Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116887 |
| Name | oriT_pMUPRSA-2  |
| Organism | Staphylococcus aureus strain N17CSA11 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP107528 (40745..40780 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..9, 15..20 (GCGAAC..GTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pMUPRSA-2
CACGCGAACGGAATGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAATGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10775 | GenBank | WP_001252101 |
| Name | MobA_MobL_OIH35_RS15425_pMUPRSA-2 |
UniProt ID | A0A141HMI9 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>WP_001252101.1 MULTISPECIES: MobA/MobL family protein [Bacilli]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A141HMI9 |
T4CP
| ID | 12589 | GenBank | WP_000209436 |
| Name | t4cp2_OIH35_RS15325_pMUPRSA-2 |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62672.60 Da Isoelectric Point: 7.6569
>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 13163..24384
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OIH35_RS15250 (OIH35_15250) | 8363..8542 | - | 180 | Protein_8 | protein rep | - |
| OIH35_RS15255 (OIH35_15255) | 8819..9142 | + | 324 | WP_031913065 | quaternary ammonium compound efflux SMR transporter QacG | - |
| OIH35_RS15260 (OIH35_15260) | 9754..10599 | - | 846 | Protein_10 | protein rep | - |
| OIH35_RS15265 (OIH35_15265) | 10652..11326 | + | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| OIH35_RS15270 (OIH35_15270) | 11435..11623 | - | 189 | WP_001063224 | hypothetical protein | - |
| OIH35_RS15275 (OIH35_15275) | 11795..12769 | + | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| OIH35_RS15280 (OIH35_15280) | 12786..13103 | + | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| OIH35_RS15285 (OIH35_15285) | 13163..13570 | + | 408 | WP_000110157 | hypothetical protein | trsC |
| OIH35_RS15290 (OIH35_15290) | 13557..14240 | + | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| OIH35_RS15295 (OIH35_15295) | 14261..16273 | + | 2013 | WP_000874919 | VirB4 family type IV secretion system protein | virb4 |
| OIH35_RS15300 (OIH35_15300) | 16285..17565 | + | 1281 | WP_000702361 | membrane protein | trsF |
| OIH35_RS15305 (OIH35_15305) | 17583..18659 | + | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| OIH35_RS15310 (OIH35_15310) | 18669..19154 | + | 486 | WP_000735569 | TrsH/TraH family protein | - |
| OIH35_RS15315 (OIH35_15315) | 19170..21272 | + | 2103 | WP_001094109 | DNA topoisomerase III | - |
| OIH35_RS15320 (OIH35_15320) | 21288..21752 | + | 465 | WP_000715273 | hypothetical protein | trsJ |
| OIH35_RS15325 (OIH35_15325) | 21749..23389 | + | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| OIH35_RS15330 (OIH35_15330) | 23467..24384 | + | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| OIH35_RS15335 (OIH35_15335) | 24401..24793 | + | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| OIH35_RS15340 (OIH35_15340) | 24850..25017 | + | 168 | WP_000869301 | hypothetical protein | - |
| OIH35_RS15345 (OIH35_15345) | 25055..25729 | + | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| OIH35_RS15350 (OIH35_15350) | 25790..26164 | + | 375 | Protein_28 | IS256 family transposase | - |
| OIH35_RS15355 (OIH35_15355) | 26221..26613 | + | 393 | WP_000393259 | GNAT family N-acetyltransferase | - |
| OIH35_RS15360 (OIH35_15360) | 26614..28053 | + | 1440 | WP_001028144 | aminoglycoside O-phosphotransferase APH(2'')-Ia | - |
| OIH35_RS15365 (OIH35_15365) | 28183..28557 | - | 375 | Protein_31 | IS256 family transposase | - |
Host bacterium
| ID | 17320 | GenBank | NZ_CP107528 |
| Plasmid name | pMUPRSA-2 | Incompatibility group | - |
| Plasmid size | 43758 bp | Coordinate of oriT [Strand] | 40745..40780 [+] |
| Host baterium | Staphylococcus aureus strain N17CSA11 |
Cargo genes
| Drug resistance gene | mupA, aac(6')-aph(2'') |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |