Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116998 |
| Name | oriT_pT17-1-optrA-57k  |
| Organism | Enterococcus faecium strain T17-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP109840 (54012..54041 [+], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT_pT17-1-optrA-57k
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10844 | GenBank | WP_233701194 |
| Name | mobQ_OKL57_RS13090_pT17-1-optrA-57k |
UniProt ID | _ |
| Length | 661 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 661 a.a. Molecular weight: 77313.36 Da Isoelectric Point: 9.2532
>WP_233701194.1 MULTISPECIES: MobQ family relaxase [Lactobacillales]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKYID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKYID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12659 | GenBank | WP_002301639 |
| Name | t4cp2_OKL57_RS13045_pT17-1-optrA-57k |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63116.11 Da Isoelectric Point: 4.7018
>WP_002301639.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIDENTTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIDENTTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 31597..51546
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OKL57_RS12965 (OKL57_12960) | 26752..27348 | - | 597 | WP_005237668 | PBECR4 domain-containing protein | - |
| OKL57_RS12970 (OKL57_12965) | 27565..28689 | - | 1125 | WP_235692367 | AAA family ATPase | - |
| OKL57_RS12975 (OKL57_12970) | 28691..29416 | - | 726 | WP_016630100 | DUF6414 family protein | - |
| OKL57_RS12980 (OKL57_12975) | 29429..29893 | - | 465 | WP_235692368 | toll/interleukin-1 receptor domain-containing protein | - |
| OKL57_RS12985 (OKL57_12980) | 29979..30659 | - | 681 | WP_000191454 | IS6-like element ISEnfa1 family transposase | - |
| OKL57_RS12990 (OKL57_12985) | 30715..31494 | - | 780 | Protein_41 | IS256 family transposase | - |
| OKL57_RS12995 (OKL57_12990) | 31597..32463 | - | 867 | WP_263751897 | LPXTG cell wall anchor domain-containing protein | prgC |
| OKL57_RS13000 (OKL57_12995) | 32876..35974 | - | 3099 | WP_002301650 | HsdR family type I site-specific deoxyribonuclease | - |
| OKL57_RS13005 (OKL57_13000) | 35977..37182 | - | 1206 | WP_002325634 | restriction endonuclease subunit S | - |
| OKL57_RS13010 (OKL57_13005) | 37179..38726 | - | 1548 | WP_002301648 | type I restriction-modification system subunit M | - |
| OKL57_RS13015 (OKL57_13010) | 39219..39464 | - | 246 | WP_002301647 | hypothetical protein | - |
| OKL57_RS13020 (OKL57_13015) | 39711..40088 | + | 378 | WP_002325633 | hypothetical protein | - |
| OKL57_RS13025 (OKL57_13020) | 40148..40501 | - | 354 | WP_002301645 | hypothetical protein | - |
| OKL57_RS13030 (OKL57_13025) | 40530..41498 | - | 969 | WP_002301644 | hypothetical protein | - |
| OKL57_RS13035 (OKL57_13030) | 41515..42459 | - | 945 | WP_002301643 | conjugal transfer protein TrbL family protein | trsL |
| OKL57_RS13040 (OKL57_13035) | 42459..43382 | - | 924 | WP_002301641 | hypothetical protein | - |
| OKL57_RS13045 (OKL57_13040) | 43400..45055 | - | 1656 | WP_002301639 | VirD4-like conjugal transfer protein, CD1115 family | - |
| OKL57_RS13050 (OKL57_13045) | 45048..45479 | - | 432 | WP_002301637 | hypothetical protein | - |
| OKL57_RS13055 (OKL57_13050) | 45484..46035 | - | 552 | WP_021109241 | hypothetical protein | - |
| OKL57_RS13060 (OKL57_13055) | 46048..47157 | - | 1110 | WP_021109240 | lysozyme family protein | orf14 |
| OKL57_RS13065 (OKL57_13060) | 47179..48531 | - | 1353 | WP_104659242 | conjugal transfer protein TraF | trsF |
| OKL57_RS13070 (OKL57_13065) | 48545..50506 | - | 1962 | WP_231428364 | VirB4 family type IV secretion system protein | virb4 |
| OKL57_RS13075 (OKL57_13070) | 50517..51146 | - | 630 | WP_104888277 | hypothetical protein | trsD |
| OKL57_RS13080 (OKL57_13075) | 51163..51546 | - | 384 | WP_001083514 | glycosyl transferase | trsC |
| OKL57_RS13085 (OKL57_13080) | 51565..51897 | - | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| OKL57_RS13090 (OKL57_13085) | 51921..53906 | - | 1986 | WP_233701194 | MobQ family relaxase | - |
| OKL57_RS13095 (OKL57_13090) | 54198..54497 | + | 300 | WP_002325624 | hypothetical protein | - |
| OKL57_RS13100 (OKL57_13095) | 54500..54757 | + | 258 | WP_010717195 | hypothetical protein | - |
| OKL57_RS13105 (OKL57_13100) | 54787..54963 | - | 177 | WP_181728217 | hypothetical protein | - |
| OKL57_RS13110 (OKL57_13105) | 55035..55715 | - | 681 | WP_001015311 | IS6-like element IS1216 family transposase | - |
Host bacterium
| ID | 17431 | GenBank | NZ_CP109840 |
| Plasmid name | pT17-1-optrA-57k | Incompatibility group | - |
| Plasmid size | 57547 bp | Coordinate of oriT [Strand] | 54012..54041 [+] |
| Host baterium | Enterococcus faecium strain T17-1 |
Cargo genes
| Drug resistance gene | optrA, fexA, erm(B), erm(A) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |