Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   117399
Name   oriT_pTH114-3 in_silico
Organism   Klebsiella quasipneumoniae strain TH114
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP035208 (16646..16727 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_pTH114-3
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11087 GenBank   WP_032440569
Name   traI_EQH50_RS25125_pTH114-3 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   12948 GenBank   WP_196611376
Name   trbC_EQH50_RS25260_pTH114-3 insolico UniProt ID   _
Length   710 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 710 a.a.        Molecular weight: 80321.08 Da        Isoelectric Point: 5.7665

>WP_196611376.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Klebsiella]
MVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFRMPARMPMDMDRLDPSTETMR
PGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLILMATTGAGKTEMLLGFVLNA
LCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQRLLENDKSRQQSNNTNPFALA
QETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQRLIQEYLSLDKFAELYLQAE
AEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYNRMLSLFSDTYGHVFSSGAGD
IDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERMEGKAEDILVIKKFRDRFPFL
WLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLLGNMFTRIFGTIIDPTETAEF
AAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALNQGEQATVFKGAVIRNNALYI
ADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMAIVKNDKIRHAPDSMIIQDGV
LYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEPEKLSVKKDELEKYQHIHQSA
VTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(419-540)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 25836..47751

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH50_RS25135 (EQH50_25440) 20983..21477 - 495 WP_032440571 molybdopterin-dependent oxidoreductase -
EQH50_RS25140 (EQH50_25445) 21470..22399 - 930 WP_032440581 glycosyltransferase family 9 protein -
EQH50_RS25145 (EQH50_25450) 22459..23301 - 843 WP_032440572 helix-turn-helix domain-containing protein -
EQH50_RS25150 (EQH50_25455) 23911..24138 + 228 WP_032440573 hypothetical protein -
EQH50_RS25155 (EQH50_25460) 24870..25349 + 480 WP_032440574 lytic transglycosylase domain-containing protein -
EQH50_RS25160 (EQH50_25465) 25375..25839 + 465 WP_225546581 DotD/TraH family lipoprotein -
EQH50_RS25165 (EQH50_25470) 25836..26654 + 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
EQH50_RS25170 (EQH50_25475) 26632..27819 + 1188 WP_077255878 plasmid transfer ATPase TraJ virB11
EQH50_RS25175 (EQH50_25480) 27816..28082 + 267 WP_032440577 IcmT/TraK family protein traK
EQH50_RS26075 28100..31495 + 3396 WP_162992597 LPD7 domain-containing protein -
EQH50_RS25185 (EQH50_25490) 31529..31882 + 354 WP_032440584 hypothetical protein traL
EQH50_RS25190 (EQH50_25495) 31954..32622 + 669 WP_032440585 DotI/IcmL/TraM family protein traM
EQH50_RS25195 (EQH50_25500) 32633..33736 + 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
EQH50_RS25200 (EQH50_25505) 33740..35080 + 1341 WP_032440586 conjugal transfer protein TraO traO
EQH50_RS25205 (EQH50_25510) 35087..35806 + 720 WP_050597333 conjugal transfer protein TraP traP
EQH50_RS25210 (EQH50_25515) 35816..36349 + 534 WP_032440587 conjugal transfer protein TraQ traQ
EQH50_RS25215 (EQH50_25520) 36405..36806 + 402 WP_032440588 DUF6750 family protein traR
EQH50_RS25220 (EQH50_25525) 36850..37413 + 564 WP_045325070 hypothetical protein traT
EQH50_RS25225 (EQH50_25530) 37423..40470 + 3048 WP_152651986 ATP-binding protein traU
EQH50_RS25230 (EQH50_25535) 40480..41676 + 1197 WP_032440590 conjugal transfer protein TraW traW
EQH50_RS25235 (EQH50_25540) 41673..42254 + 582 WP_050597335 conjugal transfer protein TraX -
EQH50_RS25240 (EQH50_25545) 42296..44440 + 2145 WP_032440591 DotA/TraY family protein traY
EQH50_RS25245 (EQH50_25550) 44506..45129 + 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
EQH50_RS25250 (EQH50_25555) 45231..46649 + 1419 WP_063666705 hypothetical protein trbA
EQH50_RS25255 (EQH50_25560) 46630..47751 + 1122 WP_052455213 thioredoxin fold domain-containing protein trbB
EQH50_RS25260 (EQH50_25565) 47813..49945 + 2133 WP_196611376 F-type conjugative transfer protein TrbC -
EQH50_RS25265 (EQH50_25570) 49991..50524 + 534 WP_032440594 phospholipase D family protein -
EQH50_RS25270 (EQH50_25575) 50536..50787 + 252 WP_113394424 hypothetical protein -
EQH50_RS25275 (EQH50_25580) 51362..51988 + 627 WP_254913868 RepA family replication protein -

Host bacterium

ID   17832 GenBank   NZ_CP035208
Plasmid name   pTH114-3 Incompatibility group   IncFII
Plasmid size   59814 bp Coordinate of oriT [Strand]   16646..16727 [+]
Host baterium   Klebsiella quasipneumoniae strain TH114

Cargo genes

Drug resistance gene   mcr-3.21
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -