Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116547
• Name: oriT_pHMC8C-1-poxtA
• Organism: Enterococcus faecium strain HMC8C-1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP137475 (46545..46582 [+], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pHMC8C-1-poxtA
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_R5U37_RS00265_pHMC8C-1-poxtA

• ID: 10565
• GenBank: WP_000119405
• Name: mobV_R5U37_RS00265_pHMC8C-1-poxtA
• UniProt ID: F5X340
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49660.33 Da
• Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: F5X340

Host bacterium

• ID: 16980
• GenBank: NZ_CP137475
• Plasmid name: pHMC8C-1-poxtA
• Incompatibility group: -
• Plasmid size: 49249 bp
• Coordinate of oriT [Strand]: 46545..46582 [+]
• Host baterium: Enterococcus faecium strain HMC8C-1

Cargo genes

• Drug resistance gene: fexB, poxtA, erm(B), tet(M), tet(L)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -