Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116711 |
| Name | oriT_pUC073C  |
| Organism | Lactococcus cremoris strain UC073 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP068701 (33228..33364 [+], 137 nt) |
| oriT length | 137 nt |
| IRs (inverted repeats) | 21..28, 42..49 (AAAAGGGG..CCCCTTTT) 25..30, 39..44 (GGGGAA..TTCCCC) |
| Location of nic site | 104..105 |
| Conserved sequence flanking the nic site |
GCTTGCAGTA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 137 nt
>oriT_pUC073C
CGACACACTCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGGTTTATATTTTCTATTTTGTT
CGACACACTCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGGTTTATATTTTCTATTTTGTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10658 | GenBank | WP_014573492 |
| Name | Rep_trans_LLUC073_RS13575_pUC073C |
UniProt ID | _ |
| Length | 390 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 390 a.a. Molecular weight: 45660.50 Da Isoelectric Point: 8.9630
>WP_014573492.1 MULTISPECIES: replication initiation factor domain-containing protein [Lactococcus]
MQKYKIDWLSFSVKNYDEEPTNTLDENLLELLGYDFADEFNDISGKNFYNRGATLGNYVNMFWNDYTDTK
NPVRRNSSATMNVVFTGQGSTDLALRNDNDWIQIFKILTTYEPKIKPKDEAEAKPSVNITRIDIALDDYD
ELVKFDDIEKKLQKGHYRSSKRSYNIVKTSDQTGQKLGETIYIGNARTASGSRGNTYMRMYDKLAQYLDK
NQLVTSDVREYWKKTGKKTWQRYEISFSKGYTNSIVEDILKGESIDKIFKTSLRNLIEILTPRGTDTNKN
RWYKTKWWEDFLKYDERKDFDLPNRDVMLAETLEWLRVAVLPSLNVLEKIGQERGFDIYDLLRSAKKPNE
LSKKQSRIIADTRNKSDEKINDYLAKFLLGIKIEKERGGE
MQKYKIDWLSFSVKNYDEEPTNTLDENLLELLGYDFADEFNDISGKNFYNRGATLGNYVNMFWNDYTDTK
NPVRRNSSATMNVVFTGQGSTDLALRNDNDWIQIFKILTTYEPKIKPKDEAEAKPSVNITRIDIALDDYD
ELVKFDDIEKKLQKGHYRSSKRSYNIVKTSDQTGQKLGETIYIGNARTASGSRGNTYMRMYDKLAQYLDK
NQLVTSDVREYWKKTGKKTWQRYEISFSKGYTNSIVEDILKGESIDKIFKTSLRNLIEILTPRGTDTNKN
RWYKTKWWEDFLKYDERKDFDLPNRDVMLAETLEWLRVAVLPSLNVLEKIGQERGFDIYDLLRSAKKPNE
LSKKQSRIIADTRNKSDEKINDYLAKFLLGIKIEKERGGE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12460 | GenBank | WP_014573491 |
| Name | tcpA_LLUC073_RS13580_pUC073C |
UniProt ID | _ |
| Length | 448 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 448 a.a. Molecular weight: 50009.12 Da Isoelectric Point: 9.2108
>WP_014573491.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Lactococcus]
MAVIYKKNAQNVYKLPLYAQFWTWWALLLALAVMSLLLGLVVSIKLVQVVLWSLVAMLTIWRAYSLTKSV
IKAKTIGKYITQKKAEKAVTKSLLATMSVNRLQDTPFISVPSVTVCDSRPSYISVEVEKLAGMYDIDKLT
EDINSSFRGKLGAYAVTSAMITTDGLFYKFVLADVSTDKTWRPATLEDINVEKYIIKLQKGLEVNLSERA
HIAVWGTTGSKKTTVLFGIILQLFSMGADVRFIDGKDEFCSFKGFYPADKIVSDIDPVFEQLEDILAIIK
ERQQIMANEVQKRQKIGLKASEVGLQPVVLVADEIGSIVALMDSKQSKKFVADLTAIIQRGRSVGVSVIA
STQDPSTDTLPQKIRQQFSTKILLGSANADIQRMAFGEVATSGNVEDFRGFYTCDGLTNQPLKFYVCDLY
SHGFNELEAFERAYKIGFQSDEHKKQTR
MAVIYKKNAQNVYKLPLYAQFWTWWALLLALAVMSLLLGLVVSIKLVQVVLWSLVAMLTIWRAYSLTKSV
IKAKTIGKYITQKKAEKAVTKSLLATMSVNRLQDTPFISVPSVTVCDSRPSYISVEVEKLAGMYDIDKLT
EDINSSFRGKLGAYAVTSAMITTDGLFYKFVLADVSTDKTWRPATLEDINVEKYIIKLQKGLEVNLSERA
HIAVWGTTGSKKTTVLFGIILQLFSMGADVRFIDGKDEFCSFKGFYPADKIVSDIDPVFEQLEDILAIIK
ERQQIMANEVQKRQKIGLKASEVGLQPVVLVADEIGSIVALMDSKQSKKFVADLTAIIQRGRSVGVSVIA
STQDPSTDTLPQKIRQQFSTKILLGSANADIQRMAFGEVATSGNVEDFRGFYTCDGLTNQPLKFYVCDLY
SHGFNELEAFERAYKIGFQSDEHKKQTR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 17144 | GenBank | NZ_CP068701 |
| Plasmid name | pUC073C | Incompatibility group | - |
| Plasmid size | 34364 bp | Coordinate of oriT [Strand] | 33228..33364 [+] |
| Host baterium | Lactococcus cremoris strain UC073 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |