Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116481
Name   oriT_pF27D in_silico
Organism   Lactococcus cremoris strain F.2.7
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP068515 (12059..12195 [+], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      21..28, 42..49  (AAAAGGGG..CCCCTTTT)
 25..30, 39..44  (GGGGAA..TTCCCC)
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  GCTTGCAGTA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pF27D
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATTTCCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10531 GenBank   WP_015081763
Name   Relaxase_LLF72_RS12550_pF27D insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47655.35 Da        Isoelectric Point: 8.1230

>WP_015081763.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELEKR
TKQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   16914 GenBank   NZ_CP068515
Plasmid name   pF27D Incompatibility group   -
Plasmid size   47391 bp Coordinate of oriT [Strand]   12059..12195 [+]
Host baterium   Lactococcus cremoris strain F.2.7

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1