Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116603
Name   oriT_pKV33665-KPC2 in_silico
Organism   Klebsiella variicola strain NRZ-33665
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP104939 (42319..42418 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)      77..82, 90..95  (AAAAAA..TTTTTT)
 78..83, 90..95  (AAAAAA..TTTTTT)
 79..84, 90..95  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 100 nt

>oriT_pKV33665-KPC2
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGAAAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10581 GenBank   WP_012561142
Name   mobF_KKR95_RS00270_pKV33665-KPC2 insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   6205 GenBank   WP_001749975
Name   WP_001749975_pKV33665-KPC2 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   12361 GenBank   WP_000342688
Name   t4cp2_KKR95_RS00265_pKV33665-KPC2 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 6008..16337

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKR95_RS00010 (KKR95_p00010) 1983..2843 + 861 WP_000027057 broad-spectrum class A beta-lactamase TEM-1 -
KKR95_RS00015 (KKR95_p00015) 3102..3983 + 882 WP_004199234 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
KKR95_RS00020 (KKR95_p00020) 4233..5552 - 1320 WP_004152397 IS1182-like element ISKpn6 family transposase -
KKR95_RS00025 (KKR95_p00025) 5904..6008 - 105 Protein_4 phospholipase D family protein -
KKR95_RS00030 (KKR95_p00030) 6008..7003 - 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
KKR95_RS00035 (KKR95_p00035) 7045..8205 - 1161 WP_000101710 type IV secretion system protein VirB10 virB10
KKR95_RS00040 (KKR95_p00040) 8205..9089 - 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
KKR95_RS00045 (KKR95_p00045) 9100..9798 - 699 WP_000646594 virB8 family protein virB8
KKR95_RS00050 (KKR95_p00055) 10017..11057 - 1041 WP_001749958 type IV secretion system protein virB6
KKR95_RS00055 (KKR95_p00060) 11073..11300 - 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
KKR95_RS00060 (KKR95_p00065) 11308..12021 - 714 WP_001749960 type IV secretion system protein virB5
KKR95_RS00065 (KKR95_p00070) 12039..14639 - 2601 WP_012561149 VirB4 family type IV secretion/conjugal transfer ATPase virb4
KKR95_RS00070 (KKR95_p00075) 14639..14956 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
KKR95_RS00075 (KKR95_p00080) 15007..15300 - 294 WP_001749962 hypothetical protein virB2
KKR95_RS00080 (KKR95_p00085) 15310..15591 - 282 WP_016338364 transcriptional repressor KorA -
KKR95_RS00085 (KKR95_p00090) 15600..16337 - 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
KKR95_RS00090 (KKR95_p00095) 16446..16751 + 306 WP_012561154 H-NS family nucleoid-associated regulatory protein -
KKR95_RS00095 (KKR95_p00100) 16767..17111 + 345 WP_012561155 hypothetical protein -
KKR95_RS00100 (KKR95_p00105) 17108..17422 + 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
KKR95_RS00105 (KKR95_p00110) 17458..17769 + 312 WP_013279382 hypothetical protein -
KKR95_RS00110 (KKR95_p00115) 17825..18085 + 261 WP_016359294 hypothetical protein -
KKR95_RS00115 (KKR95_p00120) 18127..19095 - 969 WP_016151349 IS5 family transposase -
KKR95_RS00120 (KKR95_p00130) 19179..19532 + 354 WP_225622145 restriction endonuclease -
KKR95_RS00125 (KKR95_p00135) 19537..19743 + 207 WP_001749967 hypothetical protein -
KKR95_RS00130 (KKR95_p00140) 19754..20026 - 273 Protein_25 IS1 family transposase -

Host bacterium

ID   17036 GenBank   NZ_CP104939
Plasmid name   pKV33665-KPC2 Incompatibility group   IncN
Plasmid size   59478 bp Coordinate of oriT [Strand]   42319..42418 [-]
Host baterium   Klebsiella variicola strain NRZ-33665

Cargo genes

Drug resistance gene   blaTEM-1B, blaKPC-2, aph(6)-Id, aph(3'')-Ib, aac(3)-IId
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -