Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116901
Name   oriT_pPROV081-2 in_silico
Organism   Providencia sp. PROV081
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP096344 (37790..37940 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pPROV081-2
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10782 GenBank   WP_088212660
Name   mobH_M1F15_RS21590_pPROV081-2 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109452.09 Da        Isoelectric Point: 4.9964

>WP_088212660.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPRLDANKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQPKEKKVDVTSPVEEPQRK
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   12599 GenBank   WP_000178857
Name   traD_M1F15_RS21595_pPROV081-2 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   12600 GenBank   WP_088212670
Name   traC_M1F15_RS21675_pPROV081-2 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92632.91 Da        Isoelectric Point: 5.9216

>WP_088212670.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYVGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(21-255)

Family of unknown function (DUF5934) (DUF5934)

(283-434)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 35874..61020

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1F15_RS21580 31905..32138 - 234 WP_001191890 hypothetical protein -
M1F15_RS21585 32120..32737 - 618 WP_001249395 hypothetical protein -
M1F15_RS21590 32905..35877 + 2973 WP_088212660 MobH family relaxase -
M1F15_RS21595 35874..37739 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
M1F15_RS21600 37789..38334 + 546 WP_247750980 hypothetical protein -
M1F15_RS21605 38291..38920 + 630 WP_088212662 DUF4400 domain-containing protein tfc7
M1F15_RS21610 38968..39144 + 177 WP_169342285 hypothetical protein -
M1F15_RS21615 39386..39763 + 378 WP_088212663 hypothetical protein -
M1F15_RS21620 39763..40425 + 663 WP_088212664 hypothetical protein -
M1F15_RS21625 40606..40749 + 144 WP_172693090 hypothetical protein -
M1F15_RS21630 40761..41126 + 366 WP_001052531 hypothetical protein -
M1F15_RS21635 41271..41552 + 282 WP_088212665 type IV conjugative transfer system protein TraL traL
M1F15_RS21640 41549..42175 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
M1F15_RS21645 42159..43076 + 918 WP_088212666 type-F conjugative transfer system secretin TraK traK
M1F15_RS21650 43076..44389 + 1314 WP_172693100 TraB/VirB10 family protein traB
M1F15_RS21655 44386..44964 + 579 WP_088212668 type IV conjugative transfer system lipoprotein TraV traV
M1F15_RS21660 44968..45360 + 393 WP_000479535 TraA family conjugative transfer protein -
M1F15_RS21665 45561..51047 + 5487 WP_088212669 Ig-like domain-containing protein -
M1F15_RS21670 51196..51903 + 708 WP_001259346 DsbC family protein -
M1F15_RS21675 51900..54347 + 2448 WP_088212670 type IV secretion system protein TraC virb4
M1F15_RS21680 54362..54679 + 318 WP_000351984 hypothetical protein -
M1F15_RS21685 54676..55206 + 531 WP_001010738 S26 family signal peptidase -
M1F15_RS21690 55169..56434 + 1266 WP_088212671 TrbC family F-type conjugative pilus assembly protein traW
M1F15_RS21695 56431..57102 + 672 WP_088212672 EAL domain-containing protein -
M1F15_RS21700 57102..58106 + 1005 WP_005507569 TraU family protein traU
M1F15_RS21705 58210..61020 + 2811 WP_088212673 conjugal transfer mating pair stabilization protein TraN traN
M1F15_RS21710 61059..61700 - 642 WP_318837464 hypothetical protein -
M1F15_RS21715 62042..62683 - 642 WP_088212675 hypothetical protein -
M1F15_RS21720 62977..63297 + 321 WP_000547566 hypothetical protein -
M1F15_RS21725 63601..63786 + 186 WP_001186917 hypothetical protein -
M1F15_RS21730 64006..64974 + 969 WP_088212676 AAA family ATPase -
M1F15_RS21735 64985..65893 + 909 WP_088212677 hypothetical protein -

Host bacterium

ID   17334 GenBank   NZ_CP096344
Plasmid name   pPROV081-2 Incompatibility group   IncA/C2
Plasmid size   132772 bp Coordinate of oriT [Strand]   37790..37940 [+]
Host baterium   Providencia sp. PROV081

Cargo genes

Drug resistance gene   aph(3')-VIb
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merB, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -