Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116790
• Name: oriT_2.1523|unnamed1
• Organism: Staphylococcus xylosus strain 2.1523
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP066722 (41984..42108 [-], 125 nt)
• oriT length: 125 nt
• IRs (inverted repeats): 103..108, 116..121 (AGCATA..TATGCT) 86..94, 96..104 (CTATCATTT..AAATGATAG) 69..75, 79..85 (GTCTGGC..GCCAGAC)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 125 nt

>oriT_2.1523|unnamed1
AGTGTCACAAAGGTGAGACAAAAGGGGATTTAAGATATCATTTAAAAATTGACTTGAATTGTTGGAATGTCTGGCTTCGCCAGACCTATCATTTTAAATGATAGCATATTCCCCTTATGCTCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_JGY88_RS13865_2.1523|unnamed1

• ID: 10711
• GenBank: WP_194378946
• Name: mobP2_JGY88_RS13865_2.1523|unnamed1
• UniProt ID: _
• Length: 391 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 391 a.a.
• Molecular weight: 47171.00 Da
• Isoelectric Point: 9.3030

>WP_194378946.1 MULTISPECIES: MobP2 family relaxase [Staphylococcus]
MPKIVTMSEFEQSKNKTKFSKYVDYVDRDEAKKNEHKEFQYDVYTHYMFDEQKSNSMFNDNSNYMSEDEI
KYTKSKFNEAQKNNGIMWKDVISFDNESLKEAGIFDPECKYLDEQKMRQATRKAMQKFEKKEGLENNLVW
SSSIHYNTDNIHVHVAAVEKDVTRERGKRKYSTIKEMKSSFANELFDMSGERTKINNFIRDRIVKGIKEQ
NEPDYDKQMKLQLKKIHEEIKDIPKKEWQYNNNIMKQARPEIDKFTLMYIKNNHGKEFDEFKQDLKKQSK
LYKETYGDKSDYKKYEETKMNDLYSRAGNTVLKQIKSFNEEYNNIKFKEINKQDNKNPLNINPKVNNKMQ
IGYALNSTLYNTQRALRNDFQKERNINQYHYEFDKSYEKEE

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (1-368)

Protein structure

No available structure.

T4CP

t4cp2_JGY88_RS13615_2.1523|unnamed1

• ID: 12510
• GenBank: WP_156981573
• Name: t4cp2_JGY88_RS13615_2.1523|unnamed1
• UniProt ID: _
• Length: 941 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 941 a.a.
• Molecular weight: 108575.68 Da
• Isoelectric Point: 8.3855

>WP_156981573.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus]
MIPFRSKHNHGYHRKKASFSQNKHLLAHPIGLLALLVVGFIFIMLAANFFINMLNNLIFAGGDIFDNMKK
INNEWTNYFFKVRFDLALFYIPFFVLLTVLWGKKVYQIRQKFKSLDNQEKASGRFSTKKEIKQQYKAIAQ
REKQFEGEGGLPVAMFGVYDFATKTKQYLEQRKTAKTEINSMPDLTQDEKYNMKKDKLGQINKEFVSTFF
LKREFTFIDESPTNNIFVGTSRSGKGEVFVLSMIENYSRSSHQPSLVVGDMKGELYSASNETLENRNYHT
EVFNLDQPMESTMSFNLLQLVIEEYQKGDLGEAQEMTKQITHTLTNNEGVEDNEWNLMSAALINAMILAL
CEEALPEHPEKVTLYSVSNMLQTLSVKKFYKQIQIGNQVKVIETSALDEYMEKFPSHSPAKTQYATVEAA
PDKMRSSIIGTALKALQPFTTDTIAKLTSHSSLDLNKLGFPSIIDGFSEPDSKFELNVQVKRETNIGTKY
DEVEGINIGVNEYGYWQYPFKTVLNIDDRIEIVETDEFNEEVKYYFYVTSIDKKGQVTFEAKGDIYSSNV
TIRKFNSFAKPIAIFMVVPDYNSANHGIASILVNQIVFELSKNSQLYTEKQSTHRRVIFHLDEAGNMPQI
PNLSQKVNVSLSRGLRFNFFVQAFSQIKDTYGDAYDAIMDACQNKVFIMATQEQTLKDFSDMIGSQQITV
RSRSGETDSLKSSITENQEERPLLRADELAHLQEGETVVVRNLKRQDNKRRKVMSYPIFNSGKYAMKYRY
QYLADLMDTGKSIIHFRPLLKERCEHRHLQLETTLVDWEKIIENMQAGIENDSNNDIDDLTQSLNKSMGE
KANANNPEVYKEENNKKPVTRQKKVLTLKAQLSAEEFDRLTRRFTKTIRMYEKDGNNVDRLTLYKLKQYL
KLRVRNNDITEEQKNKSINYVKQLIEEAKAK

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (190-459)

• TraM recognition site of TraD and TraG (TraG-D_C) (616-733)

Protein structure

No available structure.

Host bacterium

• ID: 17223
• GenBank: NZ_CP066722
• Plasmid name: 2.1523|unnamed1
• Incompatibility group: -
• Plasmid size: 52837 bp
• Coordinate of oriT [Strand]: 41984..42108 [-]
• Host baterium: Staphylococcus xylosus strain 2.1523

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21