Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116423 |
| Name | oriT_w6|unnamed  |
| Organism | Klebsiella sp. w6 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP136908 (88293..88342 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_w6|unnamed
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10495 | GenBank | WP_317704077 |
| Name | traI_R0140_RS27755_w6|unnamed |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190693.65 Da Isoelectric Point: 6.0761
>WP_317704077.1 conjugative transfer relaxase/helicase TraI [Klebsiella sp. w6]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDHPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALGLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VDRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDHPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALGLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VDRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12251 | GenBank | WP_317704076 |
| Name | traD_R0140_RS27750_w6|unnamed |
UniProt ID | _ |
| Length | 768 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 768 a.a. Molecular weight: 85763.72 Da Isoelectric Point: 5.1854
>WP_317704076.1 type IV conjugative transfer system coupling protein TraD [Klebsiella sp. w6]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLISMGTKEDPFWQG
SGRTIFAEGAFLMRKDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKSPAAEPSARAAEPPVLRVTTVPLTKRKSAASAAAASTASSVGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRRDEQDEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLISMGTKEDPFWQG
SGRTIFAEGAFLMRKDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKSPAAEPSARAAEPPVLRVTTVPLTKRKSAASAAAASTASSVGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRRDEQDEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 87735..115977
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| R0140_RS27550 | 83779..84159 | + | 381 | WP_032437935 | hypothetical protein | - |
| R0140_RS27555 | 84226..84573 | + | 348 | WP_032437933 | hypothetical protein | - |
| R0140_RS27560 | 84668..84814 | + | 147 | WP_032437931 | hypothetical protein | - |
| R0140_RS27565 | 84865..85698 | + | 834 | WP_317704069 | N-6 DNA methylase | - |
| R0140_RS27570 | 86519..87340 | + | 822 | WP_015065622 | DUF932 domain-containing protein | - |
| R0140_RS27575 | 87388..87702 | + | 315 | WP_001568110 | DUF5983 family protein | - |
| R0140_RS27580 | 87735..88220 | - | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| R0140_RS27585 | 88655..89047 | + | 393 | WP_317704070 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| R0140_RS27590 | 89254..89949 | + | 696 | WP_317704071 | transcriptional regulator TraJ family protein | - |
| R0140_RS27595 | 90033..90404 | + | 372 | WP_317704072 | TraY domain-containing protein | - |
| R0140_RS27600 | 90458..90826 | + | 369 | WP_004178060 | type IV conjugative transfer system pilin TraA | - |
| R0140_RS27605 | 90840..91145 | + | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| R0140_RS27610 | 91165..91731 | + | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| R0140_RS27615 | 91718..92458 | + | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| R0140_RS27620 | 92458..93882 | + | 1425 | WP_317704073 | F-type conjugal transfer pilus assembly protein TraB | traB |
| R0140_RS27625 | 93875..94288 | + | 414 | Protein_116 | conjugal transfer pilus-stabilizing protein TraP | - |
| R0140_RS27630 | 94500..95069 | + | 570 | WP_015065628 | type IV conjugative transfer system lipoprotein TraV | traV |
| R0140_RS27635 | 95225..95623 | + | 399 | WP_223177007 | hypothetical protein | - |
| R0140_RS27640 | 95652..95942 | + | 291 | WP_032426790 | hypothetical protein | - |
| R0140_RS27645 | 95966..96184 | + | 219 | WP_004171484 | hypothetical protein | - |
| R0140_RS27650 | 96185..96523 | + | 339 | WP_099743479 | hypothetical protein | - |
| R0140_RS27655 | 96568..96972 | + | 405 | WP_023320104 | hypothetical protein | - |
| R0140_RS27660 | 96997..97395 | + | 399 | WP_015065631 | hypothetical protein | - |
| R0140_RS27665 | 97467..100106 | + | 2640 | Protein_124 | type IV secretion system protein TraC | - |
| R0140_RS27670 | 100106..100495 | + | 390 | WP_004152591 | type-F conjugative transfer system protein TrbI | - |
| R0140_RS27675 | 100495..101121 | + | 627 | WP_004152590 | type-F conjugative transfer system protein TraW | traW |
| R0140_RS27680 | 101165..102124 | + | 960 | WP_317704091 | conjugal transfer pilus assembly protein TraU | traU |
| R0140_RS27685 | 102137..102775 | + | 639 | WP_015065635 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| R0140_RS27690 | 102834..104789 | + | 1956 | WP_015065636 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| R0140_RS27695 | 104821..105057 | + | 237 | WP_317704074 | conjugal transfer protein TrbE | - |
| R0140_RS27700 | 105054..105239 | + | 186 | WP_032446653 | hypothetical protein | - |
| R0140_RS27705 | 105285..105611 | + | 327 | WP_016529362 | hypothetical protein | - |
| R0140_RS27710 | 105632..106384 | + | 753 | WP_015065637 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| R0140_RS27715 | 106395..106634 | + | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| R0140_RS27720 | 106606..107163 | + | 558 | WP_022644930 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| R0140_RS27725 | 107208..107651 | + | 444 | WP_015065638 | F-type conjugal transfer protein TrbF | - |
| R0140_RS27730 | 107629..109008 | + | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
| R0140_RS27735 | 109008..111857 | + | 2850 | WP_317704075 | conjugal transfer mating-pair stabilization protein TraG | traG |
| R0140_RS27740 | 111863..112390 | + | 528 | WP_317704092 | conjugal transfer protein TraS | - |
| R0140_RS27745 | 112580..113311 | + | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| R0140_RS27750 | 113671..115977 | + | 2307 | WP_317704076 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 16856 | GenBank | NZ_CP136908 |
| Plasmid name | w6|unnamed | Incompatibility group | IncFIA |
| Plasmid size | 124877 bp | Coordinate of oriT [Strand] | 88293..88342 [-] |
| Host baterium | Klebsiella sp. w6 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsH, arsA, arsC, arsB, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |