Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116752 |
| Name | oriT_pSN32  |
| Organism | Staphylococcus equorum strain SN32 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP098666 (2980..3015 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pSN32
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10686 | GenBank | WP_030061449 |
| Name | MobA_MobL_ND724_RS00030_pSN32 |
UniProt ID | A0A848EWD6 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78725.60 Da Isoelectric Point: 5.1613
>WP_030061449.1 MULTISPECIES: MobA/MobL family protein [Staphylococcus]
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYPEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYPEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A848EWD6 |
T4CP
| ID | 12491 | GenBank | WP_030061486 |
| Name | t4cp2_ND724_RS00180_pSN32 |
UniProt ID | _ |
| Length | 548 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 548 a.a. Molecular weight: 62899.75 Da Isoelectric Point: 5.9714
>WP_030061486.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcaceae]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 33048..44651
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ND724_RS00135 | 28103..28237 | - | 135 | WP_254903663 | hypothetical protein | - |
| ND724_RS00140 (ND724_00135) | 28450..28962 | - | 513 | WP_032495402 | MerR family transcriptional regulator | - |
| ND724_RS00145 (ND724_00140) | 28965..30137 | - | 1173 | WP_032495458 | IS256-like element ISEnfa4 family transposase | - |
| ND724_RS00150 (ND724_00145) | 30223..30489 | - | 267 | WP_030061495 | hypothetical protein | - |
| ND724_RS00155 (ND724_00150) | 30558..31172 | - | 615 | WP_030061494 | hypothetical protein | - |
| ND724_RS00160 (ND724_00155) | 31188..31811 | - | 624 | WP_030061493 | hypothetical protein | - |
| ND724_RS00165 (ND724_00160) | 31848..32579 | - | 732 | WP_000869299 | hypothetical protein | - |
| ND724_RS00170 (ND724_00165) | 32636..33031 | - | 396 | WP_000608969 | single-stranded DNA-binding protein | - |
| ND724_RS00175 (ND724_00170) | 33048..33965 | - | 918 | WP_030061488 | conjugal transfer protein TrbL family protein | trsL |
| ND724_RS00180 (ND724_00175) | 34043..35689 | - | 1647 | WP_030061486 | VirD4-like conjugal transfer protein, CD1115 family | - |
| ND724_RS00185 (ND724_00180) | 35686..36150 | - | 465 | WP_000715271 | hypothetical protein | trsJ |
| ND724_RS00190 (ND724_00185) | 36166..38268 | - | 2103 | WP_030061483 | DNA topoisomerase III | - |
| ND724_RS00195 (ND724_00190) | 38284..38769 | - | 486 | WP_000735570 | TrsH/TraH family protein | - |
| ND724_RS00200 (ND724_00195) | 38779..39855 | - | 1077 | WP_031921304 | CHAP domain-containing protein | trsG |
| ND724_RS00205 (ND724_00200) | 39873..41153 | - | 1281 | WP_030061477 | hypothetical protein | trsF |
| ND724_RS00210 (ND724_00205) | 41165..43177 | - | 2013 | WP_030061475 | VirB4 family type IV secretion system protein | virb4 |
| ND724_RS00215 (ND724_00210) | 43198..43881 | - | 684 | WP_030061473 | TrsD/TraD family conjugative transfer protein | trsD |
| ND724_RS00220 (ND724_00215) | 43868..44275 | - | 408 | WP_030061471 | hypothetical protein | trsC |
| ND724_RS00225 (ND724_00220) | 44334..44651 | - | 318 | WP_030061469 | CagC family type IV secretion system protein | virB2 |
| ND724_RS00230 (ND724_00225) | 44668..45642 | - | 975 | WP_030061467 | conjugative transfer protein TrsA | - |
| ND724_RS00235 (ND724_00230) | 45815..46003 | + | 189 | WP_030061465 | hypothetical protein | - |
| ND724_RS00240 (ND724_00235) | 46107..47066 | - | 960 | WP_030061463 | replication initiator protein A | - |
Host bacterium
| ID | 17185 | GenBank | NZ_CP098666 |
| Plasmid name | pSN32 | Incompatibility group | - |
| Plasmid size | 47066 bp | Coordinate of oriT [Strand] | 2980..3015 [-] |
| Host baterium | Staphylococcus equorum strain SN32 |
Cargo genes
| Drug resistance gene | aac(6')-aph(2''), cfr |
| Virulence gene | - |
| Metal resistance gene | mco |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |