Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116780
• Name: oriT_pEGM182-2
• Organism: Enterococcus casseliflavus strain EGM182
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP050489 (63..92 [-], 30 nt)
• oriT length: 30 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 30 nt

>oriT_pEGM182-2
AACGTAAGTTAGTGGGTGTAAGTGCGCCCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_EGM182_RS00460_pEGM182-2

• ID: 10706
• GenBank: WP_192415612
• Name: mobQ_EGM182_RS00460_pEGM182-2
• UniProt ID: _
• Length: 661 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 661 a.a.
• Molecular weight: 77438.63 Da
• Isoelectric Point: 9.5528

>WP_192415612.1 MobQ family relaxase [Enterococcus casseliflavus]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEYLDRQTL
WNKMELSEKQPNARLCRELNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQEKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKEKNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR

Protein domains

Predicted by InterproScan.

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (289-394)

• MobA/MobL family (MobA_MobL) (18-242)

Protein structure

No available structure.

Host bacterium

• ID: 17213
• GenBank: NZ_CP050489
• Plasmid name: pEGM182-2
• Incompatibility group: -
• Plasmid size: 73709 bp
• Coordinate of oriT [Strand]: 63..92 [-]
• Host baterium: Enterococcus casseliflavus strain EGM182

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -