Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 117380 |
| Name | oriT_AJ055|2  |
| Organism | Klebsiella variicola strain AJ055 isolate AJ055 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LR130540 (141053..141102 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_AJ055|2
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11077 | GenBank | WP_117122748 |
| Name | traI_EW050_RS27690_AJ055|2 |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190876.90 Da Isoelectric Point: 6.1909
>WP_117122748.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKTLFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQTLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITSLTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLTTALETGGDKIPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEQAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGKARKAGEP
VERVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKTLFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQTLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITSLTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLTTALETGGDKIPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEQAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGKARKAGEP
VERVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12938 | GenBank | WP_111460473 |
| Name | traD_EW050_RS27695_AJ055|2 |
UniProt ID | _ |
| Length | 768 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 768 a.a. Molecular weight: 85876.99 Da Isoelectric Point: 5.0689
>WP_111460473.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIAIIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSQPA
PAEVTVSPAPVKAPPTTKSPAAEPSARAAEPPVLRVTTVPLIKPKAAAAAAAAATASSVGTPAAGGTEQE
LAQQSAEQGQEMLPTGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINRSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIAIIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPVSQPA
PAEVTVSPAPVKAPPTTKSPAAEPSARAAEPPVLRVTTVPLIKPKAAAAAAAAATASSVGTPAAGGTEQE
LAQQSAEQGQEMLPTGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINRSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 112611..138555
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EW050_RS27695 | 112611..114917 | - | 2307 | WP_111460473 | type IV conjugative transfer system coupling protein TraD | virb4 |
| EW050_RS28820 | 114991..115086 | - | 96 | Protein_126 | hypothetical protein | - |
| EW050_RS27700 | 115279..116010 | - | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| EW050_RS27705 | 116200..116727 | - | 528 | WP_047665858 | conjugal transfer protein TraS | - |
| EW050_RS27710 | 116733..119582 | - | 2850 | WP_117122749 | conjugal transfer mating-pair stabilization protein TraG | traG |
| EW050_RS27715 | 119582..120952 | - | 1371 | WP_047665852 | conjugal transfer pilus assembly protein TraH | traH |
| EW050_RS27720 | 120939..121361 | - | 423 | WP_111460475 | hypothetical protein | - |
| EW050_RS27725 | 121354..121926 | - | 573 | WP_055323574 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| EW050_RS27730 | 121898..122137 | - | 240 | WP_009309875 | type-F conjugative transfer system pilin chaperone TraQ | - |
| EW050_RS27735 | 122148..122900 | - | 753 | WP_047665849 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| EW050_RS27740 | 122921..123247 | - | 327 | WP_111460476 | hypothetical protein | - |
| EW050_RS27745 | 123258..123485 | - | 228 | WP_047665846 | conjugal transfer protein TrbE | - |
| EW050_RS27750 | 123547..123756 | - | 210 | WP_111460477 | hypothetical protein | - |
| EW050_RS27755 | 123789..125744 | - | 1956 | WP_111460478 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| EW050_RS27760 | 125803..126441 | - | 639 | WP_047667531 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| EW050_RS27765 | 126454..127443 | - | 990 | WP_111460479 | conjugal transfer pilus assembly protein TraU | traU |
| EW050_RS27770 | 127440..127829 | - | 390 | WP_032445235 | hypothetical protein | - |
| EW050_RS27775 | 127871..128497 | - | 627 | WP_004152507 | type-F conjugative transfer system protein TraW | traW |
| EW050_RS27780 | 128497..128886 | - | 390 | WP_020803372 | type-F conjugative transfer system protein TrbI | - |
| EW050_RS27785 | 128886..131525 | - | 2640 | WP_129671157 | type IV secretion system protein TraC | virb4 |
| EW050_RS27790 | 131597..131995 | - | 399 | WP_074188112 | hypothetical protein | - |
| EW050_RS27795 | 132003..132392 | - | 390 | WP_129671159 | hypothetical protein | - |
| EW050_RS28825 | 132435..132838 | - | 404 | Protein_147 | hypothetical protein | - |
| EW050_RS27805 | 132905..133216 | - | 312 | WP_032740692 | hypothetical protein | - |
| EW050_RS27810 | 133217..133435 | - | 219 | WP_032740686 | hypothetical protein | - |
| EW050_RS27815 | 133459..133749 | - | 291 | WP_032740684 | hypothetical protein | - |
| EW050_RS28830 | 133778..134176 | - | 399 | WP_223170083 | hypothetical protein | - |
| EW050_RS27825 | 134332..134901 | - | 570 | WP_046622829 | type IV conjugative transfer system lipoprotein TraV | traV |
| EW050_RS27830 | 134924..135520 | - | 597 | WP_111460481 | conjugal transfer pilus-stabilizing protein TraP | - |
| EW050_RS27835 | 135513..136937 | - | 1425 | WP_046622826 | F-type conjugal transfer pilus assembly protein TraB | traB |
| EW050_RS27840 | 136937..137677 | - | 741 | WP_013214019 | type-F conjugative transfer system secretin TraK | traK |
| EW050_RS27845 | 137664..138230 | - | 567 | WP_047665763 | type IV conjugative transfer system protein TraE | traE |
| EW050_RS27850 | 138250..138555 | - | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| EW050_RS27855 | 138569..138937 | - | 369 | WP_047665762 | type IV conjugative transfer system pilin TraA | - |
| EW050_RS27860 | 138991..139362 | - | 372 | WP_004208838 | TraY domain-containing protein | - |
| EW050_RS27865 | 139446..140141 | - | 696 | WP_111460482 | transcriptional regulator TraJ family protein | - |
| EW050_RS27870 | 140348..140740 | - | 393 | WP_049155510 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| EW050_RS27875 | 141247..141654 | + | 408 | WP_023292159 | transglycosylase SLT domain-containing protein | - |
| EW050_RS27880 | 141692..142222 | - | 531 | WP_110183759 | antirestriction protein | - |
Host bacterium
| ID | 17813 | GenBank | NZ_LR130540 |
| Plasmid name | AJ055|2 | Incompatibility group | IncFIB |
| Plasmid size | 165705 bp | Coordinate of oriT [Strand] | 141053..141102 [+] |
| Host baterium | Klebsiella variicola strain AJ055 isolate AJ055 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsH, arsC, arsB, arsA, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |