Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116728 |
| Name | oriT_pSN134  |
| Organism | Staphylococcus saprophyticus strain SN134 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP098664 (2980..3015 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pSN134
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10666 | GenBank | WP_182094133 |
| Name | MobA_MobL_ND726_RS00030_pSN134 |
UniProt ID | _ |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78741.64 Da Isoelectric Point: 5.1613
>WP_182094133.1 MULTISPECIES: MobA/MobL family protein [Staphylococcaceae]
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYLEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKAAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVNDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINDENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKDKHNEWRKRWETVQNKHLEMNGFSVRVSADSYKKQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKINKMFDEVDDMKSHKLNAFSYMDKSDSTTLKDMAKDLKIYV
TPINMYEENERLYDLKQKTALITDDEDRLNKIENIEDRQKKLESINEVFEKQAGIFFDKNYLEQALNYSD
DEKIFITRTILNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISIESIEKESNFFADKLSNILDK
NNLSFDDVLENKHEGIEDSLKIDYYTSKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLNEY
DFDYSDNNDLKHLYQESNEVGDETSKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLVNAGLDSMIFNFN
EIFRERMPKYINNQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12472 | GenBank | WP_030061486 |
| Name | t4cp2_ND726_RS00180_pSN134 |
UniProt ID | _ |
| Length | 548 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 548 a.a. Molecular weight: 62899.75 Da Isoelectric Point: 5.9714
>WP_030061486.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcaceae]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSFKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGYDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKMNKDVWYKSSVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNHDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKIKQQKLEEYKEEQ
RQKELEEEQQAQEEQQNEEVKESKSKNEEQQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 32151..43754
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ND726_RS00150 (ND726_00145) | 28445..29530 | - | 1086 | WP_031883924 | tyrosine-type recombinase/integrase | - |
| ND726_RS00155 (ND726_00150) | 29658..30275 | - | 618 | WP_182094135 | hypothetical protein | - |
| ND726_RS00160 (ND726_00155) | 30291..30914 | - | 624 | WP_030061493 | hypothetical protein | - |
| ND726_RS00165 (ND726_00160) | 30951..31682 | - | 732 | WP_000869299 | hypothetical protein | - |
| ND726_RS00170 (ND726_00165) | 31739..32134 | - | 396 | WP_000608969 | single-stranded DNA-binding protein | - |
| ND726_RS00175 (ND726_00170) | 32151..33068 | - | 918 | WP_030061488 | conjugal transfer protein TrbL family protein | trsL |
| ND726_RS00180 (ND726_00175) | 33146..34792 | - | 1647 | WP_030061486 | VirD4-like conjugal transfer protein, CD1115 family | - |
| ND726_RS00185 (ND726_00180) | 34789..35253 | - | 465 | WP_000715271 | hypothetical protein | trsJ |
| ND726_RS00190 (ND726_00185) | 35269..37371 | - | 2103 | WP_030061483 | DNA topoisomerase III | - |
| ND726_RS00195 (ND726_00190) | 37387..37872 | - | 486 | WP_000735570 | TrsH/TraH family protein | - |
| ND726_RS00200 (ND726_00195) | 37882..38958 | - | 1077 | WP_031921304 | CHAP domain-containing protein | trsG |
| ND726_RS00205 (ND726_00200) | 38976..40256 | - | 1281 | WP_030061477 | hypothetical protein | trsF |
| ND726_RS00210 (ND726_00205) | 40268..42280 | - | 2013 | WP_030061475 | VirB4 family type IV secretion system protein | virb4 |
| ND726_RS00215 (ND726_00210) | 42301..42984 | - | 684 | WP_030061473 | TrsD/TraD family conjugative transfer protein | trsD |
| ND726_RS00220 (ND726_00215) | 42971..43378 | - | 408 | WP_030061471 | hypothetical protein | trsC |
| ND726_RS00225 (ND726_00220) | 43437..43754 | - | 318 | WP_030061469 | CagC family type IV secretion system protein | virB2 |
| ND726_RS00230 (ND726_00225) | 43771..44745 | - | 975 | WP_030061467 | conjugative transfer protein TrsA | - |
| ND726_RS00235 (ND726_00230) | 44918..45106 | + | 189 | WP_030061465 | hypothetical protein | - |
| ND726_RS00240 (ND726_00235) | 45210..46169 | - | 960 | WP_030061463 | replication initiator protein A | - |
Host bacterium
| ID | 17161 | GenBank | NZ_CP098664 |
| Plasmid name | pSN134 | Incompatibility group | - |
| Plasmid size | 46169 bp | Coordinate of oriT [Strand] | 2980..3015 [-] |
| Host baterium | Staphylococcus saprophyticus strain SN134 |
Cargo genes
| Drug resistance gene | aac(6')-aph(2''), cfr, fexA |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |