Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116970 |
| Name | oriT_ES-108-1|unnamed2  |
| Organism | Enterococcus faecalis strain ES-108-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP138644 (22207..22256 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 34..35 |
| Conserved sequence flanking the nic site |
GCTTGCAAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_ES-108-1|unnamed2
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10832 | GenBank | WP_002393736 |
| Name | Relaxase_SHT68_RS16065_ES-108-1|unnamed2 |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65367.01 Da Isoelectric Point: 6.1207
>WP_002393736.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSKLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSKLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 6370 | GenBank | WP_002393737 |
| Name | WP_002393737_ES-108-1|unnamed2 |
UniProt ID | A0A8E7CBI6 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 13965.01 Da Isoelectric Point: 9.6545
>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12648 | GenBank | WP_319451440 |
| Name | t4cp2_SHT68_RS16085_ES-108-1|unnamed2 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70161.20 Da Isoelectric Point: 6.1811
>WP_319451440.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNMLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNMLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 24900..46442
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SHT68_RS16065 (SHT68_16060) | 20098..21783 | - | 1686 | WP_002393736 | relaxase/mobilization nuclease domain-containing protein | - |
| SHT68_RS16070 (SHT68_16065) | 21784..22140 | - | 357 | WP_002393737 | plasmid mobilization relaxosome protein MobC | - |
| SHT68_RS16075 (SHT68_16070) | 22386..22658 | - | 273 | WP_002362409 | LtrD | - |
| SHT68_RS16080 (SHT68_16075) | 22691..24850 | - | 2160 | WP_319451439 | ArdC-like ssDNA-binding domain-containing protein | - |
| SHT68_RS16085 (SHT68_16080) | 24900..26729 | - | 1830 | WP_319451440 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| SHT68_RS16090 (SHT68_16085) | 26729..27214 | - | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| SHT68_RS16095 (SHT68_16090) | 27256..27438 | - | 183 | WP_002367689 | hypothetical protein | - |
| SHT68_RS16100 (SHT68_16095) | 27484..27942 | - | 459 | WP_002370354 | single-stranded DNA-binding protein | - |
| SHT68_RS16105 (SHT68_16100) | 27959..28714 | - | 756 | WP_002425089 | Fic family protein | - |
| SHT68_RS16110 (SHT68_16105) | 28827..29435 | - | 609 | WP_002367686 | hypothetical protein | - |
| SHT68_RS16115 (SHT68_16110) | 29419..29664 | - | 246 | WP_002393743 | hypothetical protein | - |
| SHT68_RS16120 (SHT68_16115) | 29651..30277 | - | 627 | WP_010831027 | hypothetical protein | prgL |
| SHT68_RS16125 (SHT68_16120) | 30550..32484 | - | 1935 | WP_048948961 | group II intron reverse transcriptase/maturase | - |
| SHT68_RS16130 (SHT68_16125) | 33208..35721 | - | 2514 | WP_319451433 | phage tail tip lysozyme | prgK |
| SHT68_RS16135 (SHT68_16130) | 35733..38123 | - | 2391 | WP_048948777 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| SHT68_RS16140 (SHT68_16135) | 38077..38430 | - | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| SHT68_RS16145 (SHT68_16140) | 38432..39232 | - | 801 | WP_033786932 | type IV secretion system protein | prgHb |
| SHT68_RS16150 (SHT68_16145) | 39247..39543 | - | 297 | WP_010784484 | hypothetical protein | - |
| SHT68_RS16155 (SHT68_16150) | 39556..39783 | - | 228 | WP_002365858 | hypothetical protein | - |
| SHT68_RS16160 (SHT68_16155) | 39830..40264 | - | 435 | WP_002367916 | hypothetical protein | - |
| SHT68_RS16165 (SHT68_16160) | 40284..41183 | - | 900 | WP_002393747 | hypothetical protein | - |
| SHT68_RS16170 (SHT68_16165) | 41225..42082 | - | 858 | WP_002367914 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| SHT68_RS16175 (SHT68_16170) | 42110..42463 | - | 354 | WP_002367913 | pheromone response system RNA-binding regulator PrgU | - |
| SHT68_RS16180 (SHT68_16175) | 42525..46442 | - | 3918 | WP_319451434 | LPXTG-anchored aggregation substance PrgB/Asc10 | prgB |
| SHT68_RS16185 (SHT68_16180) | 46654..46842 | - | 189 | Protein_49 | hypothetical protein | - |
| SHT68_RS16190 (SHT68_16185) | 47115..47309 | - | 195 | WP_002415094 | hypothetical protein | - |
| SHT68_RS16195 (SHT68_16190) | 47388..50060 | - | 2673 | WP_025188189 | surface exclusion protein SEA1/PrgA | - |
Host bacterium
| ID | 17403 | GenBank | NZ_CP138644 |
| Plasmid name | ES-108-1|unnamed2 | Incompatibility group | - |
| Plasmid size | 50088 bp | Coordinate of oriT [Strand] | 22207..22256 [-] |
| Host baterium | Enterococcus faecalis strain ES-108-1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |