Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116706
Name   oriT_A7|unnamed1 in_silico
Organism   Salmonella sp. A7
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP084002 (17303..19016 [-], 1714 nt)
oriT length   1714 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1714 nt

>oriT_A7|unnamed1
GCCGTATGAATGACATTTATTTCCGTCCAATTCATCGAAATCAGTATTTTGAGGAAATGAAAGCATGATAAAACAAATTAAAGTAATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCACCGCCTCCCGTTCCTGTAGAGTGGGATAAAGCCGCCGCCCCATTAAATACCAGTTTACCGCAATGGAGCGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACAGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCCGTATTTTACGCAGTCGCCCACTCAACACGAATTGTCGTCACTGCACAATCCGGCACTAATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGCGCAAAAGGTGTTATTGAATACCAGCCTGTCTTTAATTGTCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCTAATCAATTTAAGTAATACAACCTAATAAGGACTTACCATGAATAAAACCGTTATCGCGGCGGCTGTATTATGTATGGCCGCAGGAACCCCAATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCAACGGGTAACAGTGGTGGAAGTGAATGCCGCTCAGCAGAAAAGGCTTTTTTTAATATAGTCAAAAAGAATAAGCATGGTTTCTTACCTAACCATACGTTGAATGCGAGAAAAGCATTTCTTGGTGAATGTCCCAGCGCAGACCCGGAGGCAGTAAATAAAATACTGTCTAAGTTCGGCAAGGTCAGAGGATAGTAATAATCCGCATAACACCCCCTGTTTACTGTAAAAGGTACAACATGCAAAAAGAAGACTTATTTATCCGAAATATACATTCTCGTAATCAGGACAGGATATCGGTAGCCCTTGTATATGACACGTTATCTAAAGAAGCCCACAGTGGTTGTGGGCTGTATTATGAGATATACGAAAGCAGGCTTATCGGTCTTTTGCGCGATCATCTTTCAGTACTTAATGAAGCTGACGCAAATAAATTAAGACATTATGCCGAAAGCAAAGGCGCGAAAATTGATGATGCGTCCTACAGTGACGCTCTGGAAGCTGAAAGGGAGTGCAGGGCTGAGATATACCGCGAGCAGATGTAATCACACAAAACAAGAGTGTATTGTACAACACACAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGCTATATTTCCCGCCCCAGCGGGGAATATGCCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCGCGACGGTGTGCCGCCTTTGACTTTGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGACACATGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   12456 GenBank   WP_053271849
Name   t4cp2_LCS67_RS22595_A7|unnamed1 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103299.32 Da        Isoelectric Point: 7.6331

>WP_053271849.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacteriaceae]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDETLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHQDSGNPYADEVSRLYYASVGSFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALARYGARSLGTFTEGKMVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEIKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGYAMALMRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEINIRALGGRYYRVISGEPTGWNPFSLPATKRNINFIKQLMKILC
TRNGATITPRDERRLNDAVNAVMSDEPEYRRFGITRLREQLPEPATKEAQENGLDIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(534-813)

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

  Protein structure



No available structure.



ID   12457 GenBank   WP_225090918
Name   t4cp2_LCS67_RS22680_A7|unnamed1 insolico UniProt ID   _
Length   578 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 578 a.a.        Molecular weight: 65743.97 Da        Isoelectric Point: 7.4792

>WP_225090918.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFFGELEEIALVNSINAKNGFSL
KEIFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QTTPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(336-537)

Helicase HerA, central domain (DUF87)

(174-280)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 86..21558

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCS67_RS22570 (LCS67_22570) 1..22421 - 22421 WP_077816599 P-type conjugative transfer protein VirB9 -
LCS67_RS22575 (LCS67_22575) 86..769 - 684 WP_053271846 type IV secretion system protein virB8
LCS67_RS22580 (LCS67_22580) 991..2061 - 1071 WP_088731843 type IV secretion system protein virB6
LCS67_RS22585 (LCS67_22585) 2073..2297 - 225 WP_053271848 EexN family lipoprotein -
LCS67_RS22590 (LCS67_22590) 2309..3010 - 702 WP_040200438 type IV secretion system protein -
LCS67_RS22595 (LCS67_22595) 3028..5766 - 2739 WP_053271849 VirB3 family type IV secretion system protein virb4
LCS67_RS22600 (LCS67_22600) 5779..6084 - 306 WP_012457109 TrbC/VirB2 family protein virB2
LCS67_RS22605 (LCS67_22605) 6081..6791 - 711 WP_004178175 lytic transglycosylase domain-containing protein virB1
LCS67_RS22755 6790..6912 + 123 WP_259320733 hypothetical protein -
LCS67_RS22610 (LCS67_22610) 6909..7127 - 219 WP_053271851 hypothetical protein -
LCS67_RS22615 (LCS67_22615) 7132..7347 - 216 WP_004178170 hypothetical protein -
LCS67_RS22620 (LCS67_22620) 7419..7874 - 456 WP_053271852 transcription termination/antitermination NusG family protein -
LCS67_RS22625 (LCS67_22625) 8125..8481 + 357 WP_011172003 helix-turn-helix transcriptional regulator -
LCS67_RS22630 (LCS67_22630) 8942..9643 + 702 WP_263631173 RepA family replication protein -
LCS67_RS22635 (LCS67_22635) 10506..10835 + 330 WP_000493286 type II toxin-antitoxin system RelE/ParE family toxin -
LCS67_RS22640 (LCS67_22640) 10816..11097 + 282 WP_000780222 helix-turn-helix transcriptional regulator -
LCS67_RS22645 (LCS67_22645) 11475..12257 + 783 WP_004178131 SprT-like domain-containing protein -
LCS67_RS22650 (LCS67_22650) 12377..13024 + 648 WP_225090915 ParA family protein -
LCS67_RS22655 (LCS67_22655) 13065..13298 + 234 WP_004178129 hypothetical protein -
LCS67_RS22660 (LCS67_22660) 13368..13586 + 219 WP_225090916 hypothetical protein -
LCS67_RS22665 (LCS67_22665) 13674..14015 - 342 WP_318248407 hypothetical protein -
LCS67_RS22670 (LCS67_22670) 14076..14549 - 474 WP_045895976 thermonuclease family protein -
LCS67_RS22675 (LCS67_22675) 14586..15341 - 756 WP_023316386 MobC family replication-relaxation protein -
LCS67_RS22680 (LCS67_22680) 15350..17086 - 1737 WP_225090918 type IV secretory system conjugative DNA transfer family protein virb4
LCS67_RS22685 (LCS67_22685) 17866..18171 - 306 WP_225090919 dpoa decarboxylase -
LCS67_RS22690 (LCS67_22690) 18216..18518 - 303 WP_001044654 TrbM/KikA/MpfK family conjugal transfer protein -
LCS67_RS22695 (LCS67_22695) 18554..18952 - 399 WP_072133379 cag pathogenicity island Cag12 family protein -
LCS67_RS22700 (LCS67_22700) 18949..19974 - 1026 WP_000125558 P-type DNA transfer ATPase VirB11 virB11
LCS67_RS22705 (LCS67_22705) 19964..20284 - 321 WP_053271843 hypothetical protein -
LCS67_RS22710 (LCS67_22710) 20296..21558 - 1263 WP_053271844 VirB10/TraB/TrbI family type IV secretion system protein virB10

Host bacterium

ID   17139 GenBank   NZ_CP084002
Plasmid name   A7|unnamed1 Incompatibility group   IncFII
Plasmid size   22421 bp Coordinate of oriT [Strand]   17303..19016 [-]
Host baterium   Salmonella sp. A7

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -