Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116488
Name   oriT_FDAARGOS_1480|unnamed2 in_silico
Organism   Legionella anisa strain FDAARGOS_1480
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP082854 (5043..5075 [-], 33 nt)
oriT length   33 nt
IRs (inverted repeats)      3..9, 13..19  (ACGTTGC..GCAACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT_FDAARGOS_1480|unnamed2
GCACGTTGCAAAGCAACGTATAAGCGCGCACTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10537 GenBank   WP_019232995
Name   traA_K8O89_RS19120_FDAARGOS_1480|unnamed2 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101374.44 Da        Isoelectric Point: 7.0036

>WP_019232995.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Legionella]
MAIYRFSAQPISRSQGRCVVASSAYRSGEKITDERTGTIYDYTGKNDVVHKEIFLPENAPRWMSERSQLW
NAVELGEHRKDAQLAREVQLALPRELTIEQNTELVREFVQSEFVARGMVADFCFHNPKGDDGLTQPHAHI
LLTLREVNEEGFGLKERAWNDKALLEHWREEWANCQNRHLALHGIDQRVDHRSYKEQGIPLEPQYKIGAK
CAQQHMARLEDHQRIAKENGQLIFERPEIALDALTRNQSTFTHQDLARFINRHTEDAEQFKLVFERVKSC
DELVLLGVDSEGRQRFSTRDMLSIESSMMKQADKLHHQKGHEVDEHALLHAKSNRTLSQEQEVALDYVTS
SGDMKSLLGYAGTGKSYLLGAAREAWEQSGYRVQGAALSGIAALNLSDSSGIESRTIASLFYRLDKGMLT
LNSRDILVVDEAGMLGSRTMERLTREVEKASAKLVLVGDWQQLQAIEAGASFRSIAENYQYAELNQIRRQ
TTPWQVEASFNLAKGDVAHALHAYDIHDHIHRFRTTSEAKHSLIEQWNDVRIAQRCESQIILAYTRKDVK
ELNDMARLIKRRDGELGEDVVFGMERGERAFAVNDRVYFLKREDSLSVINGTLGTIHGIDAKSGVITVAL
DRDDLNKKPQLIQVNTHYYKHMEHGYAATVYKAQGVTVDRTYVLPTKHYDAHSTYVAMTRHRKSCDVFVS
REVFAHDKALVHALNRNRAKDVTLDYTQMASEFARQRGLVSENMPLRSLLKDEHSRKSEPSLSSLMNQVM
GRDVQLEQQKLDAFERQFTKENPSEALHIANSLLSTAAYQAKSLAGLFAPYSKAIARNQLTSVQNEELIR
LIGTLPLDSQVMNAFKKDYPELSTQAELFIKRNEQQLMRIKTIDKELDL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(336-522)

MobA/MobL family (MobA_MobL)

(17-213)


  Protein structure



No available structure.




T4CP

ID   12302 GenBank   WP_019232999
Name   traD_K8O89_RS19140_FDAARGOS_1480|unnamed2 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 70946.29 Da        Isoelectric Point: 7.4043

>WP_019232999.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Legionella]
MSKDPNFKHYTRGGQISFHNLRMWDQITKTLSMICLFLWVVFTVVFAWIFIPLEKITHAVAFYYAQFLSV
IGQKHHFELSFHGKTYKQSVDTILHYAYYQDNANHVINSLGKSAFWAFCLAFILGLGLAYYFIKRGKEQS
DSQYVRGSQLKNSNEVKKKILRDKAHSDIRIDQFPLIRDSEVQHLLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIIYDKGCSFIPHYYQDESDVILNPFDKRCANWDMWLEAPRDSDFENMAESSIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDRSLDKFLKLILTDEFSALEEYLQGTPAATLVSGKIEKTAISIRATLTTYLKSFSA
LAELNQEGKQPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLTPDRSRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGAKEIFDLLNTRFFFRSPSSDMARLVAA
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELKDLNCFVRLPGHYPITQLTLDLGFR
TTKTNGFIERSMPTTFNFSQLTSFDDHLESGTNGHMGSEPLTKNKINATLQCIEELNLEPL

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(35-126)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(171-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 9300..27255

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8O89_RS19110 (K8O89_19030) 4349..4606 - 258 WP_127908205 hypothetical protein -
K8O89_RS19115 (K8O89_19035) 4622..4918 - 297 WP_019232994 hypothetical protein -
K8O89_RS19120 (K8O89_19040) 5144..7813 + 2670 WP_019232995 Ti-type conjugative transfer relaxase TraA -
K8O89_RS19125 (K8O89_19045) 7824..8435 + 612 WP_019232996 hypothetical protein -
K8O89_RS19130 (K8O89_19050) 8524..8835 + 312 WP_019232997 type II toxin-antitoxin system HigB family toxin -
K8O89_RS19135 (K8O89_19055) 8832..9263 + 432 WP_019232998 type II toxin-antitoxin system HigA family antitoxin -
K8O89_RS19140 (K8O89_19060) 9300..11165 - 1866 WP_019232999 type IV conjugative transfer system coupling protein TraD virb4
K8O89_RS19145 (K8O89_19065) 11165..11920 - 756 WP_019233000 hypothetical protein -
K8O89_RS19150 (K8O89_19070) 11920..14751 - 2832 WP_019233001 conjugal transfer mating-pair stabilization protein TraG traG
K8O89_RS19155 (K8O89_19075) 14763..16127 - 1365 WP_019233002 conjugal transfer protein TraH traH
K8O89_RS19160 (K8O89_19080) 16124..16636 - 513 WP_223499702 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
K8O89_RS19165 (K8O89_19085) 16633..17376 - 744 WP_019233004 type-F conjugative transfer system pilin assembly protein TraF traF
K8O89_RS19170 (K8O89_19090) 17369..19075 - 1707 WP_019233005 type-F conjugative transfer system mating-pair stabilization protein TraN traN
K8O89_RS19175 (K8O89_19095) 19072..19488 - 417 WP_019233006 type-F conjugative transfer system pilin assembly protein TrbC trbC
K8O89_RS19180 (K8O89_19100) 19502..20485 - 984 WP_019233007 conjugal transfer pilus assembly protein TraU traU
K8O89_RS19185 (K8O89_19105) 20475..21110 - 636 WP_019233008 type-F conjugative transfer system protein TraW traW
K8O89_RS19190 (K8O89_19110) 21107..21445 - 339 WP_223499704 type-F conjugative transfer system protein TrbI -
K8O89_RS19195 (K8O89_19115) 21442..24030 - 2589 WP_019233010 type IV secretion system protein TraC virb4
K8O89_RS19200 (K8O89_19120) 24021..24311 - 291 WP_019233011 TraV family lipoprotein traV
K8O89_RS19205 (K8O89_19125) 24308..25675 - 1368 WP_019233012 TrbI/VirB10 family protein traB
K8O89_RS19210 (K8O89_19130) 25668..26387 - 720 WP_019233013 type-F conjugative transfer system secretin TraK traK
K8O89_RS19215 (K8O89_19135) 26380..26949 - 570 WP_019233014 type IV conjugative transfer system protein TraE traE
K8O89_RS19220 (K8O89_19140) 26959..27255 - 297 WP_019233015 type IV conjugative transfer system protein TraL traL
K8O89_RS19225 (K8O89_19145) 27269..27556 - 288 WP_019233016 hypothetical protein -
K8O89_RS19230 (K8O89_19150) 27679..28422 - 744 WP_019233017 complement resistance protein TraT -
K8O89_RS19235 (K8O89_19155) 28467..29558 - 1092 WP_019233018 hypothetical protein -
K8O89_RS19240 (K8O89_19160) 29536..29778 - 243 WP_019233019 TraY domain-containing protein -
K8O89_RS19245 (K8O89_19165) 29977..30636 + 660 WP_019233020 S24 family peptidase -
K8O89_RS19250 (K8O89_19170) 30620..31387 + 768 WP_019233021 VUT family protein -
K8O89_RS19255 (K8O89_19175) 31356..31889 - 534 WP_019233022 hypothetical protein -

Host bacterium

ID   16921 GenBank   NZ_CP082854
Plasmid name   FDAARGOS_1480|unnamed2 Incompatibility group   -
Plasmid size   48353 bp Coordinate of oriT [Strand]   5043..5075 [-]
Host baterium   Legionella anisa strain FDAARGOS_1480

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1