Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116736 |
| Name | oriT_pTZ360  |
| Organism | Staphylococcus cohnii strain TZ360 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP098652 (2980..3015 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pTZ360
CACGCGATCGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGATCGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10670 | GenBank | WP_030061449 |
| Name | MobA_MobL_ND738_RS00030_pTZ360 |
UniProt ID | A0A848EWD6 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78725.60 Da Isoelectric Point: 5.1613
>WP_030061449.1 MULTISPECIES: MobA/MobL family protein [Staphylococcus]
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYPEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYPEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A848EWD6 |
T4CP
| ID | 12476 | GenBank | WP_030061486 |
| Name | t4cp2_ND738_RS00150_pTZ360 |
UniProt ID | _ |
| Length | 548 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 548 a.a. Molecular weight: 62899.75 Da Isoelectric Point: 5.9714
>WP_030061486.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcaceae]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 25486..37089
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ND738_RS00105 | 20541..20675 | - | 135 | WP_254903663 | hypothetical protein | - |
| ND738_RS00110 (ND738_00105) | 20888..21400 | - | 513 | WP_032495402 | MerR family transcriptional regulator | - |
| ND738_RS00115 (ND738_00110) | 21403..22575 | - | 1173 | WP_032495458 | IS256-like element ISEnfa4 family transposase | - |
| ND738_RS00120 (ND738_00115) | 22661..22927 | - | 267 | WP_030061495 | hypothetical protein | - |
| ND738_RS00125 (ND738_00120) | 22996..23610 | - | 615 | WP_030061494 | hypothetical protein | - |
| ND738_RS00130 (ND738_00125) | 23626..24249 | - | 624 | WP_030061493 | hypothetical protein | - |
| ND738_RS00135 (ND738_00130) | 24286..25017 | - | 732 | WP_000869299 | hypothetical protein | - |
| ND738_RS00140 (ND738_00135) | 25074..25469 | - | 396 | WP_000608969 | single-stranded DNA-binding protein | - |
| ND738_RS00145 (ND738_00140) | 25486..26403 | - | 918 | WP_030061488 | conjugal transfer protein TrbL family protein | trsL |
| ND738_RS00150 (ND738_00145) | 26481..28127 | - | 1647 | WP_030061486 | VirD4-like conjugal transfer protein, CD1115 family | - |
| ND738_RS00155 (ND738_00150) | 28124..28588 | - | 465 | WP_000715271 | hypothetical protein | trsJ |
| ND738_RS00160 (ND738_00155) | 28604..30706 | - | 2103 | WP_030061483 | DNA topoisomerase III | - |
| ND738_RS00165 (ND738_00160) | 30722..31207 | - | 486 | WP_000735570 | TrsH/TraH family protein | - |
| ND738_RS00170 (ND738_00165) | 31217..32293 | - | 1077 | WP_030061480 | CHAP domain-containing protein | trsG |
| ND738_RS00175 (ND738_00170) | 32311..33591 | - | 1281 | WP_030061477 | hypothetical protein | trsF |
| ND738_RS00180 (ND738_00175) | 33603..35615 | - | 2013 | WP_030061475 | VirB4 family type IV secretion system protein | virb4 |
| ND738_RS00185 (ND738_00180) | 35636..36319 | - | 684 | WP_030061473 | TrsD/TraD family conjugative transfer protein | trsD |
| ND738_RS00190 (ND738_00185) | 36306..36713 | - | 408 | WP_030061471 | hypothetical protein | trsC |
| ND738_RS00195 (ND738_00190) | 36772..37089 | - | 318 | WP_030061469 | CagC family type IV secretion system protein | virB2 |
| ND738_RS00200 (ND738_00195) | 37106..38080 | - | 975 | WP_030061467 | conjugative transfer protein TrsA | - |
| ND738_RS00205 (ND738_00200) | 38253..38441 | + | 189 | WP_030061465 | hypothetical protein | - |
| ND738_RS00210 (ND738_00205) | 38545..39504 | - | 960 | WP_030061463 | replication initiator protein A | - |
Host bacterium
| ID | 17169 | GenBank | NZ_CP098652 |
| Plasmid name | pTZ360 | Incompatibility group | - |
| Plasmid size | 39504 bp | Coordinate of oriT [Strand] | 2980..3015 [-] |
| Host baterium | Staphylococcus cohnii strain TZ360 |
Cargo genes
| Drug resistance gene | aac(6')-aph(2''), cfr |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |