Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116610
Name   oriT_pKA36387-KPC2 in_silico
Organism   Klebsiella aerogenes strain NRZ-36387
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP104947 (133470..133515 [+], 46 nt)
oriT length   46 nt
IRs (inverted repeats)      6..13, 16..23  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 46 nt

>oriT_pKA36387-KPC2
AATCTGCAAAATTTTAATTTTGCGTGGTGTGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10586 GenBank   WP_001568074
Name   traI_KKS27_RS00530_pKA36387-KPC2 insolico UniProt ID   A0A9Q9P2W5
Length   1751 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1751 a.a.        Molecular weight: 191075.29 Da        Isoelectric Point: 6.1600

>WP_001568074.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIGSVKSAGKAGDYYTKEDNYYVIGSMDERWQGKGAEALGLEGKTVDKALFTELLKGKLPDGSDLSRI
QDGMNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIEAHNQAVTEAIRQVETLAATRVMVDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDTVGKTGFIENVYANQIAFGKIYREVLKPLVE
GLGYETEVVGKHGMWEMKDVPVEPFSTRTQEINAAAGPDASLKSRDVATLDTRKAKETLDPAQKVTEWLS
TLKETGFNLKEYREAADQRAKTQREVPSPEAPAGPNVDDIVTQAISGLSDRKVQFTYTELLARTVSQMEA
RPGVIETARAGIEAAIGREQLIPLDREKGIFTSGIHVLDELSVRALSQEVLRQNHVSMVPEKATARQTSF
SDAVTVLAQDRPAIGIVSGQGGAAGQRERVTEMAMMAREQGRDVHIIAADNRSLEFLLKEEKLAGEIITG
KSALQDGTAFVPGGTLIIDQAEKLSLKETINLLDGAMRNNVQILMSDSGKRSGTGSALTVMKESGVTAYK
WQGGKQTTASVISEPDRNQRFTRMASDFVASVRDGQESVVQIRGPREQGILTGLIRENLRNEGLIGSHEE
TITALTPVWLDSKNRGIRDNYREGMVMERWDPEKRIHDRFIIDRVTAVSNTLTLKDEKGESLNLKVSAVD
NTWTLFRPQQLPVAEGERLAILGKIPETRLKGGDTVQVLKAENGVLTVQHPGQKTTQKLAAGNTPFTGIK
VGHGWVENPGRSVSETATVFASLSQRELDNATLNALAQSGSQVRLYSAQNEQKTVEKLGRHTAFSVVTEQ
LKTRAGEQDISAAIAHQKSQLHTPAEQAIHLAVPSLESDKLTFTRPQLLVAAHEFSDGTVPFSDIEKTIG
EQIKSGDLLHVHVAAGHGNDLLISRQTWDAEKSLLTMVLEGKNAVTPLMAKVPGTLMEGLTSGQRNATRM
ILETRDRFTLVQGYAGVGKTTQFRAVMSAVNLLPEDSRPKVIGLGPTHRAVGEMQSAGVEAQTTASFLHD
TQLLMRSGQTPDFSNTLFLVDESSMLGLSDTAKAMSLIAAGGGRAVSSGDTDQLQSIAPGQPFRLMQKRS
AADVAIMQEIVRQVPELRPAVYSLIDRDIHSALSTIERVEPAQVPRKENAWVPESSVMEFTPEQEKAIQK
AIGEGEVLPSISPVSLYDAIVRDYTGRTESAQAQTLIITHLNDDRRVLNRMIHDEKMRNGELGEDSVTLP
VLVTSNIRDGELRKVSTWEAHKGAIALVDNQYHQIVGVSKADQLITLRSDEGTERLISPREASAEGVTLY
RQESITVSPGDRMRFSKSDTERGYVANSVWRVESISGDSVTLNDGKSTRTVTPAADKAEAHVDLAYAITT
HGAQGASEPFSIELEGVTGARERMVSFESAYVALSRMKQHTQVYTDDRKGWTDAVSRSQEKASAHDVLEP
LNDRAVKSAEQMFGRAIPLTESPAGRAALQQSGIARGQSTGKFISPGKKYPLPHVALPAFDRNGKSAGIW
LSPLTDKDGRLQAIGGEGRVMGNEEARFVALQGSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPGAITGGRVWGDIDAVVTGGSGANIPLPPDLLAQKTAEELQRKQIEKQAQETARELTGEKRKQDEVGE
QIKDIITDVVRGLERQKGQHERFQLPDEPENRLRNEAVQKVVADNQQQDRLQQIERDMVRDLTREKTLGG
D

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

TrwC relaxase (TrwC)

(10-284)

AAA domain (AAA_30)

(970-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-627)

DNA helicase TraI (TraI_C)

(1432-1554)


  Protein structure



No available structure.




Auxiliary protein

ID   6213 GenBank   WP_263433060
Name   WP_263433060_pKA36387-KPC2 insolico UniProt ID   _
Length   128 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 128 a.a.        Molecular weight: 14939.04 Da        Isoelectric Point: 5.0657

>WP_263433060.1 conjugal transfer relaxosome DNA-binding protein TraM [Klebsiella aerogenes]
MARVQAYPSDEVVDKINAIVEKRRAEVRRKKDISFSSVATMLLELGLRVYEAQMERKESGFNQKEFNKVL
LENVMKTQFTISKVLAMDSLSPHINGDDRFDFKSMVMNIRDDAREVVERFFPSQDEEE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-125)


  Protein structure



No available structure.




T4CP

ID   12369 GenBank   WP_001568075
Name   traD_KKS27_RS00535_pKA36387-KPC2 insolico UniProt ID   _
Length   735 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 735 a.a.        Molecular weight: 82704.57 Da        Isoelectric Point: 5.9222

>WP_001568075.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRFRMFGQIANIILYVLFLFFWVLCGLILMYRLSWQTFVNGAVYWWCTTLGPMR
DIIRSQSVYTINYYGQQLQYTSEQILKDKYTIWCGEQLWTGFVFAGTVSLIICIVAFFVASWVLGHQGKQ
QSEDEVTGGRQLSEKPKEVARKMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSIDKILNPLDSRCAAWDLWKECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRSVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKGIA
EFAAGEIGEKEIKKASENYSYGADPVRDGVSTGKEQKRETIVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRQLNQAIDDKLAAVLAAREAEGQSARILFMPDPVETVPVEKTDEKPASPIAAVPTP
QEDKAKVPPVTASNTFHKPASAAAAAASASVTQAGGVEQELHEKPEEQLPLPPGVNKDGEIEDMNAWDEW
QSSSDVLRDMHRREEVNINHSHHVDRDDINLGSNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 75034..85359

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKS27_RS00325 (KKS27_p00330) 70810..71790 + 981 WP_000019473 IS5-like element ISKpn26 family transposase -
KKS27_RS00330 (KKS27_p00335) 72128..73009 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
KKS27_RS00335 (KKS27_p00340) 73259..74578 - 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
KKS27_RS00340 (KKS27_p00345) 74930..75034 - 105 Protein_70 phospholipase D family protein -
KKS27_RS00345 (KKS27_p00350) 75034..76029 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
KKS27_RS00350 (KKS27_p00355) 76071..77231 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
KKS27_RS00355 (KKS27_p00360) 77231..78115 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
KKS27_RS00360 (KKS27_p00365) 78126..78824 - 699 WP_000646594 virB8 family protein virB8
KKS27_RS00365 (KKS27_p00375) 79043..79546 - 504 WP_263433057 type IV secretion system protein virB6
KKS27_RS00370 (KKS27_p00380) 79558..79752 - 195 Protein_76 hypothetical protein -
KKS27_RS00375 (KKS27_p00385) 79673..80080 - 408 Protein_77 type IV secretion system protein -
KKS27_RS00380 (KKS27_p00390) 80096..80322 - 227 Protein_78 IncN-type entry exclusion lipoprotein EexN -
KKS27_RS00385 (KKS27_p00395) 80330..81043 - 714 WP_001749960 type IV secretion system protein virB5
KKS27_RS00390 (KKS27_p00400) 81061..83661 - 2601 WP_012561149 VirB4 family type IV secretion/conjugal transfer ATPase virb4
KKS27_RS00395 (KKS27_p00405) 83661..83978 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
KKS27_RS00400 (KKS27_p00410) 84029..84322 - 294 WP_001749962 hypothetical protein virB2
KKS27_RS00405 (KKS27_p00415) 84332..84613 - 282 WP_016338364 transcriptional repressor KorA -
KKS27_RS00410 (KKS27_p00420) 84622..85359 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
KKS27_RS00415 (KKS27_p00425) 85468..85773 + 306 WP_012561154 H-NS family nucleoid-associated regulatory protein -
KKS27_RS00420 (KKS27_p00430) 85789..86133 + 345 WP_012561155 hypothetical protein -
KKS27_RS00425 (KKS27_p00435) 86130..86444 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
KKS27_RS00430 (KKS27_p00440) 86480..86791 + 312 WP_013279382 hypothetical protein -
KKS27_RS00435 (KKS27_p00445) 86847..87107 + 261 WP_016359294 hypothetical protein -
KKS27_RS00440 (KKS27_p00450) 87148..88116 - 969 WP_016151349 IS5 family transposase -
KKS27_RS00445 (KKS27_p00460) 88200..88553 + 354 WP_225622145 restriction endonuclease -
KKS27_RS00450 (KKS27_p00465) 88558..88764 + 207 WP_001749967 hypothetical protein -
KKS27_RS00455 (KKS27_p00470) 88775..89047 - 273 Protein_93 IS1 family transposase -

Region 2: 108469..134072

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKS27_RS00535 (KKS27_p00550) 108469..110676 - 2208 WP_001568075 type IV conjugative transfer system coupling protein TraD virb4
KKS27_RS00540 (KKS27_p00555) 110861..111589 - 729 Protein_110 conjugal transfer complement resistance protein TraT -
KKS27_RS28850 (KKS27_p00560) 111767..112293 - 527 Protein_111 conjugal transfer protein TraS -
KKS27_RS00545 (KKS27_p00565) 112299..115148 - 2850 WP_263433058 conjugal transfer mating-pair stabilization protein TraG traG
KKS27_RS00550 (KKS27_p00570) 115148..116518 - 1371 WP_023332869 conjugal transfer pilus assembly protein TraH traH
KKS27_RS00555 (KKS27_p00575) 116505..117041 - 537 WP_032160235 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
KKS27_RS00560 (KKS27_p00580) 117061..117294 - 234 WP_001568081 type-F conjugative transfer system pilin chaperone TraQ -
KKS27_RS00565 (KKS27_p00585) 117305..118054 - 750 WP_001568082 type-F conjugative transfer system pilin assembly protein TraF traF
KKS27_RS00570 (KKS27_p00590) 118070..118402 - 333 WP_001568083 hypothetical protein -
KKS27_RS00575 (KKS27_p00595) 118399..118659 - 261 WP_001568084 conjugal transfer protein TrbE -
KKS27_RS00580 (KKS27_p00600) 118672..120563 - 1892 Protein_119 type-F conjugative transfer system mating-pair stabilization protein TraN -
KKS27_RS00585 (KKS27_p00605) 120560..121186 - 627 WP_213492003 type-F conjugative transfer system pilin assembly protein TrbC trbC
KKS27_RS00590 (KKS27_p00610) 121199..122191 - 993 WP_023332868 conjugal transfer pilus assembly protein TraU traU
KKS27_RS00595 (KKS27_p00615) 122202..122831 - 630 WP_001568088 type-F conjugative transfer system protein TraW traW
KKS27_RS00600 (KKS27_p00620) 122828..123205 - 378 WP_001568089 type-F conjugative transfer system protein TrbI -
KKS27_RS00605 (KKS27_p00625) 123205..125844 - 2640 WP_001568090 type IV secretion system protein TraC virb4
KKS27_RS00610 (KKS27_p00635) 126305..126703 - 399 WP_001568092 hypothetical protein -
KKS27_RS00615 (KKS27_p00640) 126708..127121 - 414 WP_001568093 hypothetical protein -
KKS27_RS00620 (KKS27_p00645) 127235..127804 - 570 WP_001568094 type IV conjugative transfer system lipoprotein TraV traV
KKS27_RS00625 (KKS27_p00650) 128084..129463 - 1380 Protein_128 F-type conjugal transfer pilus assembly protein TraB -
KKS27_RS00630 (KKS27_p00655) 129463..130203 - 741 WP_001568101 type-F conjugative transfer system secretin TraK traK
KKS27_RS00635 (KKS27_p00660) 130190..130756 - 567 WP_001568102 type IV conjugative transfer system protein TraE traE
KKS27_RS00640 (KKS27_p00665) 130775..131080 - 306 WP_024191986 type IV conjugative transfer system protein TraL traL
KKS27_RS00645 (KKS27_p00670) 131094..131462 - 369 WP_001568105 type IV conjugative transfer system pilin TraA -
KKS27_RS00650 (KKS27_p00675) 131541..131705 - 165 WP_071594636 TraY domain-containing protein -
KKS27_RS00655 (KKS27_p00680) 132013..132387 - 375 WP_263433059 hypothetical protein -
KKS27_RS00660 (KKS27_p00685) 132783..133169 - 387 WP_263433060 conjugal transfer relaxosome DNA-binding protein TraM -
KKS27_RS00665 (KKS27_p00690) 133587..134072 + 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
KKS27_RS00670 (KKS27_p00695) 134105..134430 - 326 Protein_137 DUF5983 family protein -
KKS27_RS00675 (KKS27_p00700) 134463..135283 - 821 Protein_138 DUF932 domain-containing protein -
KKS27_RS00680 (KKS27_p00705) 136089..136486 - 398 Protein_139 hypothetical protein -
KKS27_RS00685 (KKS27_p00710) 136662..137472 - 811 Protein_140 type III restriction endonuclease subunit R -
KKS27_RS00690 (KKS27_p00715) 137544..138520 + 977 Protein_141 IS5 family transposase -
KKS27_RS28855 138519..138677 + 159 Protein_142 EAL domain-containing protein -

Host bacterium

ID   17043 GenBank   NZ_CP104947
Plasmid name   pKA36387-KPC2 Incompatibility group   IncFIA
Plasmid size   165682 bp Coordinate of oriT [Strand]   133470..133515 [+]
Host baterium   Klebsiella aerogenes strain NRZ-36387

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib, aac(3)-IId, blaTEM-1B, blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -