Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116765 |
| Name | oriT_p602815-KPC  |
| Organism | Raoultella ornithinolytica strain 170602815 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MZ753458 (37239..37833 [+], 595 nt) |
| oriT length | 595 nt |
| IRs (inverted repeats) | 569..574, 579..584 (ATGCCC..GGGCAT) 497..503, 508..514 (CCTCCCG..CGGGAGG) 433..438, 442..447 (GTTCGC..GCGAAC) 75..80, 86..91 (TGCTTT..AAAGCA) 69..74, 82..87 (TTACTT..AAGTAA) |
| Location of nic site | 294..295 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 595 nt
>oriT_p602815-KPC
TGTTAAATACATTGTAGTTTTGATAAAATCATTAGTATCTGGCATAAAGTTATCATTATCATCTTCTGTTACTTTGCTTTTAAGTAAAGCACTGGCAAGCAATTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACCTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCTAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCTTTGATCTAGCACTAGTCTTGAATTGTGCTTCAGCGTCGAGGAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACCGGAAACATCTCAATGGCTGTGAGCTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTCATGACGTGTGAAACTATTCAGGGTCTGGTGTGTCGCCTACCATGCCCTGACGGGCATCACACAACCAG
TGTTAAATACATTGTAGTTTTGATAAAATCATTAGTATCTGGCATAAAGTTATCATTATCATCTTCTGTTACTTTGCTTTTAAGTAAAGCACTGGCAAGCAATTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACCTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCTAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCTTTGATCTAGCACTAGTCTTGAATTGTGCTTCAGCGTCGAGGAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACCGGAAACATCTCAATGGCTGTGAGCTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTCATGACGTGTGAAACTATTCAGGGTCTGGTGTGTCGCCTACCATGCCCTGACGGGCATCACACAACCAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10694 | GenBank | WP_044715149 |
| Name | mobP1_NLJ08_RS00220_p602815-KPC |
UniProt ID | A0A2L1K6C2 |
| Length | 386 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 386 a.a. Molecular weight: 44400.90 Da Isoelectric Point: 10.6126
>WP_044715149.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVSVDKEYRFKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQNVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKHGVQHGWKTVHRNEWKLDNLGVKGIDRTPSSSKELVLN
SAAMLQKQQLQMKQFIQIKQAMLQNEQKMKIGIKLG
MGVSVDKEYRFKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQNVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKHGVQHGWKTVHRNEWKLDNLGVKGIDRTPSSSKELVLN
SAAMLQKQQLQMKQFIQIKQAMLQNEQKMKIGIKLG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2L1K6C2 |
T4CP
| ID | 12494 | GenBank | WP_044715174 |
| Name | t4cp2_NLJ08_RS00160_p602815-KPC |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69245.98 Da Isoelectric Point: 7.1493
>WP_044715174.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWLFIGFVMCLFTYYAGSVAVYFFNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAMPA
LLSGLAASVIVPVFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVILQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPIFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYTTAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAKKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTKNIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWLFIGFVMCLFTYYAGSVAVYFFNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAMPA
LLSGLAASVIVPVFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVILQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPIFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYTTAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAKKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTKNIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 25769..34669
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NLJ08_RS00135 | 21060..21530 | - | 471 | WP_044715183 | thermonuclease family protein | - |
| NLJ08_RS00140 | 21527..21847 | - | 321 | WP_044715215 | hypothetical protein | - |
| NLJ08_RS00145 | 21868..22071 | - | 204 | WP_044715181 | hypothetical protein | - |
| NLJ08_RS00150 | 22140..22478 | - | 339 | WP_225622178 | hypothetical protein | - |
| NLJ08_RS00155 | 22483..22878 | - | 396 | WP_044715177 | cag pathogenicity island Cag12 family protein | - |
| NLJ08_RS00160 | 22875..24710 | - | 1836 | WP_044715174 | type IV secretory system conjugative DNA transfer family protein | - |
| NLJ08_RS00165 | 24713..25745 | - | 1033 | Protein_33 | P-type DNA transfer ATPase VirB11 | - |
| NLJ08_RS00170 | 25769..26980 | - | 1212 | WP_044715172 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| NLJ08_RS00175 | 26977..27906 | - | 930 | WP_044715170 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| NLJ08_RS00180 | 27911..28639 | - | 729 | WP_044715168 | virB8 family protein | virB8 |
| NLJ08_RS00185 | 28879..29934 | - | 1056 | WP_044715165 | type IV secretion system protein | virB6 |
| NLJ08_RS00190 | 29946..30203 | - | 258 | WP_044715163 | EexN family lipoprotein | - |
| NLJ08_RS00195 | 30213..30959 | - | 747 | WP_044715162 | type IV secretion system protein | - |
| NLJ08_RS00200 | 30970..33729 | - | 2760 | WP_044715159 | VirB3 family type IV secretion system protein | virb4 |
| NLJ08_RS00205 | 33751..34041 | - | 291 | WP_044715156 | TrbC/VirB2 family protein | virB2 |
| NLJ08_RS00210 | 34025..34669 | - | 645 | WP_044715155 | lytic transglycosylase domain-containing protein | virB1 |
| NLJ08_RS00215 | 34893..35384 | - | 492 | WP_044715152 | transcription termination/antitermination NusG family protein | - |
| NLJ08_RS00220 | 35740..36900 | - | 1161 | WP_044715149 | MobP1 family relaxase | - |
| NLJ08_RS00225 | 36903..37451 | - | 549 | WP_044715148 | DNA distortion polypeptide 1 | - |
| NLJ08_RS00230 | 37779..38036 | - | 258 | WP_044715147 | hypothetical protein | - |
| NLJ08_RS00235 | 38052..38201 | - | 150 | WP_162852120 | hypothetical protein | - |
| NLJ08_RS00240 | 38279..38620 | - | 342 | WP_059347451 | hypothetical protein | - |
| NLJ08_RS00245 | 38717..38956 | - | 240 | WP_044715143 | hypothetical protein | - |
| NLJ08_RS00250 | 38949..39230 | - | 282 | WP_048235114 | hypothetical protein | - |
Host bacterium
| ID | 17198 | GenBank | NZ_MZ753458 |
| Plasmid name | p602815-KPC | Incompatibility group | IncX5 |
| Plasmid size | 41875 bp | Coordinate of oriT [Strand] | 37239..37833 [+] |
| Host baterium | Raoultella ornithinolytica strain 170602815 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |