Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116465 |
| Name | oriT_WUSP082|unnamed  |
| Organism | Streptococcus pasteurianus strain WUSP082 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP136944 (17045..17074 [-], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT_WUSP082|unnamed
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10521 | GenBank | WP_233701194 |
| Name | mobQ_RJD36_RS12425_WUSP082|unnamed |
UniProt ID | _ |
| Length | 661 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 661 a.a. Molecular weight: 77313.36 Da Isoelectric Point: 9.2532
>WP_233701194.1 MULTISPECIES: MobQ family relaxase [Lactobacillales]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKYID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKYID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12290 | GenBank | WP_002325630 |
| Name | t4cp2_RJD36_RS12470_WUSP082|unnamed |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63114.14 Da Isoelectric Point: 4.7040
>WP_002325630.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 19540..31728
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RJD36_RS12405 (RJD36_12405) | 15371..16051 | + | 681 | WP_014293938 | IS6-like element IS1216 family transposase | - |
| RJD36_RS12410 (RJD36_12410) | 16123..16299 | + | 177 | WP_181728217 | hypothetical protein | - |
| RJD36_RS12415 (RJD36_12415) | 16329..16586 | - | 258 | WP_010717195 | hypothetical protein | - |
| RJD36_RS12420 (RJD36_12420) | 16589..16888 | - | 300 | WP_002325624 | hypothetical protein | - |
| RJD36_RS12425 (RJD36_12425) | 17180..19165 | + | 1986 | WP_233701194 | MobQ family relaxase | - |
| RJD36_RS12430 (RJD36_12430) | 19189..19521 | + | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| RJD36_RS12435 (RJD36_12435) | 19540..19923 | + | 384 | WP_001083514 | glycosyl transferase | trsC |
| RJD36_RS12440 (RJD36_12440) | 19940..20569 | + | 630 | WP_104888277 | hypothetical protein | trsD |
| RJD36_RS12445 (RJD36_12445) | 20580..22541 | + | 1962 | WP_231428364 | VirB4 family type IV secretion system protein | virb4 |
| RJD36_RS12450 (RJD36_12450) | 22555..23907 | + | 1353 | WP_104659242 | conjugal transfer protein TraF | trsF |
| RJD36_RS12455 (RJD36_12455) | 23929..25038 | + | 1110 | WP_021109240 | lysozyme family protein | orf14 |
| RJD36_RS12460 (RJD36_12460) | 25051..25602 | + | 552 | WP_021109241 | hypothetical protein | - |
| RJD36_RS12465 (RJD36_12465) | 25607..26038 | + | 432 | WP_002301637 | hypothetical protein | - |
| RJD36_RS12470 (RJD36_12470) | 26031..27686 | + | 1656 | WP_002325630 | VirD4-like conjugal transfer protein, CD1115 family | - |
| RJD36_RS12475 (RJD36_12475) | 27704..28627 | + | 924 | WP_212481474 | hypothetical protein | - |
| RJD36_RS12480 (RJD36_12480) | 28629..29561 | + | 933 | WP_002325632 | conjugal transfer protein TrbL family protein | trsL |
| RJD36_RS12485 (RJD36_12485) | 29578..30549 | + | 972 | WP_002425009 | hypothetical protein | - |
| RJD36_RS12490 (RJD36_12490) | 30578..30946 | + | 369 | WP_000516429 | hypothetical protein | - |
| RJD36_RS12495 (RJD36_12495) | 31006..31728 | + | 723 | WP_317751537 | LPXTG cell wall anchor domain-containing protein | prgC |
| RJD36_RS12500 (RJD36_12500) | 32050..32322 | + | 273 | WP_011266100 | helix-turn-helix transcriptional regulator | - |
| RJD36_RS12505 (RJD36_12505) | 32706..34196 | + | 1491 | WP_311933771 | primase C-terminal domain-containing protein | - |
Host bacterium
| ID | 16898 | GenBank | NZ_CP136944 |
| Plasmid name | WUSP082|unnamed | Incompatibility group | - |
| Plasmid size | 34665 bp | Coordinate of oriT [Strand] | 17045..17074 [-] |
| Host baterium | Streptococcus pasteurianus strain WUSP082 |
Cargo genes
| Drug resistance gene | fexA, optrA, erm(A) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |