Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116482
• Name: oriT_pSh-fosB
• Organism: Staphylococcus hominis strain 706569/1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_ON953152 (3378..3396 [+], 19 nt)
• oriT length: 19 nt
• IRs (inverted repeats): _
• Location of nic site: 12..13
• Conserved sequence flanking the nic site: GTGCGCCCTT
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 19 nt

>oriT_pSh-fosB
CGCATAAGTGCGCCCTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_RUR89_RS00020_pSh-fosB

• ID: 10532
• GenBank: WP_002440612
• Name: Rep_trans_RUR89_RS00020_pSh-fosB
• UniProt ID: C5QPL5
• Length: 314 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 314 a.a.
• Molecular weight: 37662.80 Da
• Isoelectric Point: 7.5043

>WP_002440612.1 MULTISPECIES: replication initiation factor domain-containing protein [Bacteria]
MSRKEQEIHSKQSTLQEVTNYTSTGYSNSRLGVHSVHPQNPKLSFDAMTIVGNLSRDNAQKLSSFMSIEP
QIRLWDILQTKFKAKALQEKVYIEYDKVKADTWDRRNMRVEFNPNKLTHEEMLWLKQNIINYMEDDGFTR
IDLAFDFEDDLSNYYAMTDKAVKKTVFYGRDGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVMSDHL
WRVEIELKRDMVDYWNDCFNDLHILKPDWTSPEKLNEQAMVYMLIHEEDKWGELNKRTKYKYKNLIKEIS
PVDLTELMKSTLRENEKQLQKQIEFWQTKGKKLF

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (138-227)

Protein structure

• AlphaFold DB: C5QPL5

Host bacterium

• ID: 16915
• GenBank: NZ_ON953152
• Plasmid name: pSh-fosB
• Incompatibility group: -
• Plasmid size: 5079 bp
• Coordinate of oriT [Strand]: 3378..3396 [+]
• Host baterium: Staphylococcus hominis strain 706569/1

Cargo genes

• Drug resistance gene: fosB
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -