | type IV conjugative transfer system protein TraE | traE |
| C2D62_RS28685 (C2D62_29900) | 14961..15701 | + | 741 | WP_023340945 | type-F conjugative transfer system secretin TraK | traK |
| C2D62_RS28690 (C2D62_29905) | 15701..17125 | + | 1425 | WP_153242766 | F-type conjugal transfer pilus assembly protein TraB | traB |
| C2D62_RS28695 (C2D62_29910) | 17118..17714 | + | 597 | WP_060598872 | conjugal transfer pilus-stabilizing protein TraP | - |
| C2D62_RS28700 (C2D62_29915) | 17737..18321 | + | 585 | WP_060598871 | type IV conjugative transfer system lipoprotein TraV | traV |
| C2D62_RS28705 (C2D62_29920) | 18453..18863 | + | 411 | WP_060598870 | hypothetical protein | - |
| C2D62_RS28710 (C2D62_29925) | 18868..19152 | + | 285 | WP_153242804 | hypothetical protein | - |
| C2D62_RS28715 (C2D62_29930) | 19176..19400 | + | 225 | WP_060598869 | hypothetical protein | - |
| C2D62_RS28720 (C2D62_29935) | 19393..19704 | + | 312 | WP_077138882 | hypothetical protein | - |
| C2D62_RS28725 (C2D62_29940) | 19771..20175 | + | 405 | WP_060598867 | hypothetical protein | - |
| C2D62_RS28730 (C2D62_29945) | 20218..20541 | + | 324 | WP_072124277 | hypothetical protein | - |
| C2D62_RS28735 (C2D62_29950) | 20549..20947 | + | 399 | WP_164876867 | hypothetical protein | - |
| C2D62_RS28740 (C2D62_29955) | 21019..23658 | + | 2640 | WP_153242768 | type IV secretion system protein TraC | virb4 |
| C2D62_RS28745 (C2D62_29960) | 23658..24047 | + | 390 | WP_020803372 | type-F conjugative transfer system protein TrbI | - |
| C2D62_RS28750 (C2D62_29965) | 24047..24673 | + | 627 | WP_020314628 | type-F conjugative transfer system protein TraW | traW |
| C2D62_RS28755 (C2D62_29970) | 24717..25676 | + | 960 | WP_029497356 | conjugal transfer pilus assembly protein TraU | traU |
| C2D62_RS28760 (C2D62_29975) | 25691..26239 | + | 549 | WP_022631518 | hypothetical protein | - |
| C2D62_RS28765 (C2D62_29980) | 26214..26903 | - | 690 | WP_032427592 | hypothetical protein | - |
| C2D62_RS28770 (C2D62_29985) | 26960..27562 | + | 603 | WP_077138883 | hypothetical protein | - |
| C2D62_RS28775 (C2D62_29990) | 27707..28354 | + | 648 | WP_039698503 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| C2D62_RS28780 (C2D62_29995) | 28413..30368 | + | 1956 | WP_153242770 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| C2D62_RS28785 (C2D62_30000) | 30401..30682 | + | 282 | WP_022644736 | hypothetical protein | - |
| C2D62_RS28790 (C2D62_30005) | 30672..30899 | + | 228 | WP_153242772 | conjugal transfer protein TrbE | - |
| C2D62_RS28795 (C2D62_30010) | 30910..31236 | + | 327 | WP_012539967 | hypothetical protein | - |
| C2D62_RS28800 (C2D62_30015) | 31257..32009 | + | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| C2D62_RS28805 (C2D62_30020) | 32020..32259 | + | 240 | WP_023340931 | type-F conjugative transfer system pilin chaperone TraQ | - |
| C2D62_RS28810 (C2D62_30025) | 32231..32788 | + | 558 | WP_032433944 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| C2D62_RS28815 (C2D62_30030) | 32834..33277 | + | 444 | WP_040172400 | F-type conjugal transfer protein TrbF | - |
| C2D62_RS28820 (C2D62_30035) | 33255..34634 | + | 1380 | WP_072198238 | conjugal transfer pilus assembly protein TraH | traH |
| C2D62_RS28825 (C2D62_30040) | 34634..37456 | + | 2823 | WP_153242774 | conjugal transfer mating-pair stabilization protein TraG | traG |
| C2D62_RS28830 | 37480..38082 | + | 603 | WP_048263624 | hypothetical protein | - |
| C2D62_RS28835 (C2D62_30045) | 38333..39064 | + | 732 | WP_016831056 | conjugal transfer complement resistance protein TraT | - |
| C2D62_RS28840 (C2D62_30050) | 39424..41724 | + | 2301 | WP_060598860 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 17835 | GenBank | NZ_CP026016 |
| Plasmid name | p13450-2 | Incompatibility group | IncFII |
| Plasmid size | 109205 bp | Coordinate of oriT [Strand] | 11576..11625 [-] |
| Host baterium | Klebsiella variicola strain 13450 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |