Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116940 |
| Name | oriT_Kluyvera georgiana|1  |
| Organism | Kluyvera georgiana isolate Kluyvera georgiana |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LR882926 (16817..16869 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_Kluyvera georgiana|1
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 12632 | GenBank | WP_015059539 |
| Name | t4cp2_INS41_RS00255_Kluyvera georgiana|1 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 35997..58833
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| INS41_RS00185 | 31219..32343 | - | 1125 | WP_000486716 | site-specific integrase | - |
| INS41_RS00190 | 32392..32700 | + | 309 | WP_106508618 | pilus assembly protein | - |
| INS41_RS00195 | 33243..33398 | - | 156 | WP_001358489 | hypothetical protein | - |
| INS41_RS00410 | 33754..33972 | + | 219 | Protein_41 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| INS41_RS00205 | 33969..35345 | - | 1377 | WP_000750519 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| INS41_RS00210 | 35358..35993 | - | 636 | WP_000934977 | A24 family peptidase | - |
| INS41_RS00215 | 35997..36479 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| INS41_RS00220 | 36545..37108 | - | 564 | WP_034169414 | type 4 pilus major pilin | - |
| INS41_RS00225 | 37158..38267 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| INS41_RS00230 | 38258..39796 | - | 1539 | WP_000466225 | GspE/PulE family protein | virB11 |
| INS41_RS00235 | 39821..40315 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| INS41_RS00240 | 40299..41621 | - | 1323 | WP_000454142 | type 4b pilus protein PilO2 | - |
| INS41_RS00245 | 41660..43303 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| INS41_RS00250 | 43296..43838 | - | 543 | WP_001220544 | sigma 54-interacting transcriptional regulator | virb4 |
| INS41_RS00255 | 43885..45843 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| INS41_RS00260 | 45859..46914 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| INS41_RS00265 | 46933..48072 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| INS41_RS00270 | 48062..48763 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| INS41_RS00275 | 48829..49563 | - | 735 | WP_000432282 | virB8 family protein | virB8 |
| INS41_RS00280 | 49729..52086 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| INS41_RS00285 | 52092..52412 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| INS41_RS00405 | 52483..52773 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| INS41_RS00295 | 52773..53357 | - | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| INS41_RS00300 | 53378..53776 | - | 399 | WP_001153669 | hypothetical protein | - |
| INS41_RS00305 | 53895..54332 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| INS41_RS00310 | 54338..55573 | - | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| INS41_RS00315 | 55576..55875 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| INS41_RS00320 | 55923..56732 | + | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| INS41_RS00325 | 56955..57179 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| INS41_RS00330 | 57188..57832 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| INS41_RS00335 | 57838..58833 | - | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| INS41_RS00340 | 58837..59094 | - | 258 | WP_000739144 | hypothetical protein | - |
| INS41_RS00345 | 59091..59444 | - | 354 | WP_223286767 | hypothetical protein | - |
| INS41_RS00350 | 59664..60110 | - | 447 | WP_001243165 | hypothetical protein | - |
| INS41_RS00355 | 60121..60291 | - | 171 | WP_000550720 | hypothetical protein | - |
| INS41_RS00360 | 60295..60738 | - | 444 | WP_000964330 | NfeD family protein | - |
| INS41_RS00365 | 61112..62065 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| INS41_RS00370 | 62092..62268 | - | 177 | WP_000753050 | hypothetical protein | - |
| INS41_RS00375 | 62261..62476 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| INS41_RS00380 | 62469..62975 | - | 507 | WP_001326595 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 17373 | GenBank | NZ_LR882926 |
| Plasmid name | Kluyvera georgiana|1 | Incompatibility group | IncI2 |
| Plasmid size | 64053 bp | Coordinate of oriT [Strand] | 16817..16869 [-] |
| Host baterium | Kluyvera georgiana isolate Kluyvera georgiana |
Cargo genes
| Drug resistance gene | blaCTX-M-64, mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |