Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 117146 |
| Name | oriT_pBE15_3  |
| Organism | Enterococcus faecalis strain BE15 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP110066 (20561..20598 [+], 38 nt) |
| oriT length | 38 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 38 nt
>oriT_pBE15_3
ATACGAAGTAATGAAGTTACTGCGTATAAGTGCGCCCT
ATACGAAGTAATGAAGTTACTGCGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10919 | GenBank | WP_319800784 |
| Name | mobQ_OLL96_RS14780_pBE15_3 |
UniProt ID | _ |
| Length | 661 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 661 a.a. Molecular weight: 77253.36 Da Isoelectric Point: 9.4365
>WP_319800784.1 MobQ family relaxase [Enterococcus faecalis]
MAIFHLSMTIAKRENGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNEWGRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQEKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIEKTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR
MAIFHLSMTIAKRENGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNEWGRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQEKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIEKTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12751 | GenBank | WP_010723050 |
| Name | t4cp2_OLL96_RS14730_pBE15_3 |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63057.09 Da Isoelectric Point: 4.7399
>WP_010723050.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MSSLLAESDGLILGKFPSGKVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELSMSDILSSFDEGIEENTANNDNSELPF
MSSLLAESDGLILGKFPSGKVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELSMSDILSSFDEGIEENTANNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 5951..18103
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OLL96_RS14670 (OLL96_14670) | 2241..2540 | - | 300 | WP_047342400 | hypothetical protein | - |
| OLL96_RS14675 (OLL96_14675) | 2906..3178 | - | 273 | WP_011266100 | helix-turn-helix transcriptional regulator | - |
| OLL96_RS14680 (OLL96_14680) | 3615..3917 | - | 303 | WP_141427078 | ATP-binding protein | - |
| OLL96_RS14685 (OLL96_14685) | 4070..4465 | - | 396 | WP_141427077 | Lrp/AsnC family transcriptional regulator | - |
| OLL96_RS14690 (OLL96_14690) | 4569..5006 | + | 438 | WP_069854469 | VOC family protein | - |
| OLL96_RS14695 (OLL96_14695) | 5178..5351 | - | 174 | WP_264526519 | hypothetical protein | - |
| OLL96_RS14700 (OLL96_14700) | 5578..5676 | + | 99 | WP_264526520 | type I toxin-antitoxin system Fst family toxin | - |
| OLL96_RS14705 (OLL96_14705) | 5951..6667 | - | 717 | WP_264526521 | LPXTG cell wall anchor domain-containing protein | prgC |
| OLL96_RS14710 (OLL96_14710) | 6727..7095 | - | 369 | WP_000516429 | hypothetical protein | - |
| OLL96_RS14715 (OLL96_14715) | 7124..8095 | - | 972 | WP_002425009 | hypothetical protein | - |
| OLL96_RS14720 (OLL96_14720) | 8112..9044 | - | 933 | WP_010750197 | conjugal transfer protein TrbL family protein | trsL |
| OLL96_RS14725 (OLL96_14725) | 9044..9967 | - | 924 | WP_264526522 | hypothetical protein | traP |
| OLL96_RS14730 (OLL96_14730) | 9985..11640 | - | 1656 | WP_010723050 | VirD4-like conjugal transfer protein, CD1115 family | - |
| OLL96_RS14735 (OLL96_14735) | 11633..12064 | - | 432 | WP_010723049 | hypothetical protein | - |
| OLL96_RS14740 (OLL96_14740) | 12069..12620 | - | 552 | WP_010723048 | hypothetical protein | - |
| OLL96_RS14745 (OLL96_14745) | 12633..13742 | - | 1110 | WP_264526523 | lysozyme family protein | orf14 |
| OLL96_RS14750 (OLL96_14750) | 13764..14588 | - | 825 | WP_264560707 | hypothetical protein | - |
| OLL96_RS14755 (OLL96_14755) | 14576..15088 | - | 513 | WP_264560704 | hypothetical protein | trsF |
| OLL96_RS14760 (OLL96_14760) | 15102..17063 | - | 1962 | WP_264526525 | VirB4 family type IV secretion system protein | virb4 |
| OLL96_RS14765 (OLL96_14765) | 17074..17703 | - | 630 | WP_319800785 | hypothetical protein | trsD |
| OLL96_RS14770 (OLL96_14770) | 17720..18103 | - | 384 | WP_002332651 | hypothetical protein | trsC |
| OLL96_RS14775 (OLL96_14775) | 18122..18454 | - | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| OLL96_RS14780 (OLL96_14780) | 18478..20463 | - | 1986 | WP_319800784 | MobQ family relaxase | - |
| OLL96_RS14785 (OLL96_14785) | 20755..21054 | + | 300 | WP_264560705 | hypothetical protein | - |
| OLL96_RS14790 (OLL96_14790) | 21057..21329 | + | 273 | WP_264560706 | hypothetical protein | - |
| OLL96_RS14795 (OLL96_14795) | 21336..21641 | - | 306 | WP_264526512 | hypothetical protein | - |
| OLL96_RS14800 (OLL96_14800) | 21675..22172 | - | 498 | WP_002300565 | DnaJ domain-containing protein | - |
| OLL96_RS14805 (OLL96_14805) | 22192..22509 | - | 318 | WP_002338433 | hypothetical protein | - |
Host bacterium
| ID | 17579 | GenBank | NZ_CP110066 |
| Plasmid name | pBE15_3 | Incompatibility group | - |
| Plasmid size | 28923 bp | Coordinate of oriT [Strand] | 20561..20598 [+] |
| Host baterium | Enterococcus faecalis strain BE15 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |