Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116947
• Name: oriT_SE2.14|p5
• Organism: Staphylococcus haemolyticus strain SE2.14
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP084240 (638..672 [+], 35 nt)
• oriT length: 35 nt
• IRs (inverted repeats): _
• Location of nic site: 25..26
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 35 nt

>oriT_SE2.14|p5
TTACGAAGTAAATAAGTCTAGTGTGTTATACTTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_RES5_RS11810_SE2.14|p5

• ID: 10818
• GenBank: WP_031766525
• Name: mobV_RES5_RS11810_SE2.14|p5
• UniProt ID: A0A2J6NKK5
• Length: 422 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 422 a.a.
• Molecular weight: 50097.96 Da
• Isoelectric Point: 5.4392

>WP_031766525.1 MULTISPECIES: MobV family relaxase [Bacilli]
MPYSIIRVAKVKSKTNTKGIQKHVQRQNKNYENMDIDLEKSYLNYDLVNDSPIDYNQKIDEKIEQNYNGK
RKIRKDAVKHIDGLITSGNEFFKNQTLEETKQFFEHAKTFLEQEYGKDNLLYATVHMDEKTPHMHYGVVP
ITEDGRLSAKEVLGNKKALTEFQDRFNEHINSCGYDLSRGITRGVTPRRHEQISRYKNLTDYHKEEYEHE
SRKLDRIKQESEEVMEQYQNALDVLKKPINVPYELETEKVGGLFNKETQETGNVVIDKNEFDLLQEQVKA
SQLITDDYEYIKSGKALKDFEEKNKRLEDRLLDEQIKNGKVIDEYNDLADSYNNLLEQNQEKEKELNRSY
KLFNNVFKLIKGVMKEETYHSLINHIDNHLESSKMRETMIVDDNDEQFFKKKYQRHEPEIIFEDERDDGY
TL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-198)

Protein structure

• AlphaFold DB: A0A2J6NKK5

Host bacterium

• ID: 17380
• GenBank: NZ_CP084240
• Plasmid name: SE2.14|p5
• Incompatibility group: -
• Plasmid size: 3685 bp
• Coordinate of oriT [Strand]: 638..672 [+]
• Host baterium: Staphylococcus haemolyticus strain SE2.14

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -