Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116376
Name   oriT_pK1721-KPC_79k in_silico
Organism   Citrobacter koseri strain K1721
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP136812 (51398..51479 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_pK1721-KPC_79k
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10465 GenBank   WP_032440569
Name   traI_R1158_RS22830_pK1721-KPC_79k insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


Auxiliary protein

ID   6139 GenBank   WP_062955149
Name   WP_062955149_pK1721-KPC_79k insolico UniProt ID   A0A229UZL3
Length   114 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 114 a.a.        Molecular weight: 12838.82 Da        Isoelectric Point: 10.0962

>WP_062955149.1 MULTISPECIES: MobC family plasmid mobilization relaxosome protein [Bacteria]
MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPARSLRLPSIDPVLLRQLAGMGNNLNQI
ARKINGGQWSGADRVQVVAALMAIDAELERLRHAVLEKGADDDR

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(57-101)


  Protein structure


Source ID Structure
AlphaFold DB A0A229UZL3


T4CP

ID   12215 GenBank   WP_032440593
Name   trbC_R1158_RS22690_pK1721-KPC_79k insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 20356..42289

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R1158_RS22675 (R1158_22675) 16119..16814 - 696 WP_254910806 RepA family replication protein -
R1158_RS22680 (R1158_22680) 17320..17571 - 252 WP_113394424 hypothetical protein -
R1158_RS22685 (R1158_22685) 17583..18116 - 534 WP_032440594 phospholipase D family protein -
R1158_RS22690 (R1158_22690) 18162..20354 - 2193 WP_032440593 F-type conjugative transfer protein TrbC -
R1158_RS22695 (R1158_22695) 20356..21477 - 1122 WP_052455213 thioredoxin fold domain-containing protein trbB
R1158_RS22700 (R1158_22700) 21458..22876 - 1419 WP_063666705 hypothetical protein trbA
R1158_RS22705 (R1158_22705) 22978..23601 - 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
R1158_RS22710 (R1158_22710) 23667..25811 - 2145 WP_032440591 DotA/TraY family protein traY
R1158_RS22715 (R1158_22715) 25853..26434 - 582 WP_050597335 conjugal transfer protein TraX -
R1158_RS22720 (R1158_22720) 26431..27627 - 1197 WP_032440590 conjugal transfer protein TraW traW
R1158_RS22725 (R1158_22725) 27637..30684 - 3048 WP_032440589 conjugal transfer protein traU
R1158_RS22730 (R1158_22730) 30694..31257 - 564 WP_045325070 hypothetical protein traT
R1158_RS22735 (R1158_22735) 31301..31702 - 402 WP_032440588 DUF6750 family protein traR
R1158_RS22740 (R1158_22740) 31758..32291 - 534 WP_032440587 conjugal transfer protein TraQ traQ
R1158_RS22745 (R1158_22745) 32301..33020 - 720 WP_050597333 conjugal transfer protein TraP traP
R1158_RS22750 (R1158_22750) 33027..34367 - 1341 WP_032440586 conjugal transfer protein TraO traO
R1158_RS22755 (R1158_22755) 34371..35474 - 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
R1158_RS22760 (R1158_22760) 35485..36153 - 669 WP_032440585 DotI/IcmL/TraM family protein traM
R1158_RS22765 (R1158_22765) 36225..36578 - 354 WP_032440584 hypothetical protein traL
R1158_RS22770 (R1158_22770) 36612..40025 - 3414 WP_052145516 LPD7 domain-containing protein -
R1158_RS22775 (R1158_22775) 40043..40309 - 267 WP_032440577 IcmT/TraK family protein traK
R1158_RS22780 (R1158_22780) 40306..41493 - 1188 WP_077255878 plasmid transfer ATPase TraJ virB11
R1158_RS22785 (R1158_22785) 41471..42289 - 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
R1158_RS22790 (R1158_22790) 42286..42750 - 465 WP_225546581 DotD/TraH family lipoprotein -
R1158_RS22795 (R1158_22795) 42776..43255 - 480 WP_032440574 lytic transglycosylase domain-containing protein -
R1158_RS22800 (R1158_22800) 43268..43636 - 369 WP_126523558 hypothetical protein -
R1158_RS22805 (R1158_22805) 43987..44214 - 228 WP_032440573 hypothetical protein -
R1158_RS22810 (R1158_22810) 44824..45666 + 843 WP_032440572 helix-turn-helix domain-containing protein -
R1158_RS22815 (R1158_22815) 45726..46655 + 930 WP_032440581 glycosyltransferase family 9 protein -
R1158_RS22820 (R1158_22820) 46648..47142 + 495 WP_032440571 molybdopterin-dependent oxidoreductase -

Host bacterium

ID   16809 GenBank   NZ_CP136812
Plasmid name   pK1721-KPC_79k Incompatibility group   ColRNAI
Plasmid size   79918 bp Coordinate of oriT [Strand]   51398..51479 [-]
Host baterium   Citrobacter koseri strain K1721

Cargo genes

Drug resistance gene   blaKPC-2, qnrS1, blaLAP-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -