Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116622
Name   oriT_pV1225-2 in_silico
Organism   Enterococcus faecium isolate V1225
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP083914 (6899..6921 [+], 23 nt)
oriT length   23 nt
IRs (inverted repeats)     _
Location of nic site      12..13
Conserved sequence flanking the
  nic site    		  GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 23 nt

>oriT_pV1225-2
AAGTCTAGTGTGTTATACTTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10593 GenBank   WP_002331393
Name   Rep_trans_LCG89_RS14515_pV1225-2 insolico UniProt ID   A0A125W9H6
Length   312 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 312 a.a.        Molecular weight: 37084.59 Da        Isoelectric Point: 9.5266

>WP_002331393.1 MULTISPECIES: replication initiation factor domain-containing protein [Bacilli]
MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFDAMTIVGNLNKTNAKKLSDFMSVEPQI
RLWDILQTKFKAKALQEKVYIEYDKVKADTWDRRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLD
LAFDFEDDLSDYYAMTDKAVKKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWR
VEVELKRDMVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIREISPI
DLTELMKSTLKANEKQLQKQIDFWQREFRFWK

  Protein domains


Predicted by InterproScan.

Replication initiation factor (Rep_trans)

(136-283)


  Protein structure


Source ID Structure
AlphaFold DB A0A125W9H6


Host bacterium

ID   17055 GenBank   NZ_CP083914
Plasmid name   pV1225-2 Incompatibility group   -
Plasmid size   48157 bp Coordinate of oriT [Strand]   6899..6921 [+]
Host baterium   Enterococcus faecium isolate V1225

Cargo genes

Drug resistance gene   erm(B), cat(pC221), VanHAX, ant(6)-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -