Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116809 |
| Name | oriT_FDAARGOS_324|unnamed3  |
| Organism | Enterococcus faecalis strain FDAARGOS_324 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP028283 (45763..45812 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 34..35 |
| Conserved sequence flanking the nic site |
GCTTGCAAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_FDAARGOS_324|unnamed3
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10724 | GenBank | WP_029888123 |
| Name | Relaxase_CEQ02_RS00295_FDAARGOS_324|unnamed3 |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65380.95 Da Isoelectric Point: 6.0136
>WP_029888123.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 6313 | GenBank | WP_002393737 |
| Name | WP_002393737_FDAARGOS_324|unnamed3 |
UniProt ID | A0A8E7CBI6 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 13965.01 Da Isoelectric Point: 9.6545
>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12524 | GenBank | WP_107164624 |
| Name | t4cp2_CEQ02_RS00275_FDAARGOS_324|unnamed3 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70244.27 Da Isoelectric Point: 6.2952
>WP_107164624.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEERKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEERKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 24380..43118
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CEQ02_RS00160 (CEQ02_00160) | 19559..19939 | + | 381 | WP_002369738 | hypothetical protein | - |
| CEQ02_RS00170 (CEQ02_00170) | 20510..20815 | + | 306 | WP_107164620 | hypothetical protein | - |
| CEQ02_RS00175 (CEQ02_00175) | 20826..23498 | + | 2673 | WP_107164621 | surface exclusion protein SEA1/PrgA | - |
| CEQ02_RS00180 (CEQ02_00180) | 23567..24154 | + | 588 | WP_010819640 | hypothetical protein | - |
| CEQ02_RS00185 (CEQ02_00185) | 24380..28297 | + | 3918 | WP_107164622 | LPXTG-anchored aggregation substance PrgB/Asc10 | prgB |
| CEQ02_RS00190 (CEQ02_00190) | 28359..28712 | + | 354 | WP_002367913 | pheromone response system RNA-binding regulator PrgU | - |
| CEQ02_RS00195 (CEQ02_00195) | 28740..29597 | + | 858 | WP_002367673 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| CEQ02_RS00200 (CEQ02_00200) | 29639..30538 | + | 900 | WP_107164623 | type III secretion system protein PrgD | - |
| CEQ02_RS00205 (CEQ02_00205) | 30558..30992 | + | 435 | WP_002365857 | hypothetical protein | - |
| CEQ02_RS00210 (CEQ02_00210) | 31039..31266 | + | 228 | WP_002365858 | hypothetical protein | - |
| CEQ02_RS00215 (CEQ02_00215) | 31279..31575 | + | 297 | WP_010784484 | hypothetical protein | - |
| CEQ02_RS00220 (CEQ02_00220) | 31590..32390 | + | 801 | WP_033786932 | type IV secretion system protein | prgHb |
| CEQ02_RS00225 (CEQ02_00225) | 32392..32745 | + | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| CEQ02_RS00230 (CEQ02_00230) | 32699..35089 | + | 2391 | WP_002365863 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| CEQ02_RS00235 (CEQ02_00235) | 35101..37716 | + | 2616 | WP_002415348 | phage tail tip lysozyme | prgK |
| CEQ02_RS00240 (CEQ02_00240) | 37740..38366 | + | 627 | WP_002395055 | hypothetical protein | prgL |
| CEQ02_RS00245 (CEQ02_00245) | 38353..38598 | + | 246 | WP_002365866 | hypothetical protein | - |
| CEQ02_RS00250 (CEQ02_00250) | 38582..39190 | + | 609 | WP_016616030 | hypothetical protein | - |
| CEQ02_RS00255 (CEQ02_00255) | 39304..40059 | + | 756 | WP_002367687 | Fic family protein | - |
| CEQ02_RS00260 (CEQ02_00260) | 40076..40534 | + | 459 | WP_002370354 | single-stranded DNA-binding protein | - |
| CEQ02_RS00265 (CEQ02_00265) | 40580..40762 | + | 183 | WP_002367689 | hypothetical protein | - |
| CEQ02_RS00270 (CEQ02_00270) | 40804..41289 | + | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| CEQ02_RS00275 (CEQ02_00275) | 41289..43118 | + | 1830 | WP_107164624 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| CEQ02_RS00280 (CEQ02_00280) | 43169..45328 | + | 2160 | WP_107164625 | ArdC-like ssDNA-binding domain-containing protein | - |
| CEQ02_RS00285 (CEQ02_00285) | 45361..45633 | + | 273 | WP_002367930 | LtrD | - |
| CEQ02_RS00290 (CEQ02_00290) | 45879..46235 | + | 357 | WP_002393737 | plasmid mobilization relaxosome protein MobC | - |
| CEQ02_RS00295 (CEQ02_00295) | 46236..47921 | + | 1686 | WP_029888123 | relaxase/mobilization nuclease domain-containing protein | - |
Host bacterium
| ID | 17242 | GenBank | NZ_CP028283 |
| Plasmid name | FDAARGOS_324|unnamed3 | Incompatibility group | - |
| Plasmid size | 49440 bp | Coordinate of oriT [Strand] | 45763..45812 [+] |
| Host baterium | Enterococcus faecalis strain FDAARGOS_324 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |