Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116370
• Name: oriT_pK2721-4
• Organism: Citrobacter koseri strain K2721
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP136833 (2400..2437 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 7..14, 18..25 (GACTTTGC..GCAAAGTC)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pK2721-4
GGGCACGACTTTGCTTCGCAAAGTCTAGTGAGTACACT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_R1243_RS23530_pK2721-4

• ID: 10460
• GenBank: WP_033995413
• Name: mobV_R1243_RS23530_pK2721-4
• UniProt ID: A0AA97GNU2
• Length: 324 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 324 a.a.
• Molecular weight: 35777.43 Da
• Isoelectric Point: 10.3810

>WP_033995413.1 MULTISPECIES: MobV family relaxase [Bacteria]
MAAYAIMRCKKLATMGSVAAALQHCYRERETPNADAERTPDNEHRAATSTDAAMGRLRELLPEKRRKDAV
LAVEYVMTASPEWWAKATPQQQAAFFDQAQGWLAAKYGADRIVTASIHRDEATPHLSAFVVPLTQDGRLS
AKEFIGGRDKMRADQTSFAEAVRDLGLERGIEGSRATHQRVQSYYGAIERQPGHATITPQAIEPRVLRKG
IFSKDVETPEAVAARVTAAVREGYGPTVAAAAGARQEREKARQAQETARSLRDRLKPVLDALGPLNRDMQ
AKAAQIIKAVGEKLLAEQREATRQRQAQRLRSRGRDRDDGGLSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-185)

Protein structure

No available structure.

Host bacterium

• ID: 16803
• GenBank: NZ_CP136833
• Plasmid name: pK2721-4
• Incompatibility group: -
• Plasmid size: 2953 bp
• Coordinate of oriT [Strand]: 2400..2437 [-]
• Host baterium: Citrobacter koseri strain K2721

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -