Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 116767 |
Name | oriT_p602815-CTXM |
Organism | Raoultella ornithinolytica strain 170602815 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_MZ753460 (106176..106224 [+], 49 nt) |
oriT length | 49 nt |
IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
Location of nic site | 32..33 |
Conserved sequence flanking the nic site |
TGTGTGGTGA |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_p602815-CTXM
AATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 10696 | GenBank | WP_063160318 |
Name | traI_NLJ19_RS00385_p602815-CTXM | UniProt ID | _ |
Length | 1752 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190491.29 Da Isoelectric Point: 6.2578
>WP_063160318.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQIFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSENILANRIAFGKIYQNTLRADVES
LGYRTVDAGKNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTSDASVVRQAPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHKTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAVD
SQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VAHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPDSLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADIAIMKEIVRQVPELRPAVYSLIERDVHHALTTIEQVTPEQVPRKEGVWAPGSSVVEFTPTQEKAIQK
ALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKTTRTLTPKADQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQIFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSENILANRIAFGKIYQNTLRADVES
LGYRTVDAGKNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTSDASVVRQAPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHKTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAVD
SQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VAHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
AQIRSGQLLNVPVAPGRGNDLLISRQAWDAEKSILTRVLEGKDAVAPLMDRVPDSLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADIAIMKEIVRQVPELRPAVYSLIERDVHHALTTIEQVTPEQVPRKEGVWAPGSSVVEFTPTQEKAIQK
ALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKTTRTLTPKADQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 6298 | GenBank | WP_004197733 |
Name | WP_004197733_p602815-CTXM | UniProt ID | A0A0E3KB00 |
Length | 129 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 129 a.a. Molecular weight: 14835.90 Da Isoelectric Point: 4.6012
>WP_004197733.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Bacteria]
MGKVQAYPSDEVCEKINAIVEKRRMEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNRAL
LESVVKTQFSVNKILGIECLSPHVKDDPRWQWKSMVLNIQEDVQEAMRNFFPDEDAEGE
MGKVQAYPSDEVCEKINAIVEKRRMEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNRAL
LESVVKTQFSVNKILGIECLSPHVKDDPRWQWKSMVLNIQEDVQEAMRNFFPDEDAEGE
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0E3KB00 |
T4CP
ID | 12496 | GenBank | WP_009309880 |
Name | traD_NLJ19_RS00390_p602815-CTXM | UniProt ID | _ |
Length | 769 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85571.58 Da Isoelectric Point: 5.1733
>WP_009309880.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPDDAGSHAGEQPEPVSQPA
PAVMTVTPAPVKSPPTTKRPAAEPSARTAEPPVLRVTTVPLIKPKAAAAAGTAATASSAGTPAAAAGGTE
QVLAQQSAEQGQAMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDLEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPDDAGSHAGEQPEPVSQPA
PAVMTVTPAPVKSPPTTKRPAAEPSARTAEPPVLRVTTVPLIKPKAAAAAGTAATASSAGTPAAAAGGTE
QVLAQQSAEQGQAMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDLEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 76639..106782
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
NLJ19_RS00390 | 76639..78948 | - | 2310 | WP_009309880 | type IV conjugative transfer system coupling protein TraD | virb4 |
NLJ19_RS00395 | 79074..79766 | - | 693 | WP_009309879 | hypothetical protein | - |
NLJ19_RS00400 | 80501..83374 | - | 2874 | WP_009309878 | conjugal transfer mating-pair stabilization protein TraG | traG |
NLJ19_RS00405 | 83374..84744 | - | 1371 | WP_009309877 | conjugal transfer pilus assembly protein TraH | traH |
NLJ19_RS00410 | 84731..85159 | - | 429 | WP_004152689 | hypothetical protein | - |
NLJ19_RS00415 | 85152..85724 | - | 573 | WP_063160290 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
NLJ19_RS00420 | 85696..85935 | - | 240 | WP_009309875 | type-F conjugative transfer system pilin chaperone TraQ | - |
NLJ19_RS00425 | 85946..86698 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
NLJ19_RS00430 | 86719..87045 | - | 327 | WP_004194451 | hypothetical protein | - |
NLJ19_RS00435 | 87058..87306 | - | 249 | WP_004152675 | hypothetical protein | - |
NLJ19_RS00440 | 87284..87538 | - | 255 | WP_004195500 | conjugal transfer protein TrbE | - |
NLJ19_RS00445 | 87570..89525 | - | 1956 | WP_148866277 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
NLJ19_RS00450 | 89584..90231 | - | 648 | WP_039698503 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
NLJ19_RS00455 | 90375..90977 | - | 603 | WP_022631520 | hypothetical protein | - |
NLJ19_RS00460 | 91271..91723 | + | 453 | WP_172686240 | hypothetical protein | - |
NLJ19_RS00465 | 91698..92246 | - | 549 | WP_022631518 | hypothetical protein | - |
NLJ19_RS00470 | 92261..93244 | - | 984 | WP_223176982 | conjugal transfer pilus assembly protein TraU | traU |
NLJ19_RS00475 | 93264..93890 | - | 627 | WP_020314628 | type-F conjugative transfer system protein TraW | traW |
NLJ19_RS00480 | 93890..94282 | - | 393 | WP_080816432 | type-F conjugative transfer system protein TrbI | - |
NLJ19_RS00485 | 94279..96918 | - | 2640 | WP_042946320 | type IV secretion system protein TraC | virb4 |
NLJ19_RS00490 | 96990..97388 | - | 399 | WP_074186017 | hypothetical protein | - |
NLJ19_RS00495 | 97396..97686 | - | 291 | WP_023340940 | hypothetical protein | - |
NLJ19_RS00500 | 97683..98087 | - | 405 | WP_042939116 | hypothetical protein | - |
NLJ19_RS00505 | 98154..98465 | - | 312 | WP_023316403 | hypothetical protein | - |
NLJ19_RS00510 | 98466..98684 | - | 219 | WP_023316402 | hypothetical protein | - |
NLJ19_RS00515 | 98708..98992 | - | 285 | WP_023316401 | hypothetical protein | - |
NLJ19_RS00520 | 98997..99407 | - | 411 | WP_020805613 | hypothetical protein | - |
NLJ19_RS00525 | 99539..100108 | - | 570 | WP_023340942 | type IV conjugative transfer system lipoprotein TraV | traV |
NLJ19_RS00530 | 100131..100727 | - | 597 | WP_023340943 | conjugal transfer pilus-stabilizing protein TraP | - |
NLJ19_RS00535 | 100720..102144 | - | 1425 | WP_042946342 | F-type conjugal transfer pilus assembly protein TraB | traB |
NLJ19_RS00540 | 102144..102884 | - | 741 | WP_032738531 | type-F conjugative transfer system secretin TraK | traK |
NLJ19_RS00545 | 102871..103437 | - | 567 | WP_020316627 | type IV conjugative transfer system protein TraE | traE |
NLJ19_RS00550 | 103457..103762 | - | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
NLJ19_RS00555 | 103776..104144 | - | 369 | WP_063267011 | type IV conjugative transfer system pilin TraA | - |
NLJ19_RS00560 | 104206..104370 | - | 165 | WP_032444506 | TraY domain-containing protein | - |
NLJ19_RS00565 | 104562..105236 | - | 675 | WP_004197737 | hypothetical protein | - |
NLJ19_RS00570 | 105475..105864 | - | 390 | WP_004197733 | conjugal transfer relaxosome DNA-binding protein TraM | - |
NLJ19_RS00575 | 106297..106782 | + | 486 | WP_022631514 | transglycosylase SLT domain-containing protein | virB1 |
NLJ19_RS00580 | 106815..107144 | - | 330 | WP_011977736 | DUF5983 family protein | - |
NLJ19_RS00585 | 107177..107998 | - | 822 | WP_004152492 | DUF932 domain-containing protein | - |
NLJ19_RS00590 | 108818..109651 | - | 834 | WP_004152751 | N-6 DNA methylase | - |
NLJ19_RS00595 | 109702..109848 | - | 147 | WP_004152750 | hypothetical protein | - |
NLJ19_RS00600 | 109943..110290 | - | 348 | WP_004152749 | hypothetical protein | - |
NLJ19_RS00605 | 110347..110700 | - | 354 | WP_004152748 | hypothetical protein | - |
NLJ19_RS00610 | 111330..111680 | - | 351 | WP_004153414 | hypothetical protein | - |
Host bacterium
ID | 17200 | GenBank | NZ_MZ753460 |
Plasmid name | p602815-CTXM | Incompatibility group | IncFII |
Plasmid size | 138583 bp | Coordinate of oriT [Strand] | 106176..106224 [+] |
Host baterium | Raoultella ornithinolytica strain 170602815 |
Cargo genes
Drug resistance gene | blaCTX-M-14 |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | AcrIE9 |