Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116784
• Name: oriT_pCK401E
• Organism: Lactiplantibacillus paraplantarum strain CK401
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP053342 (599..619 [-], 21 nt)
• oriT length: 21 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 21 nt

>oriT_pCK401E
AGTGGGGTGTAAGTGCGCATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_CK401_RS15385_pCK401E

• ID: 10708
• GenBank: WP_171284702
• Name: mobQ_CK401_RS15385_pCK401E
• UniProt ID: _
• Length: 687 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 687 a.a.
• Molecular weight: 80458.24 Da
• Isoelectric Point: 9.6115

>WP_171284702.1 MULTISPECIES: MobQ family relaxase [Lactiplantibacillus]
MAIFHMSFSNISAGKGRSAIAGASYRSGEKLFDQKEGRSYFYARSVMPESFILTPKNAPEWASDREKLWN
EVERKDRRTNSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDQGMVSDVSIHRDHPDNPHAHVMLTNR
PFNPDGTWGIKSKKQYILDDNGKKTYTGTSKYPKSRKILMVDWDKKEKMTEWRHNWAASVNQALEQKNIP
DRISEKSFVEQGIADTPMQHEGINSKRHERKAFNQQVKNYRKSKAGYKNMQEKVVNRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKIYPELDFEQITAAERRELIKETNSEQTIFKGSELTERLMNIRDDLLTRQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSPTEQQKITRAVKDLRAIMAVKQVIKTQYHEVLK
RAFPKGDLDGLPLIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQKQLKGQQAKDDQYRKANIGHYQPLNYKPVSPDY
YLKTAFSNAIMTALYARDEDYERQKQAQGLKETEWEMTKKQRQHQTWNRHEDWGRHL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-241)

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (276-384)

Protein structure

No available structure.

Host bacterium

• ID: 17217
• GenBank: NZ_CP053342
• Plasmid name: pCK401E
• Incompatibility group: -
• Plasmid size: 41380 bp
• Coordinate of oriT [Strand]: 599..619 [-]
• Host baterium: Lactiplantibacillus paraplantarum strain CK401

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -