Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   116747
Name   oriT_pSN65 in_silico
Organism   Staphylococcus arlettae strain SN65
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP098665 (2980..3015 [-], 36 nt)
oriT length   36 nt
IRs (inverted repeats)      4..10, 14..20  (GCGAACG..CGTTCGC)
Location of nic site      29..30
Conserved sequence flanking the
  nic site  
 
 GTGCGCCCTT
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 36 nt

>oriT_pSN65
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   10681 GenBank   WP_182094133
Name   MobA_MobL_ND725_RS00030_pSN65 insolico UniProt ID   _
Length   665 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 665 a.a.        Molecular weight: 78741.64 Da        Isoelectric Point: 5.1613

>WP_182094133.1 MULTISPECIES: MobA/MobL family protein [Staphylococcaceae]
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYLEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL

  Protein domains


Predicted by InterproScan.

(17-216)


  Protein structure



No available structure.




T4CP


ID   12486 GenBank   WP_030061486
Name   t4cp2_ND725_RS00180_pSN65 insolico UniProt ID   _
Length   548 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 548 a.a.        Molecular weight: 62899.75 Da        Isoelectric Point: 5.9714

>WP_030061486.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcaceae]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK

  Protein domains


Predicted by InterproScan.

(3-462)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 32151..43754

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND725_RS00150 (ND725_00145) 28445..29530 - 1086 WP_031883924 tyrosine-type recombinase/integrase -
ND725_RS00155 (ND725_00150) 29658..30275 - 618 WP_182094135 hypothetical protein -
ND725_RS00160 (ND725_00155) 30291..30914 - 624 WP_030061493 hypothetical protein -
ND725_RS00165 (ND725_00160) 30951..31682 - 732 WP_000869299 hypothetical protein -
ND725_RS00170 (ND725_00165) 31739..32134 - 396 WP_000608969 single-stranded DNA-binding protein -
ND725_RS00175 (ND725_00170) 32151..33068 - 918 WP_030061488 conjugal transfer protein TrbL family protein trsL
ND725_RS00180 (ND725_00175) 33146..34792 - 1647 WP_030061486 VirD4-like conjugal transfer protein, CD1115 family -
ND725_RS00185 (ND725_00180) 34789..35253 - 465 WP_000715271 hypothetical protein trsJ
ND725_RS00190 (ND725_00185) 35269..37371 - 2103 WP_030061483 DNA topoisomerase III -
ND725_RS00195 (ND725_00190) 37387..37872 - 486 WP_000735570 TrsH/TraH family protein -
ND725_RS00200 (ND725_00195) 37882..38958 - 1077 WP_031921304 CHAP domain-containing protein trsG
ND725_RS00205 (ND725_00200) 38976..40256 - 1281 WP_030061477 hypothetical protein trsF
ND725_RS00210 (ND725_00205) 40268..42280 - 2013 WP_030061475 VirB4 family type IV secretion system protein virb4
ND725_RS00215 (ND725_00210) 42301..42984 - 684 WP_030061473 TrsD/TraD family conjugative transfer protein trsD
ND725_RS00220 (ND725_00215) 42971..43378 - 408 WP_030061471 hypothetical protein trsC
ND725_RS00225 (ND725_00220) 43437..43754 - 318 WP_030061469 CagC family type IV secretion system protein virB2
ND725_RS00230 (ND725_00225) 43771..44745 - 975 WP_030061467 conjugative transfer protein TrsA -
ND725_RS00235 (ND725_00230) 44918..45106 + 189 WP_030061465 hypothetical protein -
ND725_RS00240 (ND725_00235) 45210..46169 - 960 WP_030061463 replication initiator protein A -


Host bacterium


ID   17180 GenBank   NZ_CP098665
Plasmid name   pSN65 Incompatibility group   -
Plasmid size   46169 bp Coordinate of oriT [Strand]   2980..3015 [-]
Host baterium   Staphylococcus arlettae strain SN65

Cargo genes


Drug resistance gene   aac(6')-aph(2''), cfr, fexA
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21