Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116650 |
| Name | oriT_pMAD49b  |
| Organism | Marinobacter shengliensis strain D49 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP028064 (4086..4190 [-], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 64..71, 74..81 (CACCATGC..GCATGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_pMAD49b
GCCCCGCAGGGCAGGATGTCCGTTGAGCACCGTCAGGTGCGAATAAGGCTCAGTGAAGAGGAACACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCCG
GCCCCGCAGGGCAGGATGTCCGTTGAGCACCGTCAGGTGCGAATAAGGCTCAGTGAAGAGGAACACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10614 | GenBank | WP_286256051 |
| Name | traI_QUE75_RS20495_pMAD49b |
UniProt ID | _ |
| Length | 590 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 590 a.a. Molecular weight: 65603.52 Da Isoelectric Point: 10.4009
>WP_286256051.1 TraI/MobA(P) family conjugative relaxase [Marinobacter shengliensis]
MIAKHVPMRSLGKSDFAGLVRYISDAQSKDHRLGHVQVTNCEAGSVRDAITEVLATQLLNTRAASDKTYH
LIVSFRAGEQPDTDTLKAIEERICNGLGYGEHQRVSAVHNDTDNLHIHIAINKIHPTRHTIHEPYYPHRT
LADLCEVLERDYNLERDNHQPRQRGTAARAADMEQHAGIESLVGWIKRECLEEIRSAASWAELHQVMRDN
GLEITPRGNGLVIRADDGTMVKPSTLARDLSKPALEKRLGAFEASPEQVERKQAKRKYQKKPVRMRVDTT
ELYARYQSEQKGLSAARAEALAEAKRRKDRAVEDAKRKAATKRAAIKLATGGVTKRLLYSQTSAGLRNDL
EAIRKQYDQERTKLYQGFQRRQWADWLKQEAMNGDSQALDALRAREAAQGLKGDTLTGQGKAKPGHAPVI
DNITKKGTIIYRAGKTAVRDDGNRLQVSCGAGGDGVQVALRMAMERYGECLTVNGSPEFKAAVIRAAVDS
KMPITFADPGLEKRRQALIEKARRPGVPPVGQEPPPHRRNGLRNLSQVEGLRIEGEAVQKPAPAPTQKPA
GVSEQEQRKAKLRAEMEAKKARRQGKGRGR
MIAKHVPMRSLGKSDFAGLVRYISDAQSKDHRLGHVQVTNCEAGSVRDAITEVLATQLLNTRAASDKTYH
LIVSFRAGEQPDTDTLKAIEERICNGLGYGEHQRVSAVHNDTDNLHIHIAINKIHPTRHTIHEPYYPHRT
LADLCEVLERDYNLERDNHQPRQRGTAARAADMEQHAGIESLVGWIKRECLEEIRSAASWAELHQVMRDN
GLEITPRGNGLVIRADDGTMVKPSTLARDLSKPALEKRLGAFEASPEQVERKQAKRKYQKKPVRMRVDTT
ELYARYQSEQKGLSAARAEALAEAKRRKDRAVEDAKRKAATKRAAIKLATGGVTKRLLYSQTSAGLRNDL
EAIRKQYDQERTKLYQGFQRRQWADWLKQEAMNGDSQALDALRAREAAQGLKGDTLTGQGKAKPGHAPVI
DNITKKGTIIYRAGKTAVRDDGNRLQVSCGAGGDGVQVALRMAMERYGECLTVNGSPEFKAAVIRAAVDS
KMPITFADPGLEKRRQALIEKARRPGVPPVGQEPPPHRRNGLRNLSQVEGLRIEGEAVQKPAPAPTQKPA
GVSEQEQRKAKLRAEMEAKKARRQGKGRGR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 6245 | GenBank | WP_036514583 |
| Name | WP_036514583_pMAD49b |
UniProt ID | W9UWQ3 |
| Length | 125 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 125 a.a. Molecular weight: 13380.32 Da Isoelectric Point: 7.9638
>WP_036514583.1 MULTISPECIES: conjugal transfer transcriptional regulator TraJ [Gammaproteobacteria]
MEANETKVTRKGSTPIKVYCLPEERAAIEANAKAAGLSTSAYLLAVGQGYRVQGVTDFENVRELVRVNGD
LGRLGGLLKLWLTDDAKTAQFGADTVLALLARIEATQDKMSGIMESVVRPRGGQA
MEANETKVTRKGSTPIKVYCLPEERAAIEANAKAAGLSTSAYLLAVGQGYRVQGVTDFENVRELVRVNGD
LGRLGGLLKLWLTDDAKTAQFGADTVLALLARIEATQDKMSGIMESVVRPRGGQA
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | W9UWQ3 |
Host bacterium
| ID | 17083 | GenBank | NZ_AP028064 |
| Plasmid name | pMAD49b | Incompatibility group | - |
| Plasmid size | 13198 bp | Coordinate of oriT [Strand] | 4086..4190 [-] |
| Host baterium | Marinobacter shengliensis strain D49 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |