Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116775
• Name: oriT_pWCZ
• Organism: Lacticaseibacillus paracasei
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_019669 (1590..1626 [+], 37 nt)
• oriT length: 37 nt
• IRs (inverted repeats): 14..21, 28..35 (AAAGTATA..TATACTTT) 1..6, 13..18 (ACTTTG..CAAAGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 37 nt

>oriT_pWCZ
ACTTTGTGATAGCAAAGTATACTGGGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_HTA42_RS00015_pWCZ

• ID: 10702
• GenBank: WP_015092849
• Name: mobV_HTA42_RS00015_pWCZ
• UniProt ID: K7YJX6
• Length: 436 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 436 a.a.
• Molecular weight: 50731.93 Da
• Isoelectric Point: 7.0626

>WP_015092849.1 MobV family relaxase [Lacticaseibacillus paracasei]
MSQLAAHMQKFKIGNLGGLQRHDERTLQHHSNPDIDVSKSLDNFSVLPPERLDRSRSLHKQVQEIIADER
VSNRAVRKDAVVLAEWVISSDSAFFEGLSSDETERFFTDAYQWFANHFGADHIPYATVHMDETTPHMHMG
VIPLTNGRLSAKTIFNREALRFIQADLPQKLNEKGWNIERGERGSKRKHLSIERYKEVAESLKQGEPLFD
VSKLEHERVPIVNMFNFVKGKEATQNYILPPDDYQKLTERVRGFSDLGHAVKDVNDLKAALNARESLIRQ
REKELDKREDAISKNENLIETYDFWYDQAYEQSDKVTQLQGQIKTLKAENKQWQQHYYKLVDAVHDMAKT
IGTLIADYPAWEQKLNDFGQRVIRGTRAFAKNTLNKVQAYGAAEDIDTTVEISKNIEPDMYPEREQERRQ
RLQQQRKRQQQQGLER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (4-197)

Protein structure

• AlphaFold DB: K7YJX6

Host bacterium

• ID: 17208
• GenBank: NC_019669
• Plasmid name: pWCZ
• Incompatibility group: -
• Plasmid size: 3078 bp
• Coordinate of oriT [Strand]: 1590..1626 [+]
• Host baterium: Lacticaseibacillus paracasei

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -