Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116644
Name   oriT_AGR_28F|unnamed1 in_silico
Organism   Serratia proteamaculans strain AGR_28F
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT039179 (22703..22785 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_AGR_28F|unnamed1
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAAGGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10609 GenBank   WP_241390972
Name   traI_MOP11_RS00150_AGR_28F|unnamed1 insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73412.43 Da        Isoelectric Point: 7.3358

>WP_241390972.1 TraI/MobA(P) family conjugative relaxase [Serratia proteamaculans]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDSPLEPEHPGWRRPRSKDAIFNHLVDYISRNGDVDLQQI
MHADPNGHTQVMFDGVMCETNCFNIATASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKVADDCFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKNLTPSGAKTLEYYADVESLHTYAVSEXGFKIDEAMADPNLAWKDIHCILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLNCLEKDLGQFQLLENAGTYTKDHTTTAADAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKNAWKCPKLDAGAVKRRYQNESKRFAWQKARARVAIGDP
LLRKLTYHIIEVERMKAMAALRLTVKDERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPLSTNSIVCAVADDTPAFALEGYATRVTRDGTVQYVRDGRVQLQDKGERIDVGDHRVNEGEHIA
GGMALAENKSGEHLKFEGEAAFVQQACSMVPWFNEGGDAPLPLSDPQQRTMAGYESTYQSASTGEGQRKP
SAVEQPEVEQNKPRPGHRPQQ

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(55-320)

Large polyvalent protein-associated domain 7 (LPD7)

(518-589)


  Protein structure



No available structure.




T4CP

ID   12395 GenBank   WP_241390158
Name   trbC_MOP11_RS00185_AGR_28F|unnamed1 insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81527.35 Da        Isoelectric Point: 8.3675

>WP_241390158.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Serratia]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIAGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELISKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFELARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSALPMRINRFIDVETPTEAELFALNPSLQRKLPPTAQEVDG
ILQRLDLAVDTTASVGIMDPVLKRVAAVALDLDNRADVSYSPAQRSVLLFEAAREALHKNKRKWRQLPTP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33581..61861

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOP11_RS00175 30102..30734 + 633 WP_318035688 hypothetical protein -
MOP11_RS00180 (28Fp_00108) 30870..31355 - 486 WP_241389866 phospholipase D-like domain-containing protein -
MOP11_RS00185 (28Fp_00109) 31366..33543 - 2178 WP_241390158 F-type conjugative transfer protein TrbC -
MOP11_RS00190 (28Fp_00110) 33581..34054 - 474 WP_241390973 hypothetical protein trbB
MOP11_RS00195 (28Fp_00111) 34059..34580 - 522 WP_241390974 hypothetical protein trbB
MOP11_RS00200 (28Fp_00112) 34593..35903 - 1311 WP_241390430 conjugal transfer protein TrbA trbA
MOP11_RS00205 (28Fp_00113) 36032..36610 - 579 WP_241389923 hypothetical protein -
MOP11_RS00210 (28Fp_00114) 36607..38901 - 2295 WP_261097221 DotA/TraY family protein traY
MOP11_RS00215 (28Fp_00115) 38816..39379 - 564 WP_241390311 conjugal transfer protein TraX -
MOP11_RS00220 (28Fp_00116) 39372..40598 - 1227 WP_241390975 conjugal transfer protein TraW traW
MOP11_RS00225 (28Fp_00118) 40739..43813 - 3075 WP_241390313 type IV secretory system conjugative DNA transfer family protein traU
MOP11_RS00230 (28Fp_00119) 43816..44421 - 606 WP_241389875 hypothetical protein traT
MOP11_RS00235 (28Fp_00120) 44508..44909 - 402 WP_241389604 DUF6750 family protein traR
MOP11_RS00240 (28Fp_00121) 44925..45455 - 531 WP_241389877 conjugal transfer protein TraQ traQ
MOP11_RS00245 (28Fp_00122) 45579..46208 - 630 WP_241390976 hypothetical protein traP
MOP11_RS00250 (28Fp_00123) 46208..47440 - 1233 WP_241389929 conjugal transfer protein TraO traO
MOP11_RS00255 (28Fp_00124) 47444..48451 - 1008 WP_241390316 DotH/IcmK family type IV secretion protein traN
MOP11_RS00260 (28Fp_00125) 48454..49128 - 675 WP_241390317 DotI/IcmL/TraM family protein traM
MOP11_RS00265 (28Fp_00126) 49219..49710 - 492 WP_010895743 hypothetical protein traL
MOP11_RS00270 (28Fp_00127) 49720..50307 - 588 WP_241390977 hypothetical protein -
MOP11_RS00275 (28Fp_00128) 50241..52955 - 2715 WP_241390978 LPD7 domain-containing protein -
MOP11_RS00280 (28Fp_00130) 53098..53364 - 267 WP_241390319 IcmT/TraK family protein traK
MOP11_RS00285 (28Fp_00131) 53364..54521 - 1158 WP_241389933 plasmid transfer ATPase TraJ virB11
MOP11_RS00290 (28Fp_00133) 54711..55499 - 789 WP_010895747 type IV secretory system conjugative DNA transfer family protein traI
MOP11_RS00295 (28Fp_00134) 55496..55954 - 459 WP_010895748 DotD/TraH family lipoprotein -
MOP11_RS00300 (28Fp_00135) 55992..57425 - 1434 WP_010895749 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
MOP11_RS00305 (28Fp_00136) 57422..58090 - 669 WP_241390170 prepilin peptidase -
MOP11_RS00310 (28Fp_00137) 58090..58575 - 486 WP_010895751 lytic transglycosylase domain-containing protein virB1
MOP11_RS00315 (28Fp_00138) 58630..59223 - 594 WP_010895752 type 4 pilus major pilin -
MOP11_RS00320 (28Fp_00139) 59259..60371 - 1113 WP_241389818 type II secretion system F family protein -
MOP11_RS00325 (28Fp_00140) 60371..61861 - 1491 WP_241389888 GspE/PulE family protein virB11
MOP11_RS00330 (28Fp_00141) 61867..62451 - 585 WP_241390979 type IV pilus biogenesis protein PilP -
MOP11_RS00335 (28Fp_00142) 62438..63748 - 1311 WP_241390980 type 4b pilus protein PilO2 -
MOP11_RS00340 (28Fp_00143) 63752..65386 - 1635 WP_241389891 PilN family type IVB pilus formation outer membrane protein -
MOP11_RS00345 (28Fp_00144) 65408..65833 - 426 WP_241389892 type IV pilus biogenesis protein PilM -
MOP11_RS00350 (28Fp_00145) 65833..66846 - 1014 WP_241390981 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   17077 GenBank   NZ_MT039179
Plasmid name   AGR_28F|unnamed1 Incompatibility group   -
Plasmid size   144734 bp Coordinate of oriT [Strand]   22703..22785 [+]
Host baterium   Serratia proteamaculans strain AGR_28F

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -