Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116405
Name   oriT_FDAARGOS_80|unnamed1 in_silico
Organism   Proteus mirabilis strain FDAARGOS_80
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP026060 (1707..1807 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      32..38, 43..49  (TTACGAT..ATCGTAA)
 21..26, 38..43  (TTTTCA..TGAAAA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_FDAARGOS_80|unnamed1
AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGTGTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10484 GenBank   WP_000932317
Name   mobF_MC72_RS18410_FDAARGOS_80|unnamed1 insolico UniProt ID   A0A626R2M1
Length   969 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 969 a.a.        Molecular weight: 108425.51 Da        Isoelectric Point: 9.7925

>WP_000932317.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLDGKVTDDISVM
RKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENT
NKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYN
LRYDRNGTFDLAHFSQEQIAQFSQRSQQIEAELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEG
WKNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAM
KHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQLKAVMGIIHT
PNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPG
SVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDP
ELLKAALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINAYIQ
AELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGS
GDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASRE
GFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGKGHDKNNDERAFTSL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(475-649)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(850-896)

TrwC relaxase (TrwC)

(14-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A626R2M1


T4CP

ID   12236 GenBank   WP_036896339
Name   t4cp2_MC72_RS18405_FDAARGOS_80|unnamed1 insolico UniProt ID   _
Length   525 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 525 a.a.        Molecular weight: 59001.90 Da        Isoelectric Point: 9.5507

>WP_036896339.1 type IV secretion system DNA-binding domain-containing protein [Proteus mirabilis]
MNEADKGKICLGALLFTPLVTWFIAAKTLYGFTPKDARERLVLLIQQTFDLWPLWGALLVGEIIAIIGLI
IFFKFNHQIFKGAPFKKIYRGTRLVSQRALASLTKEREPQITIADIPIPTKAEGTHISIAGATGVGKSTI
FAEMMKGCLIRGKMQRVASKKDRMIILDPDGDFLSRFYKKGDKILNPYDARTEGWVFFNEIKSDFDFERF
GVSIVPTAKDSQTEEWNGYGRLLFTEVSRKVFNTSRNPTMEEVFYWTNEVPLEELEEYVRGTKAQALFAG
SGRAVGSARFVLSNKLAAHLKMPAGNFSIREWLEDPKGGNLYITWTDEMREALKSLISCWTDSIFSIVLG
MSKSNTRKIWTYIDELESLDYLPSLRDALTKGRKKGLRVVTGYQSYSQVISIYGSDVAETLLSNHRTSVV
MAAGRLGERTLEFVSKSLGEIEGEREKTGISRRFGQMGTKNINDDHKRERAVTPTEIATLDDLTGYIAFP
GNLPVAKFETHHVNYTRSNPVPGVVLRESTAFAGM

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(111-499)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2249..20038

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC72_RS18390 (MC72_018390) 311..1021 - 711 WP_000686338 StbB family protein -
MC72_RS18395 (MC72_018395) 1034..1477 - 444 WP_024249956 hypothetical protein -
MC72_RS18400 (MC72_018400) 1909..2256 + 348 WP_001139329 hypothetical protein -
MC72_RS18405 (MC72_018405) 2249..3826 + 1578 WP_036896339 type IV secretion system DNA-binding domain-containing protein virb4
MC72_RS18410 (MC72_018410) 3829..6738 + 2910 WP_000932317 MobF family relaxase -
MC72_RS18415 (MC72_018415) 7016..7210 - 195 WP_000529710 hypothetical protein -
MC72_RS18420 (MC72_018420) 7222..7704 - 483 WP_036896345 GNAT family N-acetyltransferase -
MC72_RS18425 (MC72_018425) 7717..8271 - 555 WP_000616063 phospholipase D family protein -
MC72_RS18430 (MC72_018430) 8234..9265 - 1032 WP_000138004 P-type DNA transfer ATPase VirB11 virB11
MC72_RS18435 (MC72_018435) 9258..10412 - 1155 WP_001224296 type IV secretion system protein VirB10 virB10
MC72_RS18440 (MC72_018440) 10409..11296 - 888 WP_000744355 TrbG/VirB9 family P-type conjugative transfer protein virB9
MC72_RS18445 (MC72_018445) 11299..12015 - 717 WP_000914645 virB8 family protein virB8
MC72_RS19120 12018..12188 - 171 WP_021536809 hypothetical protein -
MC72_RS18450 (MC72_018450) 12283..13326 - 1044 WP_000885742 type IV secretion system protein virB6
MC72_RS18455 (MC72_018455) 13345..13581 - 237 WP_000733633 EexN family lipoprotein -
MC72_RS18460 (MC72_018460) 13585..14292 - 708 WP_036896352 type IV secretion system protein virB5
MC72_RS18465 (MC72_018465) 14302..16932 - 2631 WP_000257330 VirB4 family type IV secretion/conjugal transfer ATPase virb4
MC72_RS18470 (MC72_018470) 16932..17246 - 315 WP_001053888 type IV secretion system protein VirB3 virB3
MC72_RS18475 (MC72_018475) 17296..17586 - 291 WP_001090269 TrbC/VirB2 family protein virB2
MC72_RS18480 (MC72_018480) 17570..17887 - 318 WP_001267114 hypothetical protein -
MC72_RS18485 (MC72_018485) 17910..18650 - 741 WP_000815895 lytic transglycosylase domain-containing protein virB1
MC72_RS18490 (MC72_018490) 18713..18949 + 237 WP_000089855 H-NS family nucleoid-associated regulatory protein -
MC72_RS18495 (MC72_018495) 18961..19398 + 438 WP_000034608 hypothetical protein -
MC72_RS18500 (MC72_018500) 19404..19721 + 318 WP_001226231 TrbM/KikA/MpfK family conjugal transfer protein -
MC72_RS18505 (MC72_018505) 19724..20038 + 315 WP_000851068 hypothetical protein tfc15
MC72_RS18510 (MC72_018510) 20014..20739 + 726 WP_223307254 restriction endonuclease -
MC72_RS19460 20740..21141 + 402 WP_023187076 Hha/YmoA family nucleoid-associated regulatory protein -
MC72_RS18520 (MC72_018520) 21201..21515 - 315 WP_001280992 hypothetical protein -
MC72_RS18525 (MC72_018525) 21541..21867 - 327 WP_000817647 endoribonuclease MazF -
MC72_RS18530 (MC72_018530) 21857..22153 - 297 WP_223307255 hypothetical protein -
MC72_RS18535 (MC72_018535) 22123..22665 - 543 WP_000711249 hypothetical protein -
MC72_RS18540 (MC72_018540) 22665..22910 - 246 WP_000195442 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
MC72_RS18550 (MC72_018550) 23372..23794 + 423 WP_000838908 hypothetical protein -
MC72_RS18555 (MC72_018555) 23794..24378 + 585 WP_000690619 recombinase family protein -

Host bacterium

ID   16838 GenBank   NZ_CP026060
Plasmid name   FDAARGOS_80|unnamed1 Incompatibility group   -
Plasmid size   34615 bp Coordinate of oriT [Strand]   1707..1807 [-]
Host baterium   Proteus mirabilis strain FDAARGOS_80

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -