Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116954
Name   oriT_p53EVA-2 in_silico
Organism   Enterobacter cloacae strain 53EVA
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP138442 (219216..219500 [+], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_p53EVA-2
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGCCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10825 GenBank   WP_038989148
Name   mobH_SE691_RS24580_p53EVA-2 insolico UniProt ID   A0A7T0P871
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 113008.03 Da        Isoelectric Point: 4.5108

>WP_038989148.1 MULTISPECIES: MobH family relaxase [Enterobacteriaceae]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVLRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYPATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure


Source ID Structure
AlphaFold DB A0A7T0P871


T4CP

ID   12640 GenBank   WP_000167418
Name   traD_SE691_RS24575_p53EVA-2 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 144951..154119

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SE691_RS24140 140406..141659 - 1254 WP_001048869 ParA family protein -
SE691_RS24145 143852..144280 - 429 WP_124061973 hypothetical protein -
SE691_RS24150 144951..147632 - 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4
SE691_RS24155 147641..148591 - 951 WP_001022266 TraV family lipoprotein traV
SE691_RS24160 148601..149353 - 753 WP_010892304 protein-disulfide isomerase HtdT -
SE691_RS24165 149459..149971 - 513 WP_000429933 hypothetical protein -
SE691_RS24170 149980..151338 - 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
SE691_RS24175 151328..151768 - 441 WP_001224093 plasmid transfer protein HtdO -
SE691_RS24180 151770..153002 - 1233 WP_000202110 type-F conjugative transfer system secretin TraK traK
SE691_RS24185 153005..153790 - 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
SE691_RS24190 153802..154119 - 318 WP_000203871 type IV conjugative transfer system protein TraL traL
SE691_RS24195 154171..154524 - 354 WP_038988950 hypothetical protein -
SE691_RS24200 155087..155431 + 345 WP_153253765 hypothetical protein -
SE691_RS24205 155878..156753 - 876 WP_001282731 RepB family plasmid replication initiator protein -
SE691_RS24210 157288..158364 + 1077 WP_001015069 hypothetical protein -
SE691_RS24215 158382..158582 + 201 WP_000357613 hypothetical protein -
SE691_RS24220 158579..158998 + 420 WP_319078179 hypothetical protein -

Host bacterium

ID   17387 GenBank   NZ_CP138442
Plasmid name   p53EVA-2 Incompatibility group   IncFIA
Plasmid size   258558 bp Coordinate of oriT [Strand]   219216..219500 [+]
Host baterium   Enterobacter cloacae strain 53EVA

Cargo genes

Drug resistance gene   tet(M), sul2, tet(X4), floR, dfrA12, aadA2, cmlA1, ant(3'')-Ia, sul3, blaTEM-1B, qnrS1, fosA3
Virulence gene   -
Metal resistance gene   pcoC, pcoB, silA, silB, silF, silC, silR, pcoE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -