Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   116736
Name   oriT_pTZ360 in_silico
Organism   Staphylococcus cohnii strain TZ360
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP098652 (2980..3015 [-], 36 nt)
oriT length   36 nt
IRs (inverted repeats)     _
Location of nic site      29..30
Conserved sequence flanking the
  nic site  
 
 GTGCGCCCTT
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 36 nt

>oriT_pTZ360
CACGCGATCGGAACGTTCGCATAAGTGCGCCCTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   10670 GenBank   WP_030061449
Name   MobA_MobL_ND738_RS00030_pTZ360 insolico UniProt ID   A0A848EWD6
Length   665 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 665 a.a.        Molecular weight: 78725.60 Da        Isoelectric Point: 5.1613

>WP_030061449.1 MULTISPECIES: MobA/MobL family protein [Staphylococcus]
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYPEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL

  Protein domains


Predicted by InterproScan.

(17-216)

(265-359)


  Protein structure


Source ID Structure
AlphaFold DB A0A848EWD6


T4CP


ID   12476 GenBank   WP_030061486
Name   t4cp2_ND738_RS00150_pTZ360 insolico UniProt ID   _
Length   548 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 548 a.a.        Molecular weight: 62899.75 Da        Isoelectric Point: 5.9714

>WP_030061486.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcaceae]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK

  Protein domains


Predicted by InterproScan.

(3-462)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 25486..37089

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND738_RS00105 20541..20675 - 135 WP_254903663 hypothetical protein -
ND738_RS00110 (ND738_00105) 20888..21400 - 513 WP_032495402 MerR family transcriptional regulator -
ND738_RS00115 (ND738_00110) 21403..22575 - 1173 WP_032495458 IS256-like element ISEnfa4 family transposase -
ND738_RS00120 (ND738_00115) 22661..22927 - 267 WP_030061495 hypothetical protein -
ND738_RS00125 (ND738_00120) 22996..23610 - 615 WP_030061494 hypothetical protein -
ND738_RS00130 (ND738_00125) 23626..24249 - 624 WP_030061493 hypothetical protein -
ND738_RS00135 (ND738_00130) 24286..25017 - 732 WP_000869299 hypothetical protein -
ND738_RS00140 (ND738_00135) 25074..25469 - 396 WP_000608969 single-stranded DNA-binding protein -
ND738_RS00145 (ND738_00140) 25486..26403 - 918 WP_030061488 conjugal transfer protein TrbL family protein trsL
ND738_RS00150 (ND738_00145) 26481..28127 - 1647 WP_030061486 VirD4-like conjugal transfer protein, CD1115 family -
ND738_RS00155 (ND738_00150) 28124..28588 - 465 WP_000715271 hypothetical protein trsJ
ND738_RS00160 (ND738_00155) 28604..30706 - 2103 WP_030061483 DNA topoisomerase III -
ND738_RS00165 (ND738_00160) 30722..31207 - 486 WP_000735570 TrsH/TraH family protein -
ND738_RS00170 (ND738_00165) 31217..32293 - 1077 WP_030061480 CHAP domain-containing protein trsG
ND738_RS00175 (ND738_00170) 32311..33591 - 1281 WP_030061477 hypothetical protein trsF
ND738_RS00180 (ND738_00175) 33603..35615 - 2013 WP_030061475 VirB4 family type IV secretion system protein virb4
ND738_RS00185 (ND738_00180) 35636..36319 - 684 WP_030061473 TrsD/TraD family conjugative transfer protein trsD
ND738_RS00190 (ND738_00185) 36306..36713 - 408 WP_030061471 hypothetical protein trsC
ND738_RS00195 (ND738_00190) 36772..37089 - 318 WP_030061469 CagC family type IV secretion system protein virB2
ND738_RS00200 (ND738_00195) 37106..38080 - 975 WP_030061467 conjugative transfer protein TrsA -
ND738_RS00205 (ND738_00200) 38253..38441 + 189 WP_030061465 hypothetical protein -
ND738_RS00210 (ND738_00205) 38545..39504 - 960 WP_030061463 replication initiator protein A -


Host bacterium


ID   17169 GenBank   NZ_CP098652
Plasmid name   pTZ360 Incompatibility group   -
Plasmid size   39504 bp Coordinate of oriT [Strand]   2980..3015 [-]
Host baterium   Staphylococcus cohnii strain TZ360

Cargo genes


Drug resistance gene   aac(6')-aph(2''), cfr
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21