Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116466 |
| Name | oriT_2022JQ-00300|unnamed2  |
| Organism | Klebsiella quasipneumoniae strain 2022JQ-00300 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP136942 (137083..137132 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_2022JQ-00300|unnamed2
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10522 | GenBank | WP_023307532 |
| Name | traI_NRI38_RS27005_2022JQ-00300|unnamed2 |
UniProt ID | A0A2R4ND60 |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190476.66 Da Isoelectric Point: 6.4600
>WP_023307532.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRAMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELACAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALITIEQVTPEQVPRKEGVWAPGSSVVEFTPKQEKAIE
KALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRTLNGMIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSDGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASERYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNDAARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRIWADPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRAMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELACAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRHNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QTQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKGAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALITIEQVTPEQVPRKEGVWAPGSSVVEFTPKQEKAIE
KALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRTLNGMIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSDGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASERYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNDAARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRIWADPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 131700..137690
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NRI38_RS27015 (NRI38_27015) | 127841..128809 | - | 969 | WP_077258488 | IS5-like element IS903B family transposase | - |
| NRI38_RS27020 (NRI38_27020) | 128843..129067 | - | 225 | Protein_126 | TraC family protein | - |
| NRI38_RS27025 (NRI38_27025) | 129139..129537 | - | 399 | WP_072158599 | hypothetical protein | - |
| NRI38_RS27030 (NRI38_27030) | 129545..129835 | - | 291 | WP_023307542 | hypothetical protein | - |
| NRI38_RS27035 (NRI38_27035) | 129832..130236 | - | 405 | WP_023307543 | hypothetical protein | - |
| NRI38_RS27040 (NRI38_27040) | 130303..130620 | - | 318 | WP_023307544 | hypothetical protein | - |
| NRI38_RS27045 (NRI38_27045) | 130621..130839 | - | 219 | WP_004171484 | hypothetical protein | - |
| NRI38_RS27050 (NRI38_27050) | 130863..131153 | - | 291 | WP_032423381 | hypothetical protein | - |
| NRI38_RS27055 (NRI38_27055) | 131158..131568 | - | 411 | WP_269136663 | hypothetical protein | - |
| NRI38_RS27060 (NRI38_27060) | 131700..132269 | - | 570 | WP_023307547 | type IV conjugative transfer system lipoprotein TraV | traV |
| NRI38_RS27065 (NRI38_27065) | 132481..132894 | - | 414 | Protein_135 | conjugal transfer pilus-stabilizing protein TraP | - |
| NRI38_RS27070 (NRI38_27070) | 132887..134311 | - | 1425 | WP_023307549 | F-type conjugal transfer pilus assembly protein TraB | traB |
| NRI38_RS27075 (NRI38_27075) | 134311..135051 | - | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| NRI38_RS27080 (NRI38_27080) | 135038..135604 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| NRI38_RS27085 (NRI38_27085) | 135624..135935 | - | 312 | WP_317751745 | type IV conjugative transfer system protein TraL | traL |
| NRI38_RS27090 (NRI38_27090) | 135955..136923 | + | 969 | WP_077258488 | IS5-like element IS903B family transposase | - |
| NRI38_RS27095 (NRI38_27095) | 137205..137690 | + | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| NRI38_RS27100 (NRI38_27100) | 137723..138052 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| NRI38_RS27105 (NRI38_27105) | 138079..138906 | - | 828 | WP_023307552 | DUF932 domain-containing protein | - |
| NRI38_RS27110 (NRI38_27110) | 139736..140149 | - | 414 | WP_023280875 | type II toxin-antitoxin system HigA family antitoxin | - |
| NRI38_RS27115 (NRI38_27115) | 140150..140428 | - | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| NRI38_RS27120 (NRI38_27120) | 140418..140738 | - | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| NRI38_RS27125 (NRI38_27125) | 140819..141043 | - | 225 | WP_004152719 | hypothetical protein | - |
| NRI38_RS27130 (NRI38_27130) | 141054..141266 | - | 213 | WP_004152718 | hypothetical protein | - |
| NRI38_RS27135 (NRI38_27135) | 141327..141683 | - | 357 | WP_004152717 | hypothetical protein | - |
| NRI38_RS27140 (NRI38_27140) | 142320..142670 | - | 351 | WP_023280874 | hypothetical protein | - |
Host bacterium
| ID | 16899 | GenBank | NZ_CP136942 |
| Plasmid name | 2022JQ-00300|unnamed2 | Incompatibility group | IncFIB |
| Plasmid size | 163799 bp | Coordinate of oriT [Strand] | 137083..137132 [+] |
| Host baterium | Klebsiella quasipneumoniae strain 2022JQ-00300 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsH, arsR, arsB, arsC, fecE, fecD, silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsA, arsD |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |